Tag: rapid cycling

Usman Kadir and his family de-husk maize on their farm in Ethiopia. (Photo: Apollo Habtamu/ILRI)

Faster results at a lower cost

Written by Emma Orchardson on November 19, 2020. Posted in Blogs.

The current COVID-19 pandemic — and associated measures to reduce its spread — is projected to increase extreme poverty by 20%, with the largest increase in sub-Saharan Africa, where 80 million more people would join the ranks of the extreme poor. Accelerating the process of delivering high-quality, climate resilient and nutritionally enriched maize seed is now more critical than ever.However, developing these varieties is not a rapid or cheap process. Over the course of five years, researchers on the Stress Tolerant Maize for Africa (STMA) project developed a range of tools and technologies to reduce the overall cost of producing a new high yielding, stress tolerant hybrids for smallholder farmers in the region.

Maize breeding starts with crossing two parents and essentially ends after testing their great-great-great-great grandchildren in as many locations as possible. This allows plant breeders to identify the new varieties which will perform well in the conditions faced by their target beneficiaries — in the case of STMA, smallholder farmers in Africa. In other parts of the world, new tools and technologies are routinely added to breeding programs to help reduce the cost and time it takes to produce new varieties.

Scientists on the STMA project focused on testing and scaling new tools specifically for maize breeding programs in sub-Saharan Africa and began by taking a closer look at the most expensive part of the breeding process: phenotyping or collecting precise information on plant traits.

“Within a breeding program, phenotyping is the single most costly step,” explains CIMMYT molecular breeder Manje Gowda. “Molecular technologies provide opportunities to reduce this cost.” The research team tested two methods to speed up this step and make it more cost efficient: forward breeding and genomic selection.

Speeding up a long and costly process

Two important traits maize breeders look for in their plant progeny are susceptibility for two key maize diseases: maize streak virus (MSV) and maize lethal necrosis (MLN). In traditional breeding, breeders must extensively test lines in the field for their susceptibility to these diseases, and then remove them before the next round of crossing. This carries a significant cost.

Using a process called forward breeding, scientists can screen for DNA markers known to be associated with susceptibility to these diseases. This allows breeders to identify lines vulnerable to these diseases and remove them before field testing.

Scientists on the STMA project applied this approach in CIMMYT breeding programs in eastern and southern Africa over the past four years, saving an estimated $300,000 in field costs. Under the AGG project, research will now focus on applying forward breeding to identify susceptibility for another fast-spreading maize pest, fall armyworm, as well as extending use of this method in partners’ breeding programs.

A CIMMYT research associate inspects maize damaged by fall army worm at KALRO Kiboko Research Station in Kenya. (Photo: Peter Lowe/CIMMYT)

Forward breeding is ideal for “simple” traits which are controlled by a few genes. However, other desired traits, such as tolerance to drought and low nitrogen stress, are genetically complex. Many genes control these traits, with each gene only contributing a little towards overall stress tolerance.

In this case, a technology called genomic selection can be of service. Genomic selection estimates the performance, or breeding value, of a line based largely on genetic information. Genomic selection uses more than 5,000 DNA markers, without the need for precise information about what traits these markers control. The method is ideal for complicated traits such as drought and low nitrogen stress tolerance, where hundreds of small effect genes together largely control how a plant grows under these stresses.

CIMMYT scientists used this technology to select and advance lines for drought tolerance. They then tested these lines and compared their performance in the field to lines selected conventionally. They found that the two sets of resulting hybrid varieties — those advanced using genomic selection and those advanced in the field — showed the same grain yield under drought stress. However, genomic selection only required phenotyping half the lines, achieving the same outcome with half the budget.

Innovations in the field

While DNA technology is reducing the need for extensive field phenotyping, research is also underway to reduce the cost of the remaining necessary phenotyping in the field.

Typically, many traits — such as plant height or leaf drying under drought stress — are measured by hand, using the labor of large teams of people. For example, plant and ear height is traditionally measured by a team of two using a meter stick.

Mainasarra Zaman-Allah, a CIMMYT abiotic stress phenotyping specialist based in Zimbabwe, has been developing faster, more accurate ways to measure these traits. He implemented the use of a small laser sensor to measure plant and ear height which only requires one person. This simple yet cost effective tool has reduced the cost of measuring these traits by almost 60%. Similarly, using a UAV-based platform has reduced the cost of measuring a trait known as canopy senescence — leaf drying associated with drought susceptibility —by over 65%.

The identification of plants which are tolerant to key diseases has traditionally involved scoring the severity of disease in each plot visually, but walking through hundreds of plots daily can lead to errors in human judgement. To combat this, CIMMYT biotic stress phenotyping specialist LM Suresh collaborated with Jose Luis Araus and Shawn Kefauver, scientists at the University of Barcelona, Spain, to develop image analysis software that can quantify disease severity, thereby avoiding problems associated with unintentional human bias.

Plant breeders need uniform, or homozygous, lines for selection. With conventional plant breeding this is difficult: no matter how many times you cross a line, a small amount of DNA will remain heterozygous — having two different alleles of a particular gene — and reduce accuracy in line selection.

A technology called doubled haploid allows breeders to develop homozygous lines within two seasons. While this technology has been used in temperate maize breeding programs since the 1990s, it was not available for tropical environments until 10 years ago. In 2013, thanks to joint work with Kenyan partners at the CIMMYT Doubled Haploid facility in Kiboko, this technology was made available to African breeding programs. Now Vijay Chaikam, a CIMMYT doubled haploid specialist based in Kenya, is working towards reducing the cost of this technology as well.

The efforts begun by the STMA research team is now continuing under the Accelerating Genetic Gains in Maize and Wheat for Improved Livelihoods (AGG) project. As this work is carried forward, the next crucial step is ensuring that the next generation of African maize breeders have access to these technologies and tools.

“Improving national breeding programs will really drive success in raising maize yields in the stress prone environments faced by many farmers in our target countries,” says Mike Olsen, CIMMYT’s upstream trait pipeline coordinator. Under AGG, in collaboration with the CGIAR Excellence in Breeding Program, these tools will be scaled out.

CIMMYT researcher Demewoz Negera at the Ambo Research Center in Ethiopia. (Photo: Peter Lowe/CIMMYT)

Collaborating to accelerate genetic gains in maize and wheat

Written by Marta Millere on September 11, 2020. Posted in Features.

Stakeholders in the Accelerating Genetic Gains in Maize and Wheat for Improved Livelihoods (AGG) project have pledged to strengthen efforts to deliver desirable stress tolerant, nutritious and high-yielding maize and wheat varieties to smallholder farmers in a much shorter time. The alliance, comprising funders, national agricultural research systems (NARS), private seed companies, non-governmental organizations, the International Maize and Wheat Improvement Center (CIMMYT) and, for the maize component the International Institute for Tropical Agriculture (IITA), made these assurances during virtual events held in July and August 2020, marking the inception of the 5-year AGG project.

The initiative seeks to fast-track the development of higher-yielding, climate resilient, demand-driven, gender-responsive and nutritious seed varieties for maize and wheat, two of the world’s most important staple crops. The project is funded by the Bill & Melinda Gates Foundation, the Foreign, Commonwealth & Development Office (FCDO), the U.S. Agency for International Development (USAID), and the Foundation for Food and Agriculture Research (FFAR).

Tackling current and emerging threats

Jeff Rosichan, scientific program director of the Foundation for Food and Agricultural Research (FFAR), acknowledged the significant and ambitious aim of the project in tackling the challenges facing maize and wheat currently and in the future. “We are seeing the emergence of new pests and pathogens and viral diseases like never before. A lot of the work of this project is going to help us to tackle such challenges and to be better prepared to tackle emerging threats,” he said.

AGG builds on gains made in previous initiatives including Drought Tolerant Maize for Africa (DTMA), Improved Maize for African Soils (IMAS), Water Efficient Maize for Africa (WEMA), Stress Tolerant Maize for Africa (STMA) and Delivering Genetic Gain in Wheat (DGGW), with support from partners in 17 target countries in sub-Saharan Africa (SSA) and South Asia.

Hailu Wordofa, agricultural technology specialist at the USAID Bureau for Resilience and Food Security, underscored his expectation for CIMMYT’s global breeding program to use optimal breeding approaches and develop strong collaborative relationships with NARS partners, “from the development of product profiles to breeding, field trials and line advancement.”

Similarly, Gary Atlin, senior program officer at the Bill & Melinda Gates Foundation lauded the move toward stronger partnerships and greater emphasis on the CIMMYT and IITA breeding programs. “The technical capacity of partners has increased through the years. It is prudent to ensure that national partnerships continue. It is always a challenging environment, this time multiplied by the COVID-19 crisis, but through this collaboration, there is a greater scope to strengthen such partnerships even more,” he said.

Anne Wangui, Maize Seed Health Technician, demonstrates how to test maize plants for maize dwarf mosaic virus (MDMV). (Photo: Joshua Masinde/CIMMYT)

Symbiotic partnerships with great impact

“From the NARS perspective, we are committed to doing our part as primary partners to deliver the right seed to the farmers,” said Godfrey Asea, director of the National Crops Resources Research Institute at the National Agriculture Research Organization (NARO), Uganda. “We see an opportunity to review and to use a lot of previous historical data, both in-country and regionally and to continue making improved decisions. We also reiterate our commitment and support to continuously make improvement plans in our breeding programs.”

Martin Kropff, director general of CIMMYT, recognized the tremendous impact arising from the longstanding cooperation between CIMMYT’s maize and wheat programs and national programs in countries where CIMMYT works. “A wheat study in Ethiopia showed that 90% of all the wheat grown in the country is CIMMYT-related, while an impact study for the maize program shows that 50% of the maize varieties in Africa are CIMMYT-derived. We are very proud of that – not for ourselves but for the people that we work for, the hundreds of millions of poor people and smallholder farmers who really rely on wheat and maize for their living and for their incomes,” he said.

Founder and Chief Executive Officer of East Africa-based Western Seed Company Saleem Esmail expressed optimism at the opportunities the project offers to improve livelihoods of beneficiaries. “I believe we can do this by sharing experiences and by leveraging on the impacts that this project is going to bring, from new technologies to new science approaches, particularly those that help save costs of seed production.”

He, however, observed that while the target of fast-tracking varietal turnover was great, it was a tough call, too, “because farmers are very risk averse and to change their habits requires a great deal of effort.”

On his part, director of Crop Research at the Oromia Agricultural Research Institute (OARI) in Ethiopia Tesfaye Letta revealed that from collaborative research work undertaken with CIMMYT, the institute has had access to better-quality varieties especially for wheat (bread and durum). These have helped millions of farmers to improve their productivity even as Ethiopia aims for wheat self-sufficiency by expanding wheat production under irrigation.

“We expect more support, from identifying wheat germplasm suitable for irrigation, developing disease resistant varieties and multiplying a sufficient quantity of early generation seed, to applying appropriate agronomic practices for yield improvement and organizing exposure field visits for farmers and experts,” he said.

Challenges and opportunities in a time of crisis

Alan Tollervey, head of agriculture research at Foreign, Commonwealth and Development Office (FCDO) and the UK representative to the CGIAR System Council, emphasized the need for continued investment in agricultural research to build a resilient food system that can cope with the demands and pressures of the coming decades. This way, organizations such as CIMMYT and its partners can adequately deliver products that are relevant not only to the immediate demands of poor farmers in developing countries – and the global demand for food generally – but also to address foreseen threats.

“We are at a time of intense pressure on budgets, and that is when projects are most successful, most relevant to the objectives of any organization, and most able to demonstrate a track record of delivery. CIMMYT has a long track history of being able to respond to rapidly emerging threats,” he said.

Felister Makini, the deputy director general for crops at the Kenya Agricultural Research Organization (KALRO) lauded the fact that AGG not only brings together maize and wheat breeding and optimization tools and technologies, but also considers gender and socioeconomic insights, “which will be crucial to our envisioned strategy to achieve socioeconomic change.”

Zambia Agriculture Research Organization (ZARI) maize breeder Mwansa Kabamba noted that the inclusion of extension workers will help to get buy-in from farmers especially as far as helping with adoption of the improved varieties is concerned.

In its lifecycle, the AGG project aims to reduce the breeding cycles for both maize and wheat from 5-7 years currently to 3-4 years. By 2024, at least 150,000 metric tons of certified maize seed is expected to be produced, adopted by 10 million households, planted on 6 million hectares and benefit 64 million people. It also seeks to serve over 30 million households engaged in wheat farming the target countries.

Cover photo: CIMMYT researcher Demewoz Negera at the Ambo Research Center in Ethiopia. (Photo: Peter Lowe/CIMMYT)

Wheat fields at the Campo Experimental Norman E. Borlaug (CENEB) near Ciudad Obregón, Sonora, Mexico. (Photo: M. Ellis/CIMMYT)

“Better, faster, equitable, sustainable” – wheat research community partners join to kick off new breeding project

Written by Marcia MacNeil on July 29, 2020. Posted in News. 1 Comment on “Better, faster, equitable, sustainable” – wheat research community partners join to kick off new breeding project

More than 100 scientists, crop breeders, researchers, and representatives from funding and national government agencies gathered virtually to initiate the wheat component of a groundbreaking and ambitious collaborative new crop breeding project led by the International Maize and Wheat Improvement Center (CIMMYT).

The new project, Accelerating Genetic Gains in Maize and Wheat for Improved Livelihoods, or AGG, brings together partners in the global science community and in national agricultural research and extension systems to accelerate the development of higher-yielding varieties of maize and wheat — two of the world’s most important staple crops.

Funded by the Bill & Melinda Gates Foundation, the U.K. Department for International Development (DFID), the U.S. Agency for International Development (USAID), and the Foundation for Food and Agriculture Research (FFAR), the project specifically focuses on supporting smallholder farmers in low- and middle-income countries. The international team uses innovative methods — such as rapid cycling and molecular breeding approaches — that improve breeding efficiency and precision to produce varieties that are climate-resilient, pest and disease resistant and highly nutritious, targeted to farmers’ specific needs.

The wheat component of AGG builds on breeding and variety adoption work that has its roots with Norman Borlaug’s Nobel Prize winning work developing high yielding and disease resistance dwarf wheat more than 50 years ago. Most recently, AGG builds on Delivering Genetic Gain in Wheat (DGGW), a 4-year project led by Cornell University, which ends this year.

“AGG challenges us to build on this foundation and make it better, faster, equitable and sustainable,” said CIMMYT Interim Deputy Director for Research Kevin Pixley.

At the virtual gathering on July 17, donors and partner representatives from target countries in South Asia joined CIMMYT scientists to describe both the technical objectives of the project and its overall significance.

“This program is probably the world’s single most impactful plant breeding program. Its products are used throughout the world on many millions of hectares,” said Gary Atlin from the Bill & Melinda Gates Foundation. “The AGG project moves this work even farther, with an emphasis on constant technological improvement and an explicit focus on improved capacity and poverty alleviation.”

Alan Tollervey from DFID spoke about the significance of the project in demonstrating the relevance and impact of wheat research.

“The AGG project helps build a case for funding wheat research based on wheat’s future,” he said.

Nora Lapitan from the USAID Bureau for Resilience and Food Security listed the high expectations AGG brings: increased genetic gains, variety replacement, optimal breeding approaches, and strong collaboration with national agricultural research systems in partner countries.

India’s farmers feed millions of people. (Photo: Dakshinamurthy Vedachalam)

Reconnecting with trusted partners

The virtual meeting allowed agricultural scientists and wheat breeding experts from AGG target countries in South Asia, many of whom have been working collaboratively with CIMMYT for years, to reconnect and learn how the AGG project both challenges them to a new level of collaboration and supports their national wheat production ambitions.

“With wheat blast and wheat rust problems evolving in Bangladesh, we welcome the partnership with international partners, especially CIMMYT and the funders to help us overcome these challenges,” said Director General of the Bangladesh Wheat and Maize Research Institute Md. Israil Hossain.

Director of the Indian Institute for Wheat and Barley Research Gyanendra P. Singh praised CIMMYT’s role in developing better wheat varieties for farmers in India.

“Most of the recent varieties which have been developed and released by India are recommended for cultivation on over 20 million hectares. They are not only stress tolerant and high yielding but also fortified with nutritional qualities. I appreciate CIMMYT’s support on this,” he said.

Executive Director of the National Agricultural Research Council of Nepal Deepak K. Bhandari said he was impressed with the variety of activities of the project, which would be integral to the development of Nepal’s wheat program.

“Nepal envisions increased wheat productivity from 2.84 to 3.5 tons per hectare within five years. I hope this project will help us to achieve this goal. Fast tracking the replacement of seed to more recent varieties will certainly improve productivity and resilience of the wheat sector,” he said.

The National Wheat Coordinator at the National Agricultural Research Center of Pakistan, Atiq Ur-Rehman, told attendees that his government had recently launched a “mega project” to reduce poverty and hunger and to respond to climate change through sustainable intensification. He noted that the support of AGG would help the country increase its capacity in “vertical production” of wheat through speed breeding. “AGG will help us save 3 to 4 years” in breeding time,” he said.

For CIMMYT Global Wheat Program Director Hans Braun, the gathering was personal as well as professional.

“I have met many of you over the last decades,” he told attendees, mentioning his first CIMMYT trip to see wheat programs in India in 1985. “Together we have achieved a lot — wheat self-sufficiency for South Asia has been secured now for 50 years. This would not be possible without your close collaboration, your trust and your willingness to share germplasm and information, and I hope this will stay. “

Braun pointed out that in this project, many national partners will gain the tools and capacity to implement their own state of the art breeding strategies such as genomic selection.

“We are at the beginning of a new era in breeding,” Braun noted. “We are also initiating a new era of collaboration.”

The wheat component of AGG serves more than 30 million wheat farming households in Bangladesh, Ethiopia, India, Kenya, Nepal and Pakistan. A separate inception meeting for stakeholders in sub-Saharan Africa is planned for next month.