Dave Hodson, International Maize and Wheat Improvement Center (CIMMYT) senior scientist delivered a large-scale overview of the current global wheat rust situation and the state of disease surveillance systems. He underscored the importance of comprehensive early warning systems and promising new detection tools that help to raise awareness and improve control. A new assessment of the early warning system for rust In Ethiopia showed a real impact on farmers’ interest, awareness, and farming practices to control the disease, as well as high-level policy changes.
Alison Bentley, CIMMYT Global Wheat Program director, described cutting-edge tools and methods by CIMMYT and, in particular, the Accelerating Genetic Gains in Maize and Wheat for Improved Livelihoods (AGG) project to increase wheat productivity in the face of changing climates. In addition to the new approaches on the supply side, she argued, we also need increased research on the demand side to better understand why farmers will choose a new variety, the role of markets and gender, and how we can scale up these systems. Bentley emphasized the criticality of supporting public and private sector efforts to get more improved germplasm into farmers’ fields in less time.
Philomin Juliana, CIMMYT Global Wheat Program associate scientist highlighted the pivotal role that data plays in breeding decisions and line advancements in CIMMYT’s wheat breeding program. This has been facilitated by improvements in how data sets, like genomic estimated breeding values (GEBVs), are shared with breeders. “CIMMYT has adopted a holistic, data-driven selection approach” that leverages phenotypic data, genomic-estimated breeding values (GEBVs) and selection indices, Juliana explained.
Health has certainly been in the spotlight over the past year. And how could it not be?
The ongoing COVID-19 pandemic has thrown into sharp relief the fact that many groups across the world struggle to make ends meet with little daily income, have poorer housing conditions and education, fewer employment opportunities, and have little or no access to safe environments, clean water and air, food security and health services.
In light of this, the World Health Organization (WHO) is calling on leaders worldwide to ensure that everyone has living and working conditions that are conducive to good health. For many the focus will, understandably, be on access to quality health care services. But there are myriad other factors that influence our ability to lead healthy lives — from how we care for our soil, to what we eat and the air we breathe.
Joining this year’s World Health Day campaign, the International Maize and Wheat Improvement Center (CIMMYT) is highlighting five areas where it pays to think about health, and the solutions we can use to help build a healthier world for everyone.
Douglas Mungai holds up soil on his farm in Murang’a county, Kenya. (Photo: Robert Neptune/TNC)
Robust germplasm
How do we ensure that germplasm reserves are not potential vectors of pest and disease transmission? The second instalment in the CGIAR International Year of Plant Health Webinar Series tackles the often-overlooked issue of germplasm health.
A CIMMYT gene bank worker photographs maize accessions for the database for future reference. (Photo: Alfonso Cortés/CIMMYT)
Farmer Roba Shubisha harvests an improved maize variety in Yubo village, Wondo Genet, Ethiopia. (Photo: Peter Lowe/CIMMYT)
With almost all CGIAR centers represented in Addis Ababa, Ethiopia is considered to be a hub for CGIAR research, and the organization has been a long-term partner to the Ethiopian government when it comes to agriculture. The partnership between CGIAR and the national partners is said to be an exemplary one, with CGIAR serving as the source of new technologies and innovations and national partners contextualizing these products within their own country context. This is believed to have brought impacts that serve the people on the ground.
A new report by CGIAR’s Standing Panel on Impact Assessment (SPIA) indicates that CGIAR innovations have reached between 4.1 and 11 million Ethiopian households. The report — which assesses 52 agricultural innovations and 26 claims of policy influence — documents the reach of CGIAR-related agricultural innovations across the core domains of CGIAR research activity: animal agriculture; crop germplasm improvement; natural resource management; and policy research.
The study compiles comprehensive information on the past two decades of CGIAR research activities in Ethiopia. Using information from interviews with CGIAR research leaders, scientists, government officials, published studies and project documents, this ‘stocktaking’ exercise was used to identify the innovations which are potentially disseminated at scale. The study also employs novel data collection protocols and methods like visual aid protocols for identification of natural resource management innovations or DNA fingerprinting for crop variety identification for barley, maize and sorghum.
The study results show that although many innovations are being adopted by some farmers, only a few are reaching large numbers of households. The three innovations with the largest reach are soil and water conservation practices, improved maize varieties and crossbred poultry. The study also found out that there are synergies between innovations where households adopt two or more. For instance, a household which adopts CGIAR maize varieties is likely to also adopt recommended natural resource management practices.
This, according to the study, is the result of different categories of CGIAR research efforts — natural resource management and policy, crop breeding and livestock research, respectively. The scaling of these innovations can also be linked to supportive government policies, which in turn have been influenced by policy research, as indicated in the report.
A farmer walks through a maize field in Toga village, Hawassa, Ethiopia. (Photo: Peter Lowe/CIMMYT)
CIMMYT’s footprint
The International Maize and Wheat Improvement Center (CIMMYT) has maintained a presence in Ethiopia for over 30 years and is committed to supporting long-term agricultural development in the country. As part of this effort, CIMMYT has contributed to an increase in maize and wheat production in Ethiopia, working with national partners to test and release improved varieties.
The maize breeding program started in 1988 through CIMMYT and EIAR collaboration and in 1993 BH-660 was released — the first hybrid maize variety derived from CIMMYT germplasm. According to the report, specific maize traits were researched through the Drought Tolerant Maize for Africa (DTMA) and Drought Tolerant Maize for Africa Seed Scaling (DTMASS) projects, and since 2012 the Nutritious Maize for Ethiopia (NuME) project has aimed to develop varieties with higher protein content. Overall, 54 maize varieties have been released in Ethiopia since 1990, and 34 of these are thought to contain CIMMYT-related germplasm. It is also noted that, in the past 20 years ten drought-tolerant varieties and eight quality protein maize (QPM) varieties have been released.
In terms of geographical spread, the study highlights that improved maize varieties derived from CGIAR germplasm were highly adopted in the regions of Harar and Dire Dawa, which account for 81% of adopters overall. Adoption rates were also high in Tigray (79.3% of households), Amhara and the Southern Nations, Nationalities, and Peoples’ Region (63% of households), and Oromia (58.4% of households).
The other important crop in Ethiopia is wheat, which is grown by up to 4.8 million farmers in the country, according to the 2019 Central Statistics Authority (CSA) report. The SPIA document indicates that CGIAR innovations have played great role in the release and uptake of improved wheat varieties. The work of the CGIAR Research Program on Wheat (WHEAT), for instance, has resulted in the release of eight rust-resistant varieties derived from CIMMYT germplasm that are still under production. Of the 133 varieties released since 1974, CIMMYT and the International Center for Agricultural Research in the Dry Areas (ICARDA) played a role in developing at least 80.
The report concludes that agricultural research carried out by CGIAR scientists and their national partners generates many new ideas for innovations that might help address pressing policy concerns. CGIAR’s contribution to Ethiopia’s agricultural development is complex and wide-ranging, and while some aspects cannot be accurately captured by survey data, this new source of adoption and diffusion data helps identify the scale and scope of CGIAR’s reach in Ethiopia.
The Standing Panel on Impact Assessment (SPIA) is an external, impartial panel of experts in impact assessment appointed by the System Council and accountable to it. SPIA is responsible for providing rigorous, evidence-based, and independent strategic advice to the broader CGIAR System on efficient and effective impact assessment methods and practices, including those measuring impacts beyond contributions to science and economic performance, and on innovative ways to improve knowledge and capacity on how research contributes to development outcomes
Maize is more than a crop in Mexico. In many cases, it connects families with their past. Landraces are maize varieties that have been cultivated and subjected to selection by farmers for generations, retaining a distinct identity and lacking formal crop improvement. They provide the basis of Mexico’s maize diversity.
Back in 1966-67, researcher Ángel Kato from the International Maize and Wheat Improvement Center (CIMMYT) collected 93 maize landraces samples from 66 families in Mexico’s state of Morelos. These seeds were safeguarded in CIMMYT’s Germplasm Bank, which today stores 28,000 samples of maize and its wild relatives from 88 countries.
50 years later, doctoral candidate Denisse McLean-Rodriguez, from the Sant’Anna School of Advanced Studies in Italy, and researchers from CIMMYT started a new study to trace the conservation and abandonment of maize landraces over the years.
The study shows that landrace abandonment is common when farming passes from one generation to the next. Older farmers were attached to their landraces and continued cultivating them, even in the face of pressing reasons to change or replace them. When the younger generations take over farm management, these landraces are often abandoned. Nonetheless, young farmers still value the cultural importance of landraces.
Maize landraces can be conserved “in situ” in farmers’ fields and “ex situ” in a protected space such as a germplasm bank or community seed bank. The loss of landraces in farmers’ fields over 50 years emphasizes the importance of ex situ conservation. Traits found in landraces can be incorporated into new varieties to address some of the world’s most pressing agriculture challenges like changing climates, emerging pests and disease, and malnutrition.
This research was supported by the CGIAR Research Program on Maize (MAIZE), the Sant’Anna School of Advanced Studies, Wageningen University and the Global Crop Diversity Trust.
Whenever seed is transferred between countries, continents or regions there is an inherent risk that new plant pathogens could spread to previously non-infested areas — with potentially devastating consequences. FAO estimates that these pathogens are responsible for the loss of up to 40% of global food crops, and for trade losses in agricultural products exceeding $220 billion each year.
With old and new pests and diseases causing devastation across the world, it is becoming increasingly important to consider plant health. This is especially true at the International Maize and Wheat Improvement Center (CIMMYT), an organization which processes and distributes enormous quantities of seed each year and in 2019 alone sent over 10,000 tons to more than 100 partners in Africa, the Americas, Asia and Europe.
Amos Alakonya joined CIMMYT in July 2019, and as head of the organization’s Seed Health Unit he is acutely aware of the need to mitigate risk throughout the seed production value chain.
In the lead up to this year’s International Phytosanitary Awareness Week, the plant pathologist sits down to discuss pests, screening procedures, and explain why everyone should be talking about seed health.
Amos Alakonya, head of CIMMYT’s Seed Health unit. (Photo: Eleusis Llanderal/CIMMYT)
Can you start by telling us about the CGIAR Germplasm Health Unit consortium and what it does?
Within CGIAR we have a cluster called Genebank Platform whose main function is to support CGIAR efforts in conservation and distribution of germplasm. Ten CGIAR Centers have germplasm banks that work closely with germplasm health units to ensure that they only distribute plant materials free from pests and diseases.
What is the procedure for introducing seed at CIMMYT?
At CIMMYT, researchers must follow the correct procedure when bringing in seed. Once someone has identified the need to bring in seed, contacted a supplier and agreed on the genotypes and amount required, the responsibility is transferred to the Seed Health Unit. We take care of communication with the seed supplier and provide support in acquisition of the necessary phytosanitary documentation that will ensure compliance with host country rules.
For instance, we will process and provide a plant import permit allowing us to bring in the seed while also stipulating the conditions it must meet before entry into Mexico. This document is used as the standard guide by the authorities in the supplier country, commonly referred to us National Plant Protection Organization (NPPO). The NPPO will then perform a pre-shipment verification and issue a phytosanitary certificate if the seed meets the standards stated in the import permit.
Because we distribute our materials as public goods, we ensure that all seed sent out or received can be used and distributed without restrictions from the supplier or the recipient. This is achieved by the signing of a standard material transfer agreement that complies with International treaty on Plant Genetic Resources for Food and Agriculture. This is done through CIMMYT’s legal unit.
Petri dishes and a microscope in Amos Alakonya’s lab. (Photo: Eleusis Llanderal/CIMMYT)
Once we have received all the necessary documents, materials are cleared through customs and delivered to the lab, where we begin our analysis. The first thing we do is assess the material visually and confirm there is no discoloration and no foreign material like soil or seeds from other species. At the next stage, we set up several assays to detect fungi, bacteria and viruses. We only release seed to scientists or allow distribution after we’ve confirmed they are free from injurious pathogens. Overall, this process takes between 25 and 40 days, so scientists must plan ahead to avoid any inconvenience.
That sounds like a complex process. Do you face any challenges along the way?
There are several challenges but we work around them. One of the biggest ones is meeting up with time expectations. For example, every scientist wants to make sure that they’re on track, but sometimes the seed takes longer than expected to arrive or the documentation gets misplaced which means the seed cannot be released from customs in time.
Even after a delay, the seed has to still pass through the standard health testing procedure. Sometimes we find that the supplier’s NPPO hasn’t carried out the right tests, so we bring in seed that turns out to be non-compliant and may end up being destroyed as a result. However, we only recommend seed destruction in cases where we can’t mitigate.
That’s why it’s crucial that everyone — at all stages of the seed production value chain — is aware of the risks and appropriate mitigation processes. These include checking seed before planting, regular field inspections, and observing field hygiene and spraying regimes.
The theme for this year’s event focuses on transboundary threats to plant health. Are there any emerging ones that you’re concerned about?
Currently there are three main concerns. The first is Maize Lethal Necrosis. The disease was initially reported in the USA and Peru in 1977, but since 2011 the disease has been invading farms in east and central Africa. Because of this, maize breeders in the region cannot send seed directly to their partners in other regions of the world without going through a quarantine field station in Zimbabwe. This comes with additional costs and time burden to the program.
We’re also very concerned about wheat blast, which is now present in Bangladesh where we have trials and share seed in both directions. We have therefore already put in place screening tools against wheat blast to ensure we do not introduce it into experimental fields in Mexico.
And finally, we have the fall armyworm. This pest is indigenous to South America where it is less ferocious, but ever since it reached Africa around 2016 it has been causing destruction to maize and costing farmers lots of money to control through application of chemicals. This emerging disease really undermines food security efforts.
This is obviously an important topic to raise global awareness about. Why do you think it is so crucial to discuss seed health within CIMMYT internally as well?
Amos Alakonya, head of CIMMYT’s Seed Health unit. (Photo: Eleusis Llanderal/CIMMYT)
It’s very important that everyone working at CIMMYT, and especially those working with seed, is aware of the potential risks because about 30% of maize and 50% of wheat grown worldwide can be traced to CIMMYT germplasm. And it’s even more important for Mexico because most of our wheat breeding program is based here and it is also the center of origin for maize. With partners in more than 100 countries we have to be extremely vigilant. If anything goes wrong here, many countries will be at risk.
Ultimately, we want people to be aware of the important role they play in ensuring phytosanitary compliance because prevention is better than cure. We would like to envisage a situation where everybody in CIMMYT is aware of the mitigation processes that have been put in place to ensure safe seed exchanges.
Will you continue working to raise awareness beyond this year’s event?
Yes. In December 2018, the United Nations declared 2020 the International Year of Plant Health. Everybody will be encouraged to take this opportunity to inform people about the importance of seed health, especially as it relates to food security, environmental conservation and economic empowerment.
It’s exciting because this event only happens every 30 to 50 years, so this is really a once in a lifetime opportunity to showcase the work we do every day, both as a unit and in collaboration with our global partners.
Cover photo:
A mixture of maize seeds seen in close-up. (Photo: Xochiquetzal Fonseca/CIMMYT)
The Frank N. Meyer Medal for Plant Genetic Resources. (Photo: Kevin Pixley/CIMMYT)
Thomas Payne, head of the Wheat Germplasm Bank at the International Maize and Wheat Improvement Center (CIMMYT), was awarded the Frank N. Meyer Medal for Plant Genetic Resources this morning at the annual meeting of the American Society of Agronomy, the Crop Science Society of America, and the Soil Science Society of America, held in San Antonio, Texas.
The Frank N. Meyer Medal recognizes contributions to plant germplasm collection and use, as well as dedication and service to humanity through the collection, evaluation or conservation of earth’s genetic resources. The award was presented by Clare Clarice Coyne, U.S. Department of Agriculture (USDA) research geneticist.
As an award recipient, Payne delivered a lecture that touched on the philosophy, history and culture surrounding plant genetic diversity and its collectors, and CIMMYT’s important role in conserving and sharing crop diversity.
The scientist has focused his career on wheat improvement and conservation. In addition to leading CIMMYT’s Wellhausen-Anderson Wheat Genetic Resources Collection, one of the world’s largest collection of wheat and maize germplasm, he manages the CIMMYT International Wheat Improvement Network. He is the current Chair of the Article 15 Group of CGIAR Genebank Managers, and has served as Secretary to the CIMMYT Board of Trustees. His association with CIMMYT began immediately after obtaining a PhD at the University of Nebraska–Lincoln in 1988, and he has held positions for CIMMYT in Ethiopia, Mexico, Syria, Turkey and Zimbabwe.
Thomas Payne delivers a presentation at the Crop Science Society of America’s annual Genetic Resources breakfast, where he received the award. (Photo: Kevin Pixley/CIMMYT)
“CIMMYT is the largest distributor of maize and wheat germplasm worldwide, with materials emanating from its research and breeding programs, as well as held in-trust in the germplasm bank. The Meyer Medal is a reflection of the impact CIMMYT makes in the international research community — and in farmers’ fields throughout the developing world,” Payne said.
Located at CIMMYT headquarters outside Mexico City, the CIMMYT Wheat Germplasm Bank contains nearly 150,000 collections of seed of wheat and related species from more than 100 countries. Collections preserve the diversity of unique native varieties and wild relatives of wheat and are held under long-term storage for the benefit of humanity, in accordance with the 2007 International Treaty on Plant Genetic Resources for Food and Agriculture. The collections are also studied and used as a source of diversity to breed for crucial traits such as heat and drought tolerance, resistance to crop diseases and pests, grain yield productivity, and grain quality. Seed is freely shared on request to researchers, students, and academic and development institutions worldwide.
In his remarks, Payne also highlighted the story of Frank N. Meyer, after whom the award is named. Meyer, an agricultural explorer for the USDA in the 1900s, spent a decade traveling under harsh conditions through China to collect new plant species suitable for production on the United States’s expanding farmland. Among more than 2,500 plants that he introduced to the U.S. — including varieties of soybeans, oats, wild pears, and asparagus — the Meyer lemon was named in his honor. As he pointed out, Meyer worked during a historical period of great scientific discoveries, including those by his contemporaries Marie Curie and the Wright brothers.
Among those attending the ceremony were Payne’s sister, Susan Payne, and CIMMYT colleagues Kevin Pixley, director of Genetic Resources; Denise Costich, head of the CIMMYT Maize Germplasm Bank; and Alexey Morgunov, head of the Turkey-based International Winter Wheat Improvement Program.
The head of CIMMYT’s Global Wheat Program Hans-Joachim Braun and CIMMYT scientist Alexey Morgunov are also receiving honors or awards this week at the annual meeting of the American Society of Agronomy, the Crop Science Society of America, and the Soil Science Society of America. The meeting convenes around 4,000 scientists, professionals, educators, and students to share knowledge and recognition of achievements in the field.
Thomas Payne (right) celebrates the award with his sister Susan Payne (center) and CIMMYT scientist Alexey Morgunov. (Photo: Kevin Pixley/CIMMYT)
Thomas Payne (left) stands for a photo with CIMMYT’s Director of Genetic Resources Kevin Pixley.
Thomas Payne (left) with Head of CIMMYT’s Maize Germplasm Bank Denise Costich. (Photo: Kevin Pixley/CIMMYT)
Maize ears from CIMMYT’s collection, showing a wide variety of colors and shapes. CIMMYT’s germplasm bank contains about 28,000 unique samples of cultivated maize and its wild relatives, teosinte and Tripsacum. These include about 26,000 samples of farmer landraces — traditional, locally-adapted varieties that are rich in diversity. The bank both conserves this diversity and makes it available as a resource for breeding. (Photo: Xochiquetzal Fonseca/CIMMYT)
Imagine walking through a grocery store, doing your weekly shopping. Everything seems normal, but as you pick up a can, there’s no label. There’s nothing to tell you what the product is, and now you can’t reliably choose anything to eat this week.
Now switch gears and imagine a germplasm bank. Without the right labeling on these different varieties, it’s difficult to tell what’s new and what’s already been discovered when working on new research projects.
About nine years ago, the International Maize and Wheat Improvement Center (CIMMYT) started an initiative called the Seeds of Discovery (SeeD). This initiative facilitates easier access to and use of maize and wheat genetic resources.
SeeD achieves impact through five main components: genotyping, phenotyping, software tools, pre-breeding and capacity building.
“One of the aims of Seeds of Discovery was to best characterize germplasm,” says Sarah Hearne, a molecular geneticist and maize lead of SeeD. “At CIMMYT, our international germplasm bank holds in trust one of the largest and most diverse publicly available maize collections in the world.”
However, Hearne says this germplasm bank used to look like a grocery store without any labels or without labels that would allow someone to select a can of value. To combat this, SeeD decided to work on a labeling process for the germplasm bank: the Molecular Maize Atlas.
The Molecular Maize Atlas is an information platform that brings genotypic data resources and associated tools together. This genotypic data provides unifying information across landraces and acts as a common backbone, which other valuable information, like phenotypic data, can be added to.
The new lines are specifically adapted to tropical/subtropical maize production environments in Africa, Asia and Latin America, and are freely available to both public and private sector breeders worldwide.
CML582, one of the 26 new CIMMYT maize lines released by the Center. (Photo: CIMMYT)
CIMMYT is pleased to announce the release of a set of 26 new CIMMYT maize lines (CMLs). These CMLs were developed by the CIMMYT Global Maize Program’s multi-disciplinary teams of scientists at breeding locations in sub-Saharan Africa, Latin America and Asia. These lines are adapted to the tropical/subtropical maize production environments targeted by CIMMYT and partner institutions. CMLs are freely available to both public and private sector breeders worldwide under the standard material transfer agreement (SMTA).
CIMMYT seeks to develop improved maize inbred lines with superior performance and multiple stress tolerance to improve maize productivity for resource-constrained smallholder farmers. To achieve this aim, CMLs are released after intensive evaluation in hybrid combinations under various abiotic and biotic stresses. Suitability as either seed or pollen parent is also thoroughly evaluated.
Release of a CML does not guarantee high combining ability or per se performance in all environments; rather, it indicates that the line is promising or useful as a hybrid component or parent for pedigree breeding for one or more target mega-environments. The descriptions of the lines include heterotic group classification, along with information on their specific combining ability with widely-used CIMMYT lines.
For a summary of the 26 new CMLs, please click here.
Further details on all CMLs, including the pedigrees, are available here.
A limited quantity of seed of the CMLs can be obtained from the CIMMYT Germplasm Bank. To send a request, please contact Denise Costich, Head of the Maize Genetic Resources Center: d.costich@cgiar.org.
For further details, please contact B.M. Prasanna, Director of the CGIAR Research Program MAIZE and Director of CIMMYT’s Global Maize Program: b.m.prasanna@cgiar.org.
For the first time ever, a research team of more than 40 scientists has genetically characterized values of exotics in hexaploid wheat. CIMMYT scientists, together with partners in Demark, India, Mexico, Pakistan, and the UK, used next-generation sequencing and multi-environment phenotyping to study the contribution of exotic genomes to pre-breeding lines. Research required collaborative development, evaluation, and deployment of novel genetic resources to breeding programs addressing food security under climate change scenarios in India, Mexico, and Pakistan.
The team generated large-scale pre-breeding materials, which have been evaluated for important traits such as grain yield, quality, and disease resistance. Pre-breeding and haplotype-based approaches revealed useful genetic footprints of exotic lines in pre-breeding germplasm. Results of the study, recently published in Nature Scientific Reports, show that some DNA from exotic germplasm improved the biotic and abiotic stress tolerances of lines derived from crosses of exotics with CIMMYT’s best elite lines.
The practical successes of large-scale, impact-oriented breeding work will be useful to other wheat breeding programs around the world, and the information generated could be used to boost global wheat productivity.
Sukhwinder Singh, wheat lead on CIMMYT’s SeeD Project, explains that pre-breeding is in-demand and the resources developed through this study can serve as tools to address upcoming challenges in wheat production more efficiently, as desirable alleles from exotics have been mobilized into best elite genetic background. Breeding programs can now use this material to deliver outcomes in shorter timeframes by avoiding the lengthy process of searching for exotics first.
This research was conducted as part of the Seeds of Discovery and MasAgro projects in collaboration with the Borlaug Institute for South Asia, and was made possible by generous support from Mexico’s Department of Agriculture, Livestock, Rural Development, Fisheries and Food (SAGARPA), the Government of Punjab, and the CGIAR Research Program on Wheat.
Check out other recent publications by CIMMYT researchers below:
Applications of machine learning methods to genomic selection in breeding wheat for rust resistance. González-Camacho, J.M., Ornella, L., Perez-Rodriguez, P., Gianola, D., Dreisigacker, S., Crossa, J. In: Plant Genome v. 11, no. 2, art. 170104.
Bayesian functional regression as an alternative statistical analysis of high‑throughput phenotyping data of modern agriculture. Montesinos-López, A., Montesinos-López, O.A., De los Campos, G., Crossa, J., Burgueño, J., Luna-Vázquez, F.J. In: Plant Methods v. 14, art. 46.
Effect of ppd-a1 and ppd-b1 allelic variants on grain number and thousand-kernel weight of durum wheat and their impact on final grain yield. Arjona, J.M., Royo, C., Dreisigacker, S., Ammar, K., Villegas, D. In: Frontiers in Plant Science v. 9, art. 888.
Genomic-enabled prediction accuracies increased by modeling genotype × environment interaction in durum wheat. Sukumaran, S., Jarquín, D., Crossa, J., Reynolds, M.P. In: Plant Genome v. 11, no. 2, art. 170112.
Mexican tropical cream cheese yield using low-fat milk induced by trans-10, cis-12 conjugated linoleic acid: effect of palmitic acid. Granados-Rivera, L.D., Hernández-Mendo, O., Burgueño, J., Gonzalez-Munoz, S.S., Mendoza-Martinez, G.D., Mora-Flores, J.S., Arriaga-Jordan, C.M. In: CyTA-Journal of Food v. 16, no. 1, p. 311-315.
