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Tag: doubled haploids

KALRO research station at Kiboko revamped to accelerate crop breeding

CIMMYT Global Maize Program Director and CGIAR Plant Health Initiative Lead, BM Prasanna cutting a ribbon at the entrance of a new shed housing, marking the commissioning of five new seed drying machines courtesy of the of the Accelerating Genetic Gains (AGG) Project. (Photo: Susan Otieno/CIMMYT)

Kenya Agricultural and Livestock Research Organization (KALRO)’s research station at Kiboko, Kenya, where several partner institutions including the International Maize and Wheat Improvement Center (CIMMYT), conduct significant research activities on crop breeding and seed systems, is now equipped with five new seed drying machines along with a dedicated shed to house these units, a cold room for storing breeding materials, and an additional irrigation dam/reservoir. These infrastructural upgrades are worth approximately US $0.5 million.

During the commissioning of the new facilities on February 7, 2023, CIMMYT Global Maize Program Director, BM Prasanna thanked the donors, Crops to End Hunger (CtEH) Initiative and Accelerated Genetic Gains (AGG) project, that supported the upgrade of the research station, and recognized the strong partnership with KALRO.

“Today is a major milestone for CIMMYT, together with KALRO, hosting this center of excellence for crop breeding. This facility is one of the largest public sector crop breeding facilities in the world, with hundreds of hectares dedicated to crop breeding. These new facilities will enable CIMMYT and KALRO crop breeders to optimize their breeding and seed systems’ work and provide better varieties to the farming communities,” said Prasanna.

Kenya suffered one of its worst droughts ever in 2022, and the newly commissioned facilities will support expedited development of climate-resilient and nutritious crop varieties, including resistance to major diseases and pests.

Visitors at the KALRO research station in Kiboko, Kenya, looking at the newly commissioned cold room storage. (Photo: Susan Otieno/CIMMYT)

Improvements and enhancements

The efficiency of the seed driers capabilities to quickly reduce moisture content in seed from above 30% to 12% in two to three days, reducing the time taken for seed drying and allowing for more than two crop seasons per year in a crop like maize.

The additional water reservoir with a capacity of 16,500 cubic meters will eliminate irrigation emergencies and will also enhance the field research capacity at Kiboko. Reliable irrigation is essential for accelerating breeding cycles.

At the same time, the new cold room can preserve the seeds up to two years, preventing the loss of valuable genetic materials and saving costs associated with frequent regeneration of seeds.

KALRO Director General Eliud Kireger officiating the opening of the cold room storage facility at KALRO research station at Kiboko, Kenya. Looking on is CIMMYT Global Maize Program Director, BM Prasanna. (Photo: Susan Otieno/CIMMYT)

World-class research center

“The Kiboko Research Center is indeed growing into an elite research facility that can serve communities in entire sub-Saharan Africa through a pipeline of improved varieties, not only for maize but in other important crops. This will not only improve climate resilience and nutrition, but will contribute to enhanced food and income security for several million smallholder farmers,” said Prasanna.

KALRO Director General Eliud Kireger appreciated the establishment of the new facilities and thanked CIMMYT and its partners for their support.

“Today is a very important day for us because we are launching new and improved facilities for research to support breeding work and quality seed production. This research station is in Makueni County, a very dry area yet important place for research because there is adequate space, especially for breeding,” said Kireger. “We are significantly improving the infrastructure at Kiboko to produce and deliver better seed to our farmers.”

For more than three decades, CIMMYT has conducted research trials at the Kiboko Research Station, focusing on drought tolerance, nitrogen use efficiency, and resistance to pests and diseases, such as fall armyworm and stem borer. The maize Double Haploid (DH) facility established in 2013 at Kiboko, with the support of the Bill & Melinda Gates Foundation, offers DH line production service for organizations throughout Africa, and is key to increasing genetic gains in maize breeding.

Government of Zimbabwe recognizes CIMMYT for beneficial collaborations

Dr Dumisani Kutwayo (second left) receives state of art Maize Lethal Necrosis test kits from Dr Wegary Dagne (second from right). (Photo: Tawanda Hove/CIMMYT)

The best results in combating pests and diseases exacerbated by climate change and protecting agricultural food systems originate from strategic partnerships between national governments and international research organizations. Such a synergy between Zimbabwe’s Department of Research and Specialist Services (DRSS) and the International Maize and Wheat Improvement Center (CIMMYT) was recognized for its effectiveness at an event hosted by Zimbabwe Plant Quarantine Services on January 9, 2023.

“The mandate of ensuring that Zimbabwe is protected from plant diseases and invasive pests is one which cannot be attained by government alone, but together with partners such as CIMMYT,” said Dumisani Kutywayo, Chief Director of DRSS.

Dagne Wegary Gissa, CIMMYT senior scientist in maize breeding, presented Kutywayo with the latest advanced PCR testing kits for detecting maize lethal necrosis. “We are committed to ensuring that we support Zimbabwe with improved maize and wheat varieties but also with rapid disease detection,” said Gissa.

Kutywayo and senior directors were given a tour of the plant quarantine services station, where they observed where all introduced maize seed is quarantined and tested before being incorporated into the local seed systems. Tanyaradzwa Sengwe, a seed health and quality expert, summarized the quarantine procedures and explained how the day-to-day operations between the two institutes are being implemented. This involves the management of imported seed, protocols of seed management and biosafety measures for the quarantine facility.

Government officials take part in a field visit of the quarantine facility set up by CIMMYT in Mazowe, Zimbabwe. (Photo: Tawanda Hove/CIMMYT)

Expanding partnerships

Zimbabwe can now accelerate its crop improvement programs, Gissa indicated, because CIMMYT has provided the government access to doubled haploid (DH) technology. This technology significantly shortens the breeding cycle from seven years to approximately 3-4 years. DH technology has become an integral part of many commercial maize breeding programs, as DH lines offer several economic, logistic and genetic benefits over conventional inbred lines. Further, new advances in DH technology continue to improve the efficiency of DH line development and fuel its increased adoption in breeding programs worldwide.

CIMMYT-Zimbabwe has facilitated access for Zimbabwe’s maize breeding program to a CIMMYT DH facility in Kenya. Busiso Mavankeni, the head of the Crop Breeding Institute, related how it was very expensive for governments of developing countries to keep up with the latest breeding technology trends and so collaborating with CIMMYT is helping Zimbabwe. “Having access to the DH facility has been a great boon to our breeding program,” said Mavankeni.

CIMMYT and Zimbabwe are also engaged in capacity building exercises; involving training sessions across a variety of food system frameworks. Nhamo Mudada, Head of Plant Quarantine Services, acknowledged the multiple trainings ranging from disease identification and prevention systems to entomology related concepts. “Our technical capabilities have increased significantly, and we strongly attribute this to CIMMYT’s knowledge sharing mandate,” Mudada said.

“This sustainability is enabled by ensuring that our systems can screen genetic materials coming into the country and detect diseases which may be foreign to the agroecological region. CIMMYT has, over the years, supported the government not only from a financial perspective but also from a technical capacity perspective.

“Having reliable partners such as CIMMYT who generously invest in government priorities helps our country to be well positioned against threats to our food security,” said Kutywayo, “The key for creating and maintaining sustainable innovation is for development partners like CIMMYT to work within existing national frameworks,” said Kutywayo. “As the adverse effects of climate change intensify, such strategic partnerships are the only way to establish appropriate responses.”

“Our goal is to serve as critical partners for Zimbabwe’s agrifood programs. We have dedicated ourselves to be a long-term partners and will provide as much support as we can to ensure Zimbabwe’s food security,” Gissa said.

State-of-the-art maize doubled haploid facility inaugurated in India

Main building of CIMMYT’s maize doubled haploid facility in Kunigal, Karnataka state, India. (Photo: CIMMYT)
Main building of CIMMYT’s maize doubled haploid facility in Kunigal, Karnataka state, India. (Photo: CIMMYT)

On December 3, 2021, the International Maize and Wheat Improvement Center (CIMMYT) and its partners inaugurated a state-of-the-art maize doubled haploid (DH) facility in Kunigal, in India’s Karnataka state. The facility was established by CIMMYT in partnership with the University of Agricultural Sciences, Bangalore (UAS Bangalore), with financial support from the CGIAR Research Program on Maize (MAIZE).

It is the first public sector facility of its kind in Asia, fulfilling a very important need for maize breeding programs in the region. The facility, operated by CIMMYT, will provide DH production services for CIMMYT’s and UAS Bangalore’s breeding programs, as well as for national agricultural research institutions and small- and medium-sized seed companies engaged in maize breeding across tropical Asia. This is expected to result in accelerated development and deployment of a greater number of elite, climate-resilient and nutritionally-enriched maize hybrids in tropical Asia.

DH technology has the potential to enhance genetic gains and breeding efficiency, especially in combination with other modern tools and technologies, such as molecular markers and genomic selection. The facility occupies 12 acres of land at the Agricultural Research Station in Kunigal, in southwestern India. It is expected to produce at least 25,000-30,000 maize DH lines per year.

For more information, and to request these services, visit CIMMYT’s Maize Doubled Haploid Technology website.

R.S. Paroda (center) cuts the ribbon to inaugurate the maize doubled haploid facility in Kunigal, Karnataka state, India. He is flanked by S. Rajendra Prasad (left), vice chancellor of UAS Bangalore and B.M. Prasanna (right), director of CIMMYT’s Global Maize Program and the CGIAR Research Program on Maize. (Photo: CIMMYT)
R.S. Paroda (center) cuts the ribbon to inaugurate the maize doubled haploid facility in Kunigal, Karnataka state, India. He is flanked by S. Rajendra Prasad (left), vice chancellor of UAS Bangalore and B.M. Prasanna (right), director of CIMMYT’s Global Maize Program and the CGIAR Research Program on Maize. (Photo: CIMMYT)

Fast-track maize breeding in Asia

R.S. Paroda, who is a Padma Bhushan awardee in India and the chairman of the Trust for Advancement of Agricultural Sciences (TAAS) in New Delhi, thanked CIMMYT for its role in developing the facility. “The maize DH facility will revolutionize hybrid maize programs in both the public and private sectors in Asia, enabling fast-tracked development of climate-resilient and genetically diverse maize hybrids suitable for the rainfed maize-growing areas.”

S. Rajendra Prasad, vice chancellor of UAS Bangalore, appreciated the partnership between his institution and CIMMYT. “The facility will create opportunities to modernize maize breeding programs in India, besides serving as an educational and training hub for young students at the University,” he said. Members of UAS Bangalore Board of Management also participated in the formal opening of the facility.

B.M. Prasanna, director of CIMMYT’s Global Maize Program and the CGIAR Research Program on Maize (MAIZE), spearheaded the process of establishing this important breeding facility. “Along with similar maize DH facilities in Mexico and Kenya, which respectively serve Latin America and Africa, this third facility for Asia rounds up CIMMYT’s commitment to strengthen tropical maize breeding programs across the globe,” he explained.

Bram Govaerts, CIMMYT’s director general, participated through a recorded video message.

Attending the ceremony were also 150 post-graduate students, faculty from UAS Bangalore, researchers from UAS Raichur and the Indian Institute of Maize Research, CIMMYT maize scientists, and private-sector members of the International Maize Improvement Consortium for Asia (IMIC-Asia).

R.S. Paroda, chairman of the Trust for Advancement of Agricultural Sciences (TAAS) in New Delhi, unveils the inauguration plaque for the maize doubled haploid facility in Kunigal, Karnataka state, India. (Photo: CIMMYT)
R.S. Paroda, chairman of the Trust for Advancement of Agricultural Sciences (TAAS) in New Delhi, unveils the inauguration plaque for the maize doubled haploid facility in Kunigal, Karnataka state, India. (Photo: CIMMYT)

Collaboration networks

A technical workshop titled “Transforming India’s Agriculture and Modernizing Maize Breeding Programs” was held the same day. The workshop featured talks by Paroda on the role of youth in Indian agriculture, Prasanna on modernizing maize breeding and enhancing genetic gain, CIMMYT scientist Vijay Chaikam on maize doubled haploid technology, and CIMMYT breeder Sudha Nair on genomic technologies for maize improvement.

IMIC-Asia held a General Body Meeting soon after the technical workshop, at which B.S. Vivek, maize breeder at CIMMYT, introduced the framework for the third phase of IMIC-Asia. Participants included representatives of the Indian Institute of Maize Research, the All-India Coordinated Maize Improvement Program, and private seed companies with membership in the consortium. Meeting participants expressed a keen interest in utilizing the new doubled haploid facility’s services.

New publications: Doubled haploids in maize — development, deployment and challenges

Haploids  which are produced naturally in maize  were first identified in the crop about a century ago. Today they are used widely in different breeding programs, particularly in the development of doubled haploids, which are highly uniform, genetically pure and stable. Doubled-haploid technology has simplified logistics to make the maize breeding process more efficient and intuitive, facilitated studies at the molecular and genomic level, and increased genetic gains in different breeding programs.  

In a recent review article, scientists from the International Maize and Wheat Improvement Center (CIMMYT) examine strategies for haploid induction and identification, chromosome doubling and production of doubled haploid seed through self-fertilization. They also discuss the potential applications and key challenges linked with doubled haploid technology in maize, and suggest future research directions for people involved in fast-track maize breeding, the seed industry, and academia.  

Extensive studies of haploids and doubled haploids have increased our understanding of the genetic basis and mechanisms involved in haploid induction, the factors that affect haploid induction, different markers to identify putative haploids, and different chemicals agents that can be used for chromosome doubling.  

The technology is useful because the resulting plants are free from different social issues and legal regulations associated with transgenic crops. It maximizes genetic gains in breeding programs, is one of the fastest tools available for developing large numbers of inbred lines quickly and reduces the cost of breeding programs. 

“Deployment of doubled haploid technology is much needed for commercial hybrid maize breeding programs to make them more efficient and economical,” says article co-author Abdurahman Beshir, a maize seed systems specialist based in Nepal. “The technology is also useful to have accelerated varietal turnover and a higher maize seed replacement rate in different market segments.”  

Many multinational seed companies have adopted doubled haploid technology for the wide-scale production of inbred lines. The development of novel techniques for haploid induction and the subsequent production of doubled haploid plants holds significant potential for the management of genetic resources, germplasm enhancement and the development of novel plant populations. Researchers at CIMMYT have also made significant efforts to help national breeding programs adopt this technology, especially in South Asia, where the organisation has shared haploid inducers with numerous partners in Pakistan 

But, while this technology can accelerate maize breeding, it still faces challenges at each step of doubled haploid line development and the authors argue there is a need to extensively explore the genetic potential of this technology to continue increasing the genetic gains associated with different breeding programs.  

Read the full article: Doubled haploids in maize: Development, deployment, and challenges

Cover image: A mixture of doubled haploid maize kernels seen in close-up at CIMMYT’s Agua Fria experimental station in Mexico. (PhotoAlfonso Cortés/CIMMYT)

Read more new publications from CIMMYT researchers: 

  1. Choudhary, M., Meena, V. S., Panday, S. C., Mondal, T., Yadav, R. P., Mishra, P. K., Bisht, J. K., & Pattanayak, A. (2021). Long-term effects of organic manure and inorganic fertilization on biological soil quality indicators of soybean-wheat rotation in the Indian mid-HimalayaAppl. Soil Ecol.157. 
  2. Costa-Neto, G., Fritsche-Neto, R., & Crossa, J. (2021). Nonlinear kernels, dominance, and envirotyping data increase the accuracy of genome-based prediction in multi-environment trialsHeredity126(1), 92-106.  
  3. Jat, H. S., Datta, A., Choudhary, M., Sharma, P. C., & Jat, M. L. (2021). Conservation Agriculture: Factors and drivers of adoption and scalable innovative practices in Indo-Gangetic plains of India – a reviewInternational Journal of Agricultural Sustainability19(1), 40-55.  
  4. Jena, P. R., De Groote, H., Nayak, B. P., & Hittmeyer, A. (2021). Evolution of Fertiliser Use and its Impact on Maize Productivity in Kenya: Evidence from Multiple SurveysFood Sec.13(1), 95-111.  
  5. Krishna, V. V., & Kubitza, C. (2021). Impact of oil palm expansion on the provision of private and community goods in rural IndonesiaEcol. Econ.179, 106829.  
  6. Novotny, I. P., Fuentes-Ponce, M. H., Tittonell, P., Lopez-Ridaura, S., & Rossing, W. A. H. (2021). Back to the people: The role of community-based responses in shaping landscape trajectories in Oaxaca, MexicoLand Use Policy100, 104912.  
  7. Romero-Salas, E. A., Navarro-Noya, Y. E., Luna-Guido, M., Verhulst, N., Crossa, J., Govaerts, B., & Dendooven, L. (2021). Changes in the bacterial community structure in soil under conventional and conservation practices throughout a complete maize (Zea mays L.) crop cycleAppl. Soil Ecol.157, 103733.  
  8. Simtowe, F., & De Groote, H. (2021). Seasonal participation in maize markets in Zambia: Do agricultural input subsidies and gender matter? Food Sec.13(1), 141-155.  
  9. Simtowe, F., Makumbi, D., Worku, M., Mawia, H., & Rahut, D. B. (2021). Scalability of Adaptation strategies to drought stress: The case of drought tolerant maize varieties in KenyaInternational Journal of Agricultural Sustainability19(1), 91-105.  
  10. Sserumaga, J. P., Makumbi, D., Oikeh, S. O., Otim, M., Machida, L., Anani, B. Y., Nhamucho, E., Beyene, Y., & Mugo, S. (2021). Evaluation of early-generation tropical maize testcrosses for grain-yield potential and weevil (Sitophilus zeamais Motschulsky) resistanceCrop Protection139, 105384.  

Seed production innovations, conservation agriculture and partnerships are key for Africa’s food security

Members of the International Maize Improvement Consortium Africa (IMIC – Africa) and other maize and wheat research partners discovered the latest innovations in seed and agronomy at Embu and Naivasha research stations in Kenya on August 27 and 28, 2019. The International Maize and Wheat Improvement Center (CIMMYT) and the Kenya Agriculture & Livestock Research Organization (KALRO) held their annual partner field days to present sustainable solutions for farmers to cope with poor soils, a changing climate and emerging diseases and pests, such as wheat rust, maize lethal necrosis or fall armyworm.

Versatile seeds and conservation agriculture offer farmers yield stability

“Maize is food in Kenya. Wheat is also gaining importance for our countries in eastern Africa,” KALRO Embu Center Director, Patrick Gicheru, remarked. “We have been collaborating for many years with CIMMYT on maize and wheat research to develop and disseminate improved technologies that help our farmers cope against many challenges,” he said.

Farmers in Embu, like in most parts of Kenya, faced a month delay in the onset of rains last planting season. Such climate variability presents a challenge for farmers in choosing the right maize varieties. During the field days, CIMMYT and KALRO maize breeders presented high-yielding maize germplasm adapted to diverse agro-ecological conditions, ranging from early to late maturity and from lowlands to highlands.

João Saraiva, from the Angolan seed company Jardins d’Ayoba, said having access to the most recent improved maize germplasm is helpful for his young seed company to develop quality seeds adapted to farmers’ needs. He is looking for solutions against fall armyworm, as the invasive species is thriving in the Angolan tropical environment. He was interested to hear about CIMMYT’s progress to identify promising maize lines resistant to the caterpillar. Since fall armyworm was first observed in Africa in 2016, CIMMYT has screened almost 1,200 inbred lines and 2,900 hybrids for tolerance to fall armyworm.

“Hopefully, we will be developing and releasing the first fall armyworm-tolerant hybrids by the first quarter of 2020,” announced B.M. Prasanna, director of CIMMYT’s Global Maize Programme and the CGIAR Research Program on Maize (MAIZE).

“Through continuous innovations to build varieties that perform well despite dry spells, heat waves or disease outbreak, maize scientists have been able to deliver significant yield increases each year across various environments,” explained Prasanna. “This genetic gain race is important to respond to growing grain demands despite growing climate risks and declining soil health.”

Berhanu Tadesse, maize breeder at the Ethiopian Institute for Agricultural Research (EIAR), was highly impressed by the disease-free, impeccable green maize plants at Embu station, remembering the spotted and crippled foliage during a visit more than a decade ago. This was “visual proof of constant progress,” he said.

For best results, smallholder farmers should use good agronomic practices to conserve water and soil health. KALRO agronomist Alfred Micheni demonstrated different tillage techniques during the field tour including the furrow ridge, which is adapted to semi-arid environments because it retains soil moisture.

Late maturity hybrid demonstration plot at Embu station. (Photo: Jérôme Bossuet/CIMMYT)

Innovations for a dynamic African seed sector

A vibrant local seed industry is needed for farmers to access improved varieties. Seed growers must be able to produce pure, high-quality seeds at competitive costs so they can flourish in business and reach many smallholder farmers.

Double haploid technology enables breeders to cut selection cycles from six to two, ultimately reducing costs by one third while ensuring a higher level of purity. Sixty percent of CIMMYT maize lines are now developed using double haploid technology, an approach also available to partners such as the Kenyan seed company Western Seeds.

The Seed Production Technology for Africa (SPTA) project, a collaboration between CIMMYT, KALRO, Corteva Agriscience and the Agricultural Research Council, is another innovation for seed companies enabling cheaper and higher quality maize hybrid production. Maize plants have both female and male pollen-producing flowers called tassels. To produce maize hybrids, breeders cross two distinct female and male parents. Seed growers usually break the tassels of female lines manually to avoid self-pollination. SPTA tested a male sterility gene in Kenya and South Africa, so that female parents did not produce pollen, avoiding a detasseling operation that damages the plant. It also saves labor and boosts seed yields. Initial trial data showed a 5 to 15% yield increase, improving the seed purity as well.

World-class research facilities to fight new and rapidly evolving diseases

The KALRO Naivasha research station has hosted the maize lethal necrosis (MLN) quarantine and screening facility since 2013. Implementing rigorous phytosanitary protocols in this confined site enables researchers to study the viral disease first observed in Africa 2011 in Bomet country, Kenya. Working with national research and plant health organizations across the region and the private sector, MLN has since been contained.

A bird’s eye view of the demonstration plots is the best testimony of the impact of MLN research. Green patches of MLN-resistant maize alternate with yellow, shrivelled plots. Commercial varieties are susceptible to the disease that can totally wipe out the crop, while new MLN-resistant hybrids yield five to six tons per hectare. Since the MLN outbreak in 2011, CIMMYT has released 19 MLN-tolerant hybrids with drought-tolerance and high-yielding traits as well.

Maize Lethal Necrosis (MLN) sensitive and resistant hybrid demo plots in Naivasha’s quarantine & screening facility (Photo: KIPENZ/CIMMYT)

A major challenge to achieving food security is to accelerate the varietal replacement on the market. CIMMYT scientists and partners have identified the lengthy and costly seed certification process as a major hurdle, especially in Kenya. The Principal Secretary of the State Department for Research in the Ministry of Agriculture, Livestock, and Fisheries, Hamadi Boga, pledged to take up this issue with the Kenya Plant and Health Inspectorate Service (KEPHIS).

“Such rapid impact is remarkable, but we cannot rest. We need more seed companies to pick up these new improved seeds, so that our research reaches the maximum number of smallholders,’’ concluded Prasanna.

CIMMYT and UAS-Bangalore to establish a maize doubled haploid facility in Karnataka, India

Representatives from CIMMYT and UAS-Bangalore signed the collaboration agreement on February 18, 2019.
Representatives from CIMMYT and UAS-Bangalore signed the collaboration agreement on February 18, 2019.

KARNAKATA, India (CIMMYT) — The International Maize and Wheat Improvement Center (CIMMYT) and the University of Agricultural Sciences-Bangalore (UAS-Bangalore) have signed a collaboration agreement for establishing a maize doubled haploid (DH) facility at the Agricultural Research Station in Kunigal (ARS-Kunigal), Tumkur district, Karnataka state, India.

CIMMYT will establish and operate the maize DH facility, including field activities and the associated laboratory. Occupying 12 acres of land, the facility is estimated to produce at least 30,000 DH lines a year. CIMMYT hopes the facility to be operational by the last quarter of 2019.

The maize DH facility, funded by the CGIAR Research Program on Maize (MAIZE), fulfills a very important requirement of the region. It has the potential to accelerate maize breeding and hybrid development and significantly increase genetic gains through maize breeding in Asia. During the 13th Asian Maize Conference in Ludhiana, India (October 8-10, 2018), several partners — including the Indian Institute of Maize Research (ICAR-IIMR) — emphasized the urgent need for a state-of-the-art maize DH facility that could serve breeding programs across Asia.

“This is indeed a major landmark for maize breeding, especially in the public sector, not only in India, but also in Asia,” said B.M. Prasanna, Director of CIMMYT’s Global Maize Program and the CGIAR Research Program on Maize (MAIZE). “The facility will provide maize DH development services, not only for the maize breeding programs of CIMMYT and UAS-B, but also for national agricultural research system institutions and small and medium-sized seed companies engaged in maize breeding and interested to pursue DH-based advanced maize breeding strategies in Asia. DH technology, in combination with molecular marker-assisted breeding, can significantly increase genetic gains in maize breeding.”

“The maize doubled haploid facility … will be the first of its kind in the public domain in Asia,” said S. Rajendra Prasad, Vice Chancellor of UAS-Bangalore. “The work done at this facility will certainly benefit the farmers of the state, country and the Asian region, by accelerating maize breeding and improving efficiencies.”

The signing of the collaboration agreement took place on February 18, 2019 at UAS-Bangalore’s campus in Bengaluru. CIMMYT was represented by B.M. Prasanna and BS Vivek, Senior Maize Breeder. UAS-Bangalore was represented by S. Rajendra Prasad; Mahabaleshwar Hegde, Registrar, and Y.G. Shadakshari, Director of Research.

The benefits of doubled haploid technology

DH maize lines are highly uniform, genetically pure and stable, and enable significant saving of time and resources in deriving parental lines, which are building blocks of improved maize hybrids.

Over the last 12 years, CIMMYT has worked intensively on optimizing DH technology for the tropics. Researchers released first-generation tropicalized haploid inducers in 2012, and second-generation tropicalized haploid inducers in 2017, in partnership with the University of Hohenheim, Germany. In 2017, CIMMYT developed more than 93,000 maize DH lines from 455 populations, and delivered them to maize breeders in Africa, Asia and Latin America.


INTERVIEW OPPORTUNITIES:

B.M. Prasanna – Director of CIMMYT’s Global Maize Program and the CGIAR Research Program on Maize (MAIZE).

FOR MORE INFORMATION, CONTACT THE MEDIA TEAM:

Jennifer Johnson – Maize Communication Officer, CIMMYT. J.A.JOHNSON@cgiar.org, +52 (55) 5804 2004 ext. 1036.

International experts discuss progress and challenges of maize research and development in Asia

The importance of maize in Asian cropping systems has grown rapidly in recent years, with several countries registering impressive growth rates in maize production and productivity. However, increasing and competing demands — food, feed, and industry — highlight the continued need to invest in maize research for development in the region. Maize experts from around the world gathered to discuss these challenges and how to solve them at the 13th Asian Maize Conference and Expert Consultation on Maize for Food, Feed, Nutrition and Environmental Security, held from October 8 to 10, 2018, in Ludhiana, Punjab, India.

More than 280 delegates from 20 countries attended the conference. Technical sessions and panel discussions covered diverse topics such as novel tools and strategies for increasing genetic gains, stress-resilient maize, sustainable intensification of maize-based cropping systems, specialty maize, processing and value addition, and nutritionally enriched maize for Asia.

The international conference was jointly organized by the Indian Council of Agricultural Research (ICAR), the International Maize and Wheat Improvement Center (CIMMYT), the Indian Institute of Maize Research (ICAR-IIMR), Punjab Agricultural University (PAU), the CGIAR Research Program on Maize (MAIZE), and the Borlaug Institute for South Asia (BISA).

In Asia, maize is rapidly growing in its importance, due to high demand. Maize productivity in the region is growing by 5.2 percent annually compared to a global average of 3.5 percent. However, this is not enough. “Asia produces nearly 80 million tons of maize annually, but demand will be double by the year 2050,” said Martin Kropff, CIMMYT director general, in his opening address at the conference. “We need to produce two times more maize in Asia, using two times less inputs, including water and nutrients. Climatic extremes and variability, especially in South and South East Asia, will make this challenge more difficult. Continued funding for maize research is crucial. We need to work together to ensure that appropriate innovations reach the smallholder farmers.”

Field visit in Ludhiana, India, during the 13th Asian Maize Conference. (Photo: Manjit Singh/Punjab Agricultural University)
Field visit in Ludhiana, India, during the 13th Asian Maize Conference. (Photo: Manjit Singh/Punjab Agricultural University)

Climate-resilient maize and sustainable intensification

A major theme emphasized at the conference was climate resilience in maize-based systems. South Asia is a hotspot for vulnerability due to climate change and climate variability, which poses great risks to smallholder farmers. “Climate resilience cannot be brought by only a single technology — it has to be through a judicious mix of several approaches,” said B.M. Prasanna, director of CIMMYT’s Global Maize Program and the CGIAR Research Program on Maize.

Great advances have been made in developing climate-resilient maize for Asia since the last Asian Maize Conference, held in 2014. Many new heat- and drought-tolerant maize varieties have been developed through various projects, such as the Heat Stress Tolerant Maize for Asia (HTMA), and Affordable, Accessible, Asian (AAA) maize projects. Through the HTMA project, over 50 CIMMYT-derived elite heat-tolerant maize hybrids have been licensed to public and private sector partners in Asia during the last three years, and nine heat-tolerant maize hybrids have been released so far in Bangladesh, India and Nepal.

Sustainable intensification of maize-based farming systems has also helped farmers to increase yields while reducing environmental impact, through conservation agriculture and scale-appropriate mechanization. Simple technologies are now available to reduce harvest time by up to 80 percent and hired labor costs by up to 60 percent. Researchers across the region are also working to strengthen the maize value chains.

B.S. Dhillon (center) receives the MAIZE Champion Award for his pioneering work in maize breeding. (Photo: Manjit Singh/Punjab Agricultural University)
B.S. Dhillon (center) receives the MAIZE Champion Award for his pioneering work in maize breeding. (Photo: Manjit Singh/Punjab Agricultural University)

Science and appropriate technologies

CIMMYT has been focusing on developing and deploying new technologies that can enhance the efficiency of maize breeding programs; these include doubled haploid (DH) technology, high-throughput field-based phenotyping, and genomics-assisted breeding. The conference emphasized on the need for Asian institutions to adapt such new tools and technologies in maize breeding programs.

Another topic of interest was the fall armyworm, an invasive insect pest that has spread through 44 countries in Africa and was recently reported in India for the first time. “This pest can migrate very quickly and doesn’t require visas and passports like we do. It will travel, and Asian nations need to be prepared,” Prasanna said. “However, there is no need for alarm. We will be looking at lessons learned from other regions and will work together to control this pest.”

In addition to grain for food and feed, specialty maize varieties can provide beneficial economic alternatives for smallholder maize farmers. Conference participants had the opportunity to hear from Indian farmers Kanwal Singh Chauhan and Yugandar Y, who have effectively adopted specialty maize varieties, such as baby corn, sweet corn and popcorn, into life-changing economic opportunities for farming communities. They hope to inspire other farmers in the region to do the same.

On October 10, conference delegates participated in a maize field day organized at the BISA farm in Ladhowal, Ludhiana. Nearly 100 improved maize varieties developed by CIMMYT, ICAR and public and private sector partners were on display, in addition to scale-appropriate mechanization options, decision support tools, and precision nutrient and water management techniques.

The conference concluded with a ceremony honoring the winners of the 2018 MAIZE-Asia Youth Innovators Award. The awards were launched in collaboration between the CGIAR Research Program on Maize and YPARD (Young Professionals for Agricultural Development) to recognize the contributions of innovative young women and men who can inspire fellow youth to get involved in improving maize-based agri-food systems in Asia. Winners of the first edition of the awards include Dinesh Panday of Nepal, Jie Xu of China, Samjhana Khanal of Nepal, and Vignesh Muthusamy of India.

Participants listen to a briefing during the field visit of the 13th Asian Maize Conference, in Ludhiana, India. (Photo: Manjit Singh/Punjab Agricultural University)
Participants listen to a briefing during the field visit of the 13th Asian Maize Conference, in Ludhiana, India. (Photo: Manjit Singh/Punjab Agricultural University)

CIMMYT shows partners in Kenya new breakthroughs in maize and wheat research

NAIROBI (Kenya) — Members of the International Maize Improvement Consortium (IMIC) and other partners had a chance to go on a field visit to the Kiboko and Naivasha research stations in Kenya on September 18 and 19, 2018. The International Maize and Wheat Improvement Center (CIMMYT) and the Kenya Agriculture & Livestock Research Organization (KALRO) held their annual partner field days to share the latest developments in maize and wheat research.

On the first day, CIMMYT invited IMIC researchers to evaluate Material Under Development at the Kiboko site. These maize lines are not publicly released yet but are available to IMIC partners, so they can select the most promising ones for their research and crop improvement work.

Each seed company was looking for certain traits to develop new hybrid varieties. For instance, Samit Fayek, from Fine Seeds Egypt was looking for ‘erect type’ maize, as he wants higher crop density and grains that look big. Christopher Volbrecht, from Lake Agriculture in South Africa, was looking for “cobs that stick out as this is what farmers want.” Josephine Okot, from Victoria Seeds in Uganda, said that “seed companies often look at drought tolerance only, but we need now to integrate resistance to Maize Lethal Necrosis.”

Using Doubled Haploid breeding in Kiboko

Some of the workers at Kiboko station sorting out maize seed varieties. (Photo: Joshua Masinde/CIMMYT)
Some of the workers at Kiboko station sorting out maize seed varieties. (Photo: Joshua Masinde/CIMMYT)

Next on the tour to Kiboko, partners visited various stress-tolerant breeding materials, sustainable intensification cropping demonstrations and the Doubled Haploid facility. Vijaya Chaikam, Maize Doubled Haploid Scientist, explained how CIMMYT uses this methodology to cut down breeding time from six to two cycles, which drastically reduces costs.

According to B.M. Prasanna, director of CIMMYT’s Global Maize Program and the CGIAR Research Program MAIZE, doubled haploid breeding is possibly the biggest innovation to speed up genetic gain since the inception of hybrid technology a century ago. “In the next 4 or 5 years, CIMMYT aims at 80 percent use of double haploid lines for new hybrid development; breeding will be faster and much cheaper that way,” Prasanna said. “For now, breeders and seed companies need to know how to use double haploid lines to cost-efficiently crossbreed with their varieties for high-quality hybrids.”

At the end of the visit to Kiboko, CIMMYT officially opened a new maize seed storage cold room. This facility will serve to keep seeds in good condition and to better manage inventory. At the opening were the director of KALRO’s Food Crops Research Institute, Joyce Malinga, CIMMYT’s Africa Regional Representative, Stephen Mugo, and CIMMYT’s Technical Lead for the Global Maize Program, Aparna Das.

Fighting Maize Lethal Necrosis and rust in Naivasha

A worker at the Naivasha MLN research station conducts a mock inoculation (Photo: Joshua Masinde/CIMMYT)
A worker at the Naivasha MLN research station conducts a mock inoculation (Photo: Joshua Masinde/CIMMYT)

On the second day, partners visited the Naivasha research station. There, CIMMYT presented the latest efforts to contain Maize Lethal Necrosis (MLN), a devastating maize viral disease first reported in Kenya in 2011 which caused severe crop losses across Eastern Africa, causing severe crop losses. The Naivasha research station is home to a world-class facility to screen for Maize Lethal Necrosis, jointly managed by CIMMYT and KALRO.

At the facility, maize lines are evaluated for MLN resistance. The best lines and varieties are nominated for further development and shared with partners. National Agriculture Research partners can request MLN screening at no cost, while private seed companies are charged for the service. In the last four years, more than 150,000 germplasm have been screened.

CIMMYT wheat scientist Mandeep Randhawa explained how to recognize the different types of wheat rust diseases: stem, stripe and leaf rusts. He emphasized the Ug99 black stem rust strain, which appeared in Uganda in 1998 and has since severely impacted wheat production in the region and globally. Randhawa explained how CIMMYT develops varieties resistant to stem rust using a phenotyping platform and marker-assisted selection.

These two field days were a great opportunity to showcase progress in developing more resilient maize varieties in a fast and cost-effective way. This responsiveness is crucial as pests and diseases continue to threaten the livelihoods of African smallholders. Such impact could not happen without the strong collaboration between CIMMYT and KALRO.

The director of KALRO's Food Crops Research Institute, Joyce Malinga (left), the director of CIMMYT Global Maize Program, B.M. Prasanna (center), and CIMMYT's Regional Representative, Stephen Mugo, open the maize seed cold room in Kiboko (Photo: Joshua Masinde/CIMMYT)
The director of KALRO’s Food Crops Research Institute, Joyce Malinga (left), the director of CIMMYT Global Maize Program, B.M. Prasanna (center), and CIMMYT’s Regional Representative, Stephen Mugo, open the maize seed cold room in Kiboko (Photo: Joshua Masinde/CIMMYT)

The Doubled Haploid Facility in Kiboko and the Maize Lethal Necrosis screening facilty in Naivasha were opened in 2013 with support from the Bill & Melinda Gates Foundation and the Syngenta Foundation.

The International Maize Improvement Consortium (IMIC) is a public-private partnership initiative launched in May 2018 as part of CIMMYT’s mission to ramp up seed breeding and production innovations.