Doubled haploids speed development of drought tolerant maize for Africa
CIMMYT E-News, vol 5 no. 5, May 2008
CIMMYT is adapting an advanced technologyâthe doubled haploid approachâto develop inbred lines of tropical maize for sub-Saharan Africa. It promises to reduce costs and speed the arrival of better-adapted maize for resource-poor farmers in the worldâs toughest environments.
CIMMYT scientists have begun developing drought tolerant varieties of tropical maize for places like sub-Saharan Africa using a high-tech approachâknown as doubled haploidsâpreviously applied principally by commercial seed companies working mostly on temperate maize.
âHaploidâ refers to the number of chromosomes in a reproductive cell, like sperm or ovum. In grasses like maize, the reproductive cellsâpollen and ovulesâcontain half the chromosomes of a full-grown individual. Fertilization joins the genetic information from the two parents, and offspring carry paired sets of chromosomes, reflecting the diversity of each parent.
âMaize breeders working on hybridsâthe most productive type of maize variety and the one marketed by most seed companiesâmust at some point create genetically-stable and pure lines of desirable, individual plants, for use as parents of hybrids,â says CIMMYT maize physiologist Jose Luis Araus. Conventionally, breeders get the lines by repeatedly fertilizing selected, individual maize plants with the plantâs own pollen. The process requires expensive field space, labor, and timeânormally, seven or more generations, which represents at least three years, even in settings where itâs possible to grow two crops per season.
Purer, faster, cheaper
In the latter part of the 20th century, crop scientists developed a quicker, cheaper path to genetically-uniform parent linesâthough a technically intricate method. The first step involves crossing normal maize with special maize types called âinducers,â whose pollen causes the normal maize to produce seed containing haploid embryos. The haploid embryo carries a single set of its own chromosomes, rather than the normal paired sets. The embryos are planted, and subsequent treatment of the seedlings with a particular chemical causes them to make âphotocopiesâ of their haploid chromosomes, resulting in a fertile plant endowed with a doubled set of identical chromosomes and able to produce seed of 100% genetic purity. âThe actual treatment, as well as getting from the embryo to a reasonable amount of seed of the pure line, is very complicated,â says Ciro SĂĄnchez RodrĂguez, CIMMYT technician in charge of doubled haploid field trials, âbut when the process is perfected, it only takes two generationsâabout one yearâand the logistical advantages are tremendous.â

First extensive use in the tropics
CIMMYT is implementing the doubled haploid technology on a research station in Mexico, using drought tolerant plants adapted to sub-Saharan Africa. âCIMMYTâs use of the practice is another example of how we put advanced technologies at the service of disadvantaged, small-scale farmers,â says Araus. âAmong other things, this represents a significant opportunity to increase the availability of improved, drought tolerant maize varieties for sub-Saharan Africa,â he says.
Commercial seed companies in Europe and North America have been the main users of the doubled haploid technology, and the inducer genotypes available are of temperate adaptation. âThe inducers perform very poorly in the tropical conditions of our Mexico stations,â says Vanessa Prigge, a PhD student from the University of Hohenheim working at CIMMYT to perfect the technique. To generate inducers that work better in tropical settings, Prigge and colleagues are crossing temperate inducers from Hohenheim with CIMMYT maize from Mexico, Kenya, and Zimbabwe. âWe expect to have tropical versions of the inducers in a couple years,â she says.
Reaching farmersâ fields
Maize lines from this work will be used initially in the Drought Tolerant Maize for Africa (DTMA) and the Water Efficient Maize for Africa (WEMA) projects.
âThis is a very exciting technology,â says Aida Kebede, an Ethiopian PhD student from Hohenheim helping to establish the doubled haploid technology at CIMMYT. âIt holds the key to addressing more quickly the persistent problems of African maize growers: drought, disease pressure, and low productivity. Iâm happy to contribute!â


Speaking at the SIMLESAâs second âbirthday party,â Joana Hewitt, chairperson of the ACIAR Commission for International Agricultural Research, reiterated the Australian governmentâs commitment to long-term partnerships with African governments. Participants also heard of the new SIMLESA Program in Zimbabwe, focusing on crop-livestock interactions. During the dinner, Kenya and Mozambique were recognized for their efforts in promoting and strengthening local innovation platforms.
Tunga Silvar grows maize to feed his wife and fourgrandchildren on about 0.5 hectares of land in Mawanga, Zimbabwe, a hilly area some 45 kilometers northeast of Harare. Like otherfarmers in the region, he is acutely aware of the value of nitrogen fertilizer, continually juggles his limited household financesto get it, and is poorer and hungrier when he canât. âWe used to sell maize, but in the last five years we havenât been able to do so,â saysSilvar. âI had to pay school fees for my grandchildren, so I couldnât buy fertilizer. Fertilizer is very important, especially in our type of soil. If you donât apply it, youcan barely harvest anything.”
Late and erratic rainfall in Zimbabwe has many farmers facing the prospect of poor harvests. The current hardships from drought though may furnish some hopefor farmers. New drought tolerant varieties are being tested in on-farm trials under farmer management. Many of the trials are experiencing drought stressâaperfect opportunity to identify the best varieties for such harsh conditions. A recent visit to on-farm trials in the Murewa District of Zimbabwe showed many new drought tolerant products performing well. Local farmer Sailas Ruswa is growing a trial and was enthusiastic about what he saw: some varieties showedsigns of severe drought stress, but a few were holding up well and were expected to produce good yields.
Paul Mapfumo, member of the global conservation agriculture program and coordinator of SOFECSAâthe Soil Fertility Consortium for Southern Africaâhas been awarded a full professorship at the University of Zimbabwe in Harare as of March 2012, with the details of his continued relationship with CIMMYT to be worked out. Having opened its doors in 1952, the University is the oldest and largest university in Zimbabwe and has 10 faculties, including agriculture, as well as specialist research centers. âThe SOFECSA work in CIMMYTâso much appreciated by communities and partners in Zimbabweâwas a major driver for this achievement,â said Mapfumo. âSo thanks to CIMMYT and all our great partners.â SOFECSA is an inter-institutional and interdisciplinary consortium to undertake field- level adaptive research and development activities that enhance the impacts of integrated soil fertility management technologies on food security and farm incomes in Southern Africa and beyond.
A training course was held at the Agricultural Research Trust (ART), in Harare, Zimbabwe during 12-15 March 2012. Organized by CIMMYT-Zimbabwe, the course aimed to improve the skills and knowledge of maize technicians regarding implementing on-station and on-farm trials, seed production, and the use of secondary traits in selecting superior genotypes under low N and drought trials.
On 14 November 2011 Thokozile Ndlela and Charles Mutimaamba of Zimbabweâs Crop Breeding Institute received the DTMA Breeding Team Award for southern Africa. It was presented by Cosmos Magorokosho, CIMMYT Zimbabwe Maize Breeder, at a management meeting of the Department of Research and Specialist Services of the Ministry of Agriculture, Mechanisation and Irrigation Development of the Government of Zimbabwe, at their head office in Harare. The award consisted of USD 3000 and a silver tray that the winners will be able to display until it is reawarded next year.
Is it possible for a software to act as a one-stop shop and help manage maize breeding? Indeed it is, and CIMMYTâs Fieldbook provides both.
It was a short visit but by no means an unproductive one: from 18â22 May 2009, a Corporate Services team from CIMMYT-El BatĂĄn visited the centerâs Nairobi and Harare regional offices. The team consisted of Luis De Anda, finance manager; Marisa de la O, human resources (HR) manager; Carlos LĂłpez, information and communication technology (ICT) manager; and Scott Ferguson, deputy director general for Corporate Services.
In Zimbabwe, the team visited the new dam that supplies water to CIMMYTâs Muzarabani station âthe old dam was destroyed by flooding. Joseph Makamba, field supervisor, also showed them the stationâs winter nursery, which had over seven hectares dedicated to nurseries and seed multiplication. Unfortunately, frequent power outages make nursery irrigation sporadic; during the visit Makamba had to travel 60 km to bring national power supply technicians to repair overhead power lines and restore electricity to the pumping station.
De la O was especially touched by the temporary workers she met in Harare, many of whom are mothers that leave their families for months at a time to make ends meet. âI would like for all employees to have the same level of benefits, regardless of where theyâre from or if they are temporary or permanent,â she said. âWe want to offer a better level of support and work in a global sense.â De la O addressed some issues on the spot, and will followup with others after an analysis of all the regional offices and making a strategic HR plan.