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MLN diagnostics and management in Africa through multi-institutional synergies

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MLN coverMaize Lethal Necrosis (MLN) disease has continued to wreak havoc on maize production in East Africa since it was first reported in Kenya in 2011, and since then reported in the Democratic Republic of the Congo, Ethiopia, Rwanda, Tanzania, South Sudan and Uganda. The disease, caused by a combination of the Maize Chlorotic Mottle Virus (MCMV) and Sugarcane Mosaic Virus (SCMV), causes irreversible damage that kills maize plants before they can grow and yield grain. MLN pathogens can be transmitted not only by insect vectors but also through contaminated seed. The epidemic is exacerbated by lack of MLN-resistant maize varieties and year round cultivation of maize in many areas in eastern Africa, enabling the build-up of virus inoculum and allowing transmission via insect vectors. For this reason, CIMMYT scientists Monica Mezzalama, Biswanath Das, and B.M. Prasanna have developed a brochure “MLN Pathogen Diagnosis, MLN-free Seed Production and Safe Exchange to Non-Endemic Countries” for providing important information on these key areas to stakeholders, especially seed companies and regulatory agencies operating in both MLN-affected as well as MLN non-endemic countries.

“MLN is an increasing regional threat to food security in sub-Saharan Africa, and must be tackled with concerted effort from all actors in order to safeguard the maize seed sector and protect the livelihoods of smallholder farmers,” said Prasanna. The brochure proposes several key steps to curb the spread of MLN, through MLN diagnostics, production of MLN-free seed, and safe exchange to MLN-endemic countries. The brochure also advises on appropriate agronomic practices that can prevent disease incidence in seed production fields.

An International Conference on “MLN Diagnostics and Management in Africa” will be organized jointly by AGRA (Alliance for Green Revolution in Africa) and CIMMYT during 12-14 May in Nairobi, Kenya, in order to review the present status of MLN incidence and impacts in sub-Saharan Africa (SSA), controlling seed transmission of MLN, managing seed production in MLN-endemic countries, creating awareness about MLN diagnostic protocols, and identifying ways to strengthen MLN diagnostics capacity in SSA, among other topics.

Maize lethal necrosis: a serious threat to food security in eastern Africa and beyond

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MLN_WS_participants_w
Participants are shown how to inspect maize fields for MLN symptoms and how to collect samples for laboratory analysis.

Maize lethal necrosis (MLN) has rapidly emerged as one of the deadliest maize diseases in eastern Africa capable of causing complete yield loss under heavy disease pressure. This means that Kenya and neighboring countries which largely depend on maize as their main staple food and source of income are on the verge of a looming food and economic crisis.

The disease is difficult to control for two reasons: firstly, it is caused by a combination of viruses; secondly, it can be spread through seed and by insect vectors that may be carried by wind over long distances. Affected crops suffer various symptoms such as severe stunting, tassel abnormality, small ears with poor seed set, chlorotic leaf mottling, leaf necrosis and premature plant death.

Much more than CIMMYT and East Africa

Sixty phytosanitary regulators and seed industry scientists from 11 countries in eastern and southern Africa attended an MLN diagnostics and screening workshop from March 17–19, 2015, in Naivasha, Kenya. The objective of the workshop was to train scientists on the latest MLN diagnostics and screening methods and to share knowledge on how to control the spread of MLN. Besides DR Congo, Ethiopia, Kenya, Rwanda and Tanzania where the disease has been reported, other participants were from South Sudan and southern Africa (Malawi, Mozambique, South Africa, Zambia and Zimbabwe) that have no confirmed cases of MLN, but where maize is an important crop.

CIMMYT organized the workshop in response to the high demand for development of appropriate diagnostics methods and harmonization of regional protocols. Hence, facilitation by agencies like the Food and Agricultural Organization provided a much-needed regional overview of the MLN threat, in addition to perspectives from the International Centre of Insect Physiology Ecology and the Kenya Plant Health Inspectorate Services (KEPHIS) on MLN insect vectors and diagnostics methods respectively.

The workshop was conducted at the MLN screening facility in Naivasha, the largest of its kind established in response to the MLN outbreak in eastern Africa in 2013. It supports countries in the sub-Saharan region to screen seeds under artificial inoculation. The facility is managed jointly by the Kenya Agricultural and Livestock Research Organization (KALRO) and CIMMYT, and was established with support from the Bill & Melinda Gates Foundation and the Sygenta Foundation for Sustainable Agriculture. Biswanath Das, a maize breeder at CIMMYT, noted that “the site has evaluated more than 20,000 accessions since its inception in 2013 from over 15 multinational and national seed companies and national research programs.” This, he added, “has become a primary resource in the fight against MLN regionally.”

Collective pre-emptive actions for prevention: seeds of hope
Participants received hands-on training to identify symptoms of MLN-causing viruses and how to score disease severity by screening germplasm at the site. For some participants, this was a first. “This is my first time to see an MLN-infected plant. Now I understand the impact of MLN on maize production and the need to set up a seed regulatory facility. South Sudan has no laboratory to test planting materials. My first step will be to talk to my counterparts in the ministry to set up one,” said Taban James, a regulator from the Ministry of Agriculture in South Sudan.

DAS-ELISA_demo_w.jpg
CIMMYT staff demonstrate DAS–ELISA method used for detecting MLN-causing viruses.

The tragic reality is that almost all commercial maize varieties in East Africa are highly susceptible to MLN, based on evaluations done at the screening facility. Therefore, stronger diagnostic and sampling capacity at common border-points was agreed to be a key step towards controlling inadvertent introduction of MLN through contaminated seeds. This was particularly important for participants from southern Africa countries who noted an urgent need for surveillance at seed import ports and border areas to contain the spread.

Currently, Kenya, Uganda and Zimbabwe are the only countries that require imported seed to be certified as free of MLN-causing viruses. KEPHIS and CIMMYT have worked closely to restrict movement of germplasm from Kenya to countries in East Africa with reported MLN cases. Seed production fields are inspected thrice by KEPHIS, in addition to analysis of final seed lots. Plans are underway for CIMMYT in collaboration with the ministries of agriculture in Mexico and Zimbabwe to establish quarantine sites to ease germplasm movement in and out of these countries. Speaking on KEPHIS’ role, Francis Mwatuni, the officer-in-charge of Plant Quarantine and Biosecurity Station said, “We ensure all seed fields are inspected and samples tested for MLN resistance including local and imported seed lots from seed companies, to ensure that farmers get MLN-free seeds.”

The latest trends and options for diagnostics on MLN-causing viruses were covered as well, giving participants hands-on training using ELISA diagnostics systems. They were also briefed on polymerase chain reaction based diagnostics and the latest lateral flow diagnostic kits that are under development that will enable researchers to obtain diagnostic results in the field in minutes.

What next for MLN?
The rapid multiplication of the disease coupled with uncertainties over its spread is the biggest hurdle that scientists and other stakeholders are grappling with. KALRO Chief Researcher, Anne Wangai, who played a key role in discovering the disease in Kenya in 2011 observes that “The uncertainties over the transmission of MLN is a worrying phenomenon that requires stakeholders to urgently find a control point to manage and ensure seeds being given to farmers are MLN-free.”

Breeding remains a key component in the search for long-term solution for MLN, and several milestones have been covered to develop MLN-resistant varieties in East Africa. “CIMMYT has developed five hybrids with good MLN tolerance under artificial inoculation, which have either been released or recommended for release in Kenya, Uganda and Tanzania. Thirteen hybrids are currently under national performance trials in the three countries,” noted Mosisa Regasa, a maize seed system specialist at CIMMYT. He further added that it is critical for the MLN-tolerant hybrids to also have other traits important to farmers, so farmers accept these new hybrids.

Open information sharing forums like the diagnostics workshop are an important step to raise awareness and seek solutions to manage the disease. Sharing best practice and lessons learnt in managing the disease are major steps towards curbing MLN. In pursuit of this end, a major international conference on MLN opens next week.

Links: Slides from the workshop | Workshop announcement |Open call for MLN screening – May 2015

Maize lethal necrosis screening facility seeing signs of success

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CIMMYT pathologist George Mahuku and MLN technician Janet Kimunye examine tassels for pollen production on an infected plant. MLN causes a symptom called ‘tassel blast’ where the tassels of infected plants do not shed or produce pollen. Photos courtesy of George Mahuku

By George Mahuku and Florence Sipalla/CIMMYT

Germplasm screening at the maize lethal necrosis (MLN) screening facility at the Kenya Agricultural Research Institute (KARI) Naivasha is underway, and CIMMYT pathologist George Mahuku said some inoculated lines are showing levels of resistance.

He described the green islands among the maze of yellow in the fields as a demonstration of the success of the testing protocols being used at the site. “This is the lifeline for farmers,” he said. “Next we will be incorporating genes from these lines into adapted germplasm and using the Doubled Haploid facility in Kiboko to quickly develop inbred lines with resistance to MLN.”The deadly maize disease was first identified in Kenya in 2011 and has since been diagnosed in the Democratic Republic of Congo, Rwanda, Tanzania and Uganda. The MLN screening facility was established in 2013 with funding from the Bill & Melinda Gates Foundation and the Syngenta Foundation for Sustainable Agriculture to serve maize breeding institutions in Sub-Saharan Africa in response to 2014 the emergence of the disease.

CIMMYT pathologist George Mahuku inspecting plants that show tolerance to MLN in Naivasha, Kenya.

“To date, we have planted more than 19,000 different types of germplasm on 15 hectares,” Mahuku said. “This germplasm was submitted by both private and public sector partners, including CIMMYT and the International Institute of Tropical Agriculture (IITA).”

All germplasm has been inoculated, Mahuku said, and symptoms are fully visible. Operations at the facility include maintaining pure strains of the viruses that cause MLN, producing inoculum for artificial inoculation, evaluating maize hybrids and inbred lines for response to MLN and building the capacity of stakeholders including scientists, technicians, farmers and extension workers to handle the disease.

The facility also provides employment opportunities for the community, hiring more than 30 people for activities such as weeding, irrigation and disease scoring. Because the facility screens germplasm from different countries, it’s isolated from farmers’ maize plots and certified as a quarantine site. “We still do not fully understand the variability in virus strains, whether the virus strains in Rwanda, Tanzania or Uganda are the same as the ones in Kenya,” said Mahuku.

After disease evaluations, all plant debris will be disposed of by incineration. The facility has received many visitors from universities, international organizations and public and private institutions. “There is a lot of interest in learning and knowing the disease,” Mahuku said.

It recently hosted two scientists from Ethiopia who will share the knowledge gained with their colleagues and will conduct surveys to assess whether the disease is in their country. “To see all this going well is breathtaking,” said CIMMYT technician Janet Kimunye, who is in charge of virus maintenance, inoculum production and inoculations in the field and has been involved in MLN research from the beginning, initially as a consultant to CIMMYT.

“We have assembled a really good team here; watching them work way into the night and weekend is heartening,” said Mahuku. “Everybody wants a solution to this problem that is threatening their food security.”

One of the greenhouses where artificial inoculum is produced and multiplied for research purposes.

Facts about the Facility:

  • Area planted: 15 ha
  • Number of rows: 49,500
  • Total germplasm: 19,539
  • Inoculated area: 6.5 ha
  • Disease expression: 4.5 ha is under disease evaluation as symptoms are expressing well

Sources of Germplasm Sources of Entries Screened

  • CIMMYT/IITA: 13,699
  • Private sector (seed companies): 3,781
  • Public institutions (NARS): 2,059
  • Total: 19,539

Update: CIMMYT maize inbred lines and pre-commercial hybrids with potential resistance to maize lethal necrosis (MLN)

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By B.M. Prasanna/CIMMYT

Since 2011 MLN has become a disease of serious concern in Kenya, Tanzania, Uganda and possibly Rwanda. CIMMYT has been working in close collaboration with the Kenya Agricultural Research Institute (KARI), private sector partners and virology experts from the USA to combat the disease through host-controlled resistance.

MLN-susceptible line (CML505) versus MLN-resistant inbred line (CLRCY034).

A CIMMYT-KARI MLN screening facility was established at Naivasha in September 2013, and a large array of maize germplasm is presently being evaluated against the disease under artificial inoculation. Subsequent to the development of effective protocols, CIMMYT and KARI have been conducting MLN screening trials in Kenya since 2012, to identify promising inbred lines and pre-commercial maize hybrids with resistance to MLN.

A promising pre-release CIMMYT hybrid versus an MLN-susceptible commercial check.

This is the second update of the information on potential MLN-resistant or moderately resistant inbred lines and pre-commercial hybrids (crossed with CIMMYT varieties), following the first update that was shared with public and private sector partners in May 2013. An evaluation of CIMMYT inbred lines and pre-commercial hybrids was done under artificial inoculation during 2013-2014 in two independent trials, at Narok and Naivasha sites in the Rift Valley, Kenya (Tables 1 and 2).

In each trial, the entries were grown in at least two replications, and MLN severity scores were recorded at different stages of crop growth (vegetative and reproductive stages) on a 1-5 scale (1 = no disease symptoms, 5 = extensive damage). The highest MLN severity score recorded across different trials on a particular entry, and the corresponding disease response rating, are presented here. For a list of the new lines and hybrids, click here.

 

Update: CIMMYT maize inbred lines and pre-commercial hybrids with potential resistance to maize lethal necrosis (MLN)

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A promising pre-release CIMMYT hybrid versus an MLN-susceptible commercial check. Since 2011 MLN has become a disease of serious concern in the east African countries of Kenya, Tanzania, Uganda and possibly Rwanda. CIMMYT has been working in close collaboration with the Kenya Agricultural Research Institute (KARI), private sector partners and virology experts from the USA to combat the disease through host-controlled resistance. A CIMMYT-KARI MLN Screening Facility was established at Naivasha in September 2013, and a large array of maize germplasm is presently being evaluated against the disease under artificial inoculation. Subsequent to the development of effective protocols, CIMMYT and KARI have been conducting MLN screening trials in Kenya since 2012, to identify promising inbred lines and pre-commercial maize hybrids with resistance to MLN.  This is the second update of the information on potential MLN-resistant or moderately resistant inbred lines and pre-commercial hybrids (in CIMMYT genetic backgrounds), following the first update that was shared with public and private sector partners in May 2013.

An evaluation of CIMMYT inbred lines and pre-commercial hybrids was done under artificial inoculation during 2013-2014 in two independent trials, at Narok and Naivasha sites in the Rift Valley, Kenya (Tables 1 and 2).  In each trial, the entries were grown in at least two replications, and MLN severity scores were recorded at different stages of crop growth (vegetative and reproductive stages) on a 1-5 scale (1 = no disease symptoms, 5 = extensive damage). The highest MLN severity score recorded across different trials on a particular entry, and the corresponding disease response rating, are presented here.

Table 1. Responses of selected CIMMYT maize inbred lines to artificial inoculation with MLN
at Narok and Naivasha, Kenya (2013-2014)

Inbred line

Kernel color

Heterotic Group

Max. MLN severity score

Disease response rating
CLRCY039

Y

B

2.0

R
CLYN261

Y

A

2.0

R
CLRCY034

Y

B

2.0

R
CKDHL120552

W

A

2.3

MR
CKDHL120161

W

B

2.4

MR
CKDHL120668

W

B

2.4

MR
CKDHL120664

W

B

2.4

MR
CML494

W

B

2.5

MR
TZMI730*

W

B

2.5

MR
CKDHL120918

W

B

2.5

MR
CML550

W

B

2.6

MR
CML543 (CKL05003)

W

B

2.7

MR
CKDHL120671

W

B

2.7

MR
CLA106

Y

B

2.7

MR
CKSBL10205

W

AB

2.7

MR
CKSBL10194

W

AB

2.8

MR
CML535 (CLA105)

Y

B

2.8

MR
CKSBL10060

W

A

2.9

MR
CKDHL121310

W

B

3.0

MR
DTPYC9-F46-1-2-1-2-B

Y

A

3.0

MR
CKDHL0500

W

B

3.0

MR

*IITA Inbred Line

Abbreviations
Y: Yellow; W: White
Disease Response Rating
R: Resistant (max. MLN severity score ≀2.0)
MR: Moderately resistant (max. MLN severity
score ≄2.0 but ≀3.0)
S: Susceptible (max. MLN severity score >3.0)		 MLN Severity Scoring (1-5 Scale)
1 = No MLN symptoms
2 = Fine chlorotic streaks on lower leaves
3 = Chlorotic mottling throughout plant
4 = Excessive chlorotic mottling and dead heart
5 = Complete plant necrosis

 

Table 2. Responses of selected CIMMYT pre-commercial hybrids under artificial inoculation
against MLN at Narok and Naivasha, Kenya (2013-2014)

Hybrid Pedigree

Max. MLN severity score

Disease response rating
CKH12613 Under NPT in Tanzania

2.25

MR
CKH12622 CML444/CML445//CLWN234

2.33

MR
CKH12603 Under NPT in Uganda

2.37

MR
CKH12623 CML539/CML442//CLWN234

2.38

MR
CKH12624 CML539/CML442//CML373

2.45

MR
CKIR12014 CML312/CML442// CKSBL10028

2.49

MR
CKH12625 CML444/CML445//CML373

2.50

MR
CKIR12007 CML78/P100C6-200-1-1-B-B-B-B-B// CKSBL10014

2.50

MR
CKDHH0970 CKDHL0089/CKDHL0323//CKDHL0221

2.50

MR
CKIR11024 CML78/P300C5S1B-2-3-2-#-#-1-2-B-B-#// CKSBL10060

2.51

MR
CKH12607 Under NPT in Tanzania

2.51

MR
CKH10085 Under NPT in Kenya

2.62

MR
CKH12600 Under NPT in Uganda and Tanzania

2.66

MR
CKH12627 CLRCW106//CML444/CML395

2.70

MR
CKDHH0943 CKDHL0159/CKDHL0282//CKDHL0214

2.75

MR
CKDHH0945 CKDHL0089/CML395//CKDHL0214

2.75

MR
CKH12626 CML395/CML488//CML373

2.77

MR

Note: The responses of the promising hybrids mentioned above are being validated through
experiments at the MLN Screening Facility in Naivasha

MLN Severity Scoring (1-5 Scale)
1 = No MLN symptoms
2 = Fine chlorotic streaks on lower leaves
3 = Chlorotic mottling throughout plant
4 = Excessive chlorotic mottling and dead heart
5 = Complete plant necrosis		 Disease Response Rating
R: Resistant (max. MLN severity score ≀2.0)
MR: Moderately resistant (max. MLN severity
score ≄2.0 but ≀3.0)

 

 For further information on:

  • MLN research-for-development efforts undertaken by CIMMYT, please contact:
    Dr. B.M. Prasanna, Director, Global Maize Program, CIMMYT, Nairobi, Kenya;
    Email: b.m.prasanna@cgiar.org
  • Availability of seed material of the promising lines and pre-commercial hybrids, please contact:
    Dr. Mosisa Regasa (m.regasa@cgiar.org) if your institution is based in eastern Africa, or Dr. James Gethi (j.gethi@cgiar.org) if your institution is based in southern Africa or outside eastern and southern Africa.

Stepping up the fight against maize lethal necrosis in Eastern Africa

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MLN-Eastern-Africa1“I can now identify with accuracy plants affected with maize lethal necrotic disease,” stated Regina Tende, PhD student attached to CIMMYT, after attending the CIMMYT-Kenya Agricultural Research Institute (KARI) “Identification and Management of Maize Lethal Necrosis” workshop in Narok, Kenya, during 30 June-3 July 2013. This was not the case a few weeks ago when Tende, who is also a senior research officer at KARI-Katumani, received leaf samples from a farmer for maize lethal necrosis (MLN) verification.

Tende is one of many scientists and technicians who experienced difficulty in differentiating MLN from  other diseases or abiotic stresses with similar symptoms. According to Stephen Mugo, CIMMYT Global Maize Program (GMP) principal scientist and organizer of the workshop, this difficulty encouraged CIMMYT and KARI to organize this event to raise awareness about MLN among scientists, technicians, and skilled field staff; provide training on MLN diagnosis especially at field nurseries, trials, and seed production fields; train on MLN severity scoring to improve the quality of data generation in screening trials; and introduce MLN management in field screening sites to scientists, technicians, and skilled staff. The workshop brought together over 80 scientists and technicians from CIMMYT, KARI, and other national agricultural research systems (NARS) partners from Tanzania, Uganda, Rwanda, and Zimbabwe.

“It is important that all the people on the ground, particularly the technicians who interact daily with the plants and supervise research activities at the stations, understand the disease, are able to systematically scout for it, and have the ability to spot it out from similar symptomatic diseases and conditions like nutrient deficiency,” stated GMP director B.M. Prasanna.

Proper and timely identification of the MLN disease, which is a pre-requisite for effective control, is not easy. CIMMYT maize breeder Biswanath Das explains: “First of all, the disease is caused by a combination of two viruses, Maize chlorotic mottle virus (MCMV) and Sugarcane mosaic virus (SCMV). Secondly, its symptoms –severe mottling of leaves, dead heart, stunted growth (shortened internode distance), leaf necrosis, sterility, poor seed set, shriveled seeds– are not always unique to MLN but could be due to other fungal diseases and abiotic conditions.”  The training workshop was one of CIMMYT/KARI initiatives to combat the disease threatening all the gains made so far in maize breeding. “With nearly 99% of the commercial maize varieties so far released in Kenya being susceptible to MLN, it is important that institutions like CIMMYT and KARI, in strong collaboration with the seed sector, develop and deploy MLN disease resistant varieties in an accelerated manner,” stated Prasanna. One of the key initiatives in this fight is the establishment of a centralized MLN screening facility under artificial inoculation for Eastern Africa at the KARI Livestock Research Farm in Naivasha. Plans are also underway to establish a network of MLN testing sites (under natural disease pressure) in the region to evaluate promising materials from artificial inoculation trials in Naivasha. The state of the art maize doubled haploid (DH) facility currently under construction in Kiboko will also play a crucial role in accelerating MLN resistant germplasm development. “The DH technology, in combination with molecular markers, can help reduce by half the time taken for developing MLN resistant versions of existing elite susceptible lines,” stated Prasanna.

MLN-Eastern-Africa2During his opening speech, Joseph Ng’etich, deputy director of Crop Protection, Ministry of Agriculture, noted that about 26,000 hectares of maize in Kenya were affected in 2012, resulting in an estimated loss of 56,730 tons, valued at approximately US$ 23.5 million. Seed producers also lost significant acreages of pre-basic seed in 2012: Agriseed lost 10 acres in Narok; Kenya Seed lost 75; and Monsanto 20 at Migtyo farm in Baringo, according to Dickson Ligeyo, KARI senior research officer and head of Maize Working Group in Kenya.

While this loss represents only 1.7%, Ligeyo assured everyone that Kenya is not taking any chances and has come up with a raft of measures and recommendations: farmers in areas where rainfall is all year round or maize is produced under irrigation are advised to plant maize only once a year; local quarantine has been enforced and farmers are to remove all infected materials from the fields and stop all movement of green maize from affected to non-affected areas; seed companies must ensure that seeds are treated with appropriate seed dressers at recommended rates, they must also promote good agricultural practices, crop diversification, and rotation with non-cereal crops.

Throughout the workshop, participants learned about theoretical aspects of MLN, such as the disease dynamics, management of MLN trials and nurseries, and identification of germplasm for resistance to MLN. They also participated in practical sessions on artificial inoculation, and identification and scoring. Several CIMMYT scientists played an active role in organizing the workshop, including breeders Stephen Mugo, Biswanath Das, Yoseph Beyene, and Lewis Machida; entomologist Tadele Tefera; and seed systems specialist Mosisa Regasa. They were accompanied by KARI scientist Bramwel Wanjala, KEPHIS regulatory officer Florence Munguti, and NARS maize research leaders Claver Ngaboyisonga (Rwanda), Dickson Ligeyo (Kenya), Julius Serumaga (Uganda), and Kheri Kitenge (Tanzania). During his closing remarks, KARI Food Crops program officer Raphael Ngigi, on behalf of KARI director, urged participants to rigorously implement what they had learnt during the workshop in their respective countries or Kenya regions to help combat MLN at both research farms and farmers’ fields.

Commenting on the usefulness of the workshop, technical officer at KARI-Embu Fred Manyara stated: “I will no longer say I do not know or I am not sure, when confronted by a farmer’s question on MLN.”

Boosting the seed business through management training in Rwanda

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One of the major factors contributing to low national average maize yield in Rwanda is small-scale farmers’ limited access to improved maize seed, as was stated by Claver Ngaboyisonga (Crop Research and Extension at Rwanda Agricultural Board (RAB) director), Nyiringabo Ignace (Win Win Agri-tech Ltd director), and Ngoga Claudien (Agriculture Promotion – Sarura Seed Company director) during a Seed Business Management training organized by Win Win Agritech Ltd and CIMMYT from 4 to 6 September 2012 at the Pastoral Centre in Kigali, Rwanda. The training aimed to increase awareness on quality seed production and marketing, and to improve the skills and knowledge among new small seed companies, as quality seed production and marketing are challenges the sector is yet to overcome.

Maize cultivation in Rwanda is rapidly expanding due to changes in cropping systems, agricultural policies, and use of inputs such as fertilizer and new maize varieties adapted to different agro-ecologies. However, the national average yield remains low at 2.4 t/ha, despite the increase in area under maize cultivation from 109,400 ha in 2005 to 223,414 ha in 2011 and in production from 97,251 t in 2005 to 525,679 t in 2011. According to Ngaboyisonga, public-private partnership and involvement of private seed companies in seed production and marketing of quality improved seed is crucial for increasing the national average yield.

To achieve this goal, 28 participants from Win Win Agri-tech Ltd, Agriculture Promotion Company Ltd- Sarura, and RAB participated in the training program covering all aspects of the seed business, spanning from variety identification to marketing. The sessions were coordinated by CIMMYT seed systems specialists Mosisa Worku Regasa and James Gethi. Maereka Enock Kuziwa, a course participant, expressed satisfaction with the way the program was organized and conducted. “Seed business is a relatively new field in the Rwandan market and continuing support from CIMMYT to the seed business industry in Rwanda would be very useful,” added Ignace. This training will go a long way in promoting the use of improved maize varieties, especially those developed by CIMMYT in collaboration with RAB.

Scientists from SIMLESA Spillover countries visit Kenya

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IMG_6001A delegation of scientists from South Sudan, Rwanda, and Uganda —the spillover countries of the Sustainable Intensification of Maize-Legume Systems for Food Security in Eastern and Southern Africa (SIMLESA) initiative— visited Embu, Kenya, during 18-20 July 2012, to gain hands-on experience in implementing the program and to learn about its impact on livelihoods of smallholder farmers.

The delegation comprised Leonidas Dusengemungu, Albert Ruhakana, and Alphonse Nyobanyire from Rwanda; Luka Atwok, Anna Itwari, and Cirino Oketayoyt from South Sudan; and Drake N. Mubiru, William Nanyenya, and Godfrey Otim from Uganda. The scientists found the visit very educative and informative. They learned about the implementation of SIMLESA in Kenya and the role of national agricultural research institutions, the Kenya Agricultural Research Institute (KARI) in particular, in the process. They also gained insights into the innovation platform establishment and arising challenges, maize-beans intercropping and timing, challenges and coping strategies for the implementation of SIMLESA, and the level of adaptation of SIMLESA technologies in Kenya.

In his introductory remarks, Stephen Njoka, KARI-Embu Center Director, explained the Center’s mandate, activities, opportunities, and challenges in conducting agricultural research in Kenya. He noted that research programs at KARI-Embu range from food crops and crop health, natural resource management, horticultural and industrial crops, animal production and health research, outreach and partnerships, to cross-cutting programs, such as socioeconomics and applied statistics. The Center also offers advisory services, such as technical support for partners and capacity development for other service providers, including extension service providers and NGOs. Alfred Micheni, KARI Agronomist and SIMLESA Site Coordinator for eastern Kenya, explained that the western and eastern sites in Kenya had been selected because of their potential for the highest impact. This was determined by their agricultural production constraints: low soil fertility, erratic rainfall, high cost of farm inputs, high incidences of pests and diseases, high cost of credit, and small land sizes.

The scientists had a chance to interact with farmers participating in SIMLESA during a farmers’ field day in Kyeni Division, Embu County, on 20 July 2012. The field day was hosted by the Kyeni Innovation Platform and showcased various treatments under conservation agriculture (CA): maize-legume intercrop, minimum tillage, furrows and ridges, use of herbicides, residue retention, and variety selection. The scientists also had the opportunity to compare the robust crops under CA with those under conventional agricultural practices which were unable to cope with the prolonged drought in the region.

Charles Nkonge, SIMLESA National Coordinator, stressed the importance of the innovation platforms used by the program to evaluate and scale out maizelegume intensification technologies and knowledge in a participatory manner. Other strategies for scaling out include farmer exchange visits and participatory exploratory demonstrations. Nkonge stressed the importance of the participatory nature of the evaluations; through cooperation between the farmers and other collaborators, experimental designs of some of the treatments were adjusted to achieve more efficiency. For instance, the design of maize-pigeon pea intercropping trial was changed from intercropping of one pigeon-pea row between two rows of maize, giving one crop of maize and pigeon pea per season, to five rows of maize between two rows of pigeon pea and one row of common beans between two rows of maize. With the new design, two crops of maize, two crops of beans, and one crop of pigeon pea are harvested every year. This demonstration of successful practices allowed the representatives from SIMLESA Spillover countries to leave Kenya feeling optimistic about the new partnership.