The maize lethal necrosis (MLN) artificial inoculation screening site in Naivasha, Kenya will begin its second screening cycle of 2017 at the end of October, interested organizations from both the private and public sectors are invited to send maize germplasm for screening.
MLN was first discovered in Kenya in 2011 and quickly spread to other parts of eastern Africa; the disease causes premature plant death and unfilled, poorly formed maize cobs, and can lead to up to 100 percent yield loss in farmers’ fields.
CIMMYT and partners are dedicated to stopping the spread of this deadly maize disease by effectively managing the risk of MLN on maize production through screening and identifying MLN-resistant germplasm. The MLN screening facility supports countries in sub-Saharan Africa to screen maize germplasm (for hybrid, inbred and open pollinated varieties) against MLN in a quarantined environment.
This is the largest dedicated MLN screening facility in East Africa. Since its inception in 2013, the facility has evaluated more than 120,000 accessions from more than 15 multinational and national seed companies and national research programs.
More information about the disease and resources for farmers can be found on CIMMYT’s MLN portal.
Please note that it can take up to six weeks to process imports and clear shipments.
For assistance in obtaining import permits and necessary logistics for the upcoming screening, please contact:
L.M. Suresh
Tel: +254 20 7224600 (direct)
CIMMYT–Kenya, ICRAF House
United Nations Avenue, Gigiri
P.O. Box 1041–00621
Nairobi, Kenya.
Monica Mezzalama, head of CIMMYT’s Seed Health Laboratory. Photo: Xochiquetzal Fonseca/CIMMYT.
Breaking Ground is a regular series featuring staff at CIMMYT
EL BATAN, Mexico (CIMMYT) — At the International Maize and Wheat Improvement Center (CIMMYT) it all starts with a seed. Each year, the non-profit receives requests and sends more than 700,000 packets of seed to researchers, agricultural organizations and farmers around the world from its headquarters near Mexico City. These seeds stand up to climate change, produce higher yields with fewer resources and provide the nutrition a growing global population needs.
However, before each seed travels across an international border, it is essential to ensure that each one has a clean bill of health, free from virus, fungus and bacteria pathogens. Infected seeds must be controlled or there is a risk that plant pathogens will spread, affecting crop health and potentially threatening food security.
That is where plant pathology expert Monica Mezzalama, head of CIMMYT’s Seed Health Laboratory, gets involved.
“Seed movement around the world is regulated to limit the spread of pathogens across international borders,” said the senior scientist. “I coordinate and supervise seed health testing to ensure all seeds that pass through CIMMYT meet these international standards and do not pose a risk.”
Securing the health of seeds ensures that researchers, breeders and partner organizations don’t encounter infected seed and is essential to maintaining efficient agricultural research that has impact, she added.
Since taking the helm of the Seed Health Laboratory 15 years ago, all seed that has been inspected on its way out of CIMMYT must meet certification. If unhealthy seed is found it must be quarantined and destroyed under the law, explained Mezzalama.
Seeds arriving from partner organizations, researchers or farmers are also tested for disease and granted a “seed release” by Mezzalama and her team. Authorized seed then moves on to CIMMYT researchers to be studied for disease resistance, heat tolerance and micronutrient content and added into international breeding programs. Others are placed in the maize and wheat germplasm bank, where over 175,000 different varieties are preserved on behalf of humanity and are freely available to all upon request.
A curiosity for disease and a passion to cure led Mezzalama to a career as a plant pathologist. While studying for an undergraduate degree in agronomy in her hometown of Turin, Italy, she visited nearby vineyards to study plant pathogens for the first time.
“It was working in the vineyards where I first saw plant pathogens at work and where I saw the impact they have on farmers, and what it means for their livelihoods,” she said.
After graduating in 1986, Mezzalama began her first job at CIMMYT working alongside virologist Peter Burnett on a project dedicated to barley yellow dwarf (BYD) virus, which effects barley, wheat, maize, rice and other grasses worldwide. The experience opened her mind to a new world where she learned the inner workings of plant pathogens and started to study for a doctoral degree in plant pathology in Italy.
Since returning to CIMMYT in 2001, Mezzalama has led the Seed Health Laboratory, set institutional biosafety protocols to protect against harmful incidents, which include regular reviews of the biosafety in laboratory settings, as well as well as guidelines to follow, and participated in several research projects. Most recently, she joined a project to control the spread of Maize Lethal Necrosis (MLN), a devastating virus that poses a severe risk to food security in eastern Africa.
The complex disease results from the infection of two deadly viruses, maize chlorotic mottle virus and sugar cane mosaic virus. It spreads through infected maize seed and insect pests. Mezzalama’s skill in plant pathology detection was called upon to organize the opening of seed health laboratories in Kenya and Zimbabwe and also train staff on how to detect seed infected with MLN or the two associated viruses.
Currently, Mezzalama is in the final stages of developing a standard of detection protocol, providing the agriculture industry with knowledge of best practices and affordable tools to detect MLN infected maize seed.
“There are several products and methods that may be used for MLN detection in seed, these must be tested to see which obtain the most accurate results efficiently while taking into price into account,” she said.
Accuracy, time and cost are important factors when developing MLN detection protocols as common practice, implemented by partners in Kenya and other impacted countries, she explained.
Key donors to CIMMYT’s efforts in controlling MLN include the CGIAR Research Program on Maize (MAIZE), the United States Agency for International Development (USAID), the Bill & Melinda Gates Foundation (BMGF), Syngenta Foundation for Sustainable Agriculture (SFSA), the Association for Strengthening Agricultural Research in Eastern and Central Africa (ASARECA), the Alliance for a Green Revolution in Africa (AGRA), the Kenya Agriculture and Livestock Research Organization (KALRO), and the Rwanda Agriculture Board (RAB), CGIAR Fund Donors and other generous contributors to CIMMYT maize research.
CIMMYT wheat physiologist Matthew Reynolds describes the technology used for conducting research into heat and drought resilient wheat varieties in Ciudad Obregon in Mexico’s northern state of Sonora in March 2015. CIMMYT/Julie Mollins
EL BATAN, Mexico (CIMMYT) – Scientists involved in a major global initiative aimed at increasing wheat yields as much as 60 percent by 2050 got a recent boost when the U.S. government announced $3.4 million in new research funds.
Researchers affiliated with the International Wheat Yield Partnership (IWYP), focused on developing new high-yielding varieties of wheat to meet demand that will be generated by a projected 33 percent increase in population growth from 7.3 billion people today to 9.5 billion by 2050, will be eligible to apply for the grant money.
“This opens up new opportunities for scientists in the United States to provide invaluable input to the overall project of increasing yields, improving our potential to tackle this vital work to achieve global food security,” said Matthew Reynolds, wheat physiologist at the Mexico-based International Maize and Wheat Improvement Center (CIMMYT), one of the founding members of IWYP.
“Wheat is one of the world’s most important staple crops, providing a significant amount of daily calories and protein throughout the world,” Vilsack said.
“By 2050, the demand for wheat as part of a reliable, affordable, and nutritious diet will grow alongside the world population, and continued wheat research will play an important role in ensuring its continued availability.”
IWYP, which targets partner investments of up to $100 million, supports the G20 Wheat Initiative in its efforts to enhance the genetic component of wheat yield and develop new wheat varieties adaptable to different geographical regions and environments.
CLIMATE RISKS
Wheat yields face threats from global warming. Findings in a report from the Intergovernmental Panel on Climate Change (IPCC) state that it is very likely that heat waves will occur more often and last longer throughout the 21st century and rainfall will be more unpredictable.
Mean surface temperatures could potentially rise by between 2 to 5 degrees Celsius or more, the report said.
“Wheat currently provides 20 percent of calories and protein consumed worldwide and current models show that a 2 degree increase in temperature would lead to 20 percent reduction in wheat yield and that a 6 degree increase would lead to a 60 percent reduction,” Reynolds said.
“If we have a 40 percent yield reduction due to climate change, the risks to food security will be increased because wheat production has to increase by 60 percent just to keep up with population projections.”
In addition to CIMMYT, IWYP members include Britain’s Biotechnology and Biological Sciences Research Council (BBSRC), Mexico’s Secretariat of Agriculture, Livestock, Rural Development, Fisheries and Food (SAGARPA), the U.S. Agency for International Development (USAID), the Grains Research and Development Corporation of Australia (GRDC), the Department of Biotechnology of India (DBT), Agriculture and Agri-Food Canada (AAFC), the Institut National de la Recherche Agronomique (INRA) in France and the Syngenta Foundation for Sustainable Agriculture (SFSA) in Switzerland.
Applications are due May 3, 2016 and more information is available via the NIFA-IWYP request for applications.
Direct seeding of maize using a two-wheel tractor has been made possible over the past decade or so by manufacturing companies in China, India, and Brazil (among others) that produce commercially available seeders. Several of these seeders have been tested for the past two or three years in Ethiopia, Kenya, Tanzania, and Ethiopia under the Farm Mechanization and Conservation Agriculture for Sustainable Intensification (FACASI) project supported by the Australian International Food Security Research Center (AIFSRC).
One of the best performing commercially available seeders (in terms of field capacity, precision in seed rate and planting depth, crop emergence, etc.) is manufactured by the Brazilian company Fitarelli. However, this seeder is expensive (above US$ 4,000), difficult to maneuver (especially in small fields), and lacks versatility (minimum row spacing is 80 cm).
In response, several initiatives have aimed at producing toolbar-based seeders to be manufactured locally and cheaply, that could be used in different configurations (to seed one, two, or more rows) and could perform other operations (such as forming planting beds). One such toolbar is the Gongli seeder, which is well suited to sow small grain crops such as wheat and rice in Asian fields, but not maize under typical field conditions in Africa. Two years ago, Jeff Esdaile, inventor of the original Gongli, and Joseph Mutua, from the Kenya Network for Dissemination of Agricultural Technologies, produced a modified version of the Gongli – the Gongli Africa + thanks to funding from CRP MAIZE (as reported in Informa No. 1862). In parallel, another toolbar using a different design was produced by Jelle Van Loon and his Smart Mechanization/Machinery and Equipment Innovation team at CIMMYT-Mexico.
Both the Gongli Africa + and the Mexican toolbar have their strengths and their weaknesses. Both have also been judged as too heavy by local service providers. Thus, CRP MAIZE and the Syngenta Foundation for Sustainable Agriculture co-funded a two-week session (8-27 October) in Zimbabwe to develop a “hybrid toolbar” having the strengths of both the Gongli Africa + and the Mexican toolbar but weighing under 100 kg. Jeff Esdaile, Joseph Mutua, and Jelle Van Loon spent the entire two weeks manufacturing three prototypes of the hybrid at the University of Zimbabwe. The two-week session also served as hands-on training for staff of three of Zimbabwe’s major manufacturing companies of agricultural equipment (Zimplow LTD, Bain LTD, and Grownet LTD) as well as representatives of the informal sector.
The hybrid toolbar is expected to sell for a quarter of the price of a Fitarelli seeder, although its performance (in terms in term of field capacity, fuel consumption, precision, and crop emergence) is expected to be equivalent. Its weight suits the needs of local service providers better and it is infinitely more versatile (several configurations are possible depending on the desired row spacing, soil conditions, the amount of mulch, etc.). The hybrid toolbar will be thoroughly tested in Zimbabwe during the coming months. A prototype will be shipped to Bangladesh and another to Mexico for further testing and to share the design.
A Fitarelli seeder is good at establishing a maize crop under no-till conditions, but expensive, difficult to operate in small fields, and heavy. Photo: Frédéric Baudron
The first hybrid toolbar being tested at CIMMYT-Harare. It is cheap, easy to maneuver, light, and versatile. Three local companies and informal sector representatives have been trained to manufacture it locally. Photo: Frédéric Baudron
PLC6 is a term used to refer to an advanced stage of hybrid testing at Syngenta, a partner of the Affordable, Accessible, Asian (AAA) Drought Tolerant Maize Project. Four hybrids, representing combinations of Syngenta and CIMMYT germplasm are currently at PLC6 in big plots at multiple locations. The trajectory of this process points to pilot marketing of a limited quantity of hybrid seed in 2016 and a full market launch in 2017.
AAA Drought Tolerant Maize Project Meeting, ICRISAT Campus, Hyderabad, India. 22-23 July 2015. Photo: P.S. Rao/ICRISAT
Four million hectares in India and Indonesia is the potential target area of this project. This translates to a market potential of about 80,000 metric tons of seed and offers the opportunity to address the needs of over five million households. In Indonesia, this primarily covers the island of Sulawesi and eastern Java province. In India, the targeted west central zone includes drought prone and tribal areas, a high risk environment where farmers require improved low-cost seed.
According to Syngenta, the region’s climate and other dynamics make seed marketing risky, unpredictable and unattractive, and is often overlooked by the private seed sector – exactly the kind of underserved area CIMMYT is mandated to target.
The AAA annual meeting was held at the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) campus in Hyderabad, India on 22 and 23 July 2015. Members of the AAA team highlighted achievements over the past five years that ranged from identifying hybrid combinations, fast tracking them to deployment, developing new inbred lines, identifying molecular marker leads for grain yield under drought and for root traits, generating information on genomic selection and genome wide associations and building human, infrastructural, informatics and networking capacity. All this was done through an exploratory partnership model that included NARS partners (from Vietnam and Indonesia) in addition to Syngenta.
CIMMYT and the AAA team would like to thank the Syngenta Foundation for Sustainable Agriculture (SFSA), especially Mike Robinson, Chief Science Advisor and the mastermind behind this approach, for the support provided and for enabling such a collaborative opportunity.
CIMMYT wishes to announce that the start of the planting season for the 2015A planting season at the KALRO–CIMMYT maize lethal necrosis (MLN) artificial inoculation screening site at Naivasha, Kenya. Interested organizations from both the private and public sectors are invited to send their maize germplasm for screening. Planting is due to start at the end of May 2015 following an upgrade of the current irrigation system. Please note that it can take up to six weeks to process imports and clear shipments..
The MLN Screening Facility is the largest of its kind established in response to the MLN outbreak in eastern Africa in 2013. It supports countries in sub-Saharan to screen maize germplasm (hybrids, inbreds, and open pollinated varieties) against MLN in a quarantined environment. The facility is managed jointly by the Kenya Agricultural and Livestock Research Organization (KALRO) and CIMMYT, and was established with support from the Bill & Melinda Gates Foundation and the Syngenta Foundation for Sustainable Agriculture. Since its inception in 2013, the facility has evaluated more than 20,000 accessions from more than 15 multinational and national seed companies and national research programs.
For assistance in obtaining import permits and necessary logistics for the upcoming screening, please contact Biswanath Das
Tel: +254 20 7224600 (direct)
CIMMYT–Kenya, ICRAF House, United Nations Avenue, P.O. Box 1041–00621, Nairobi, Kenya.
Policies designed to promote maize industry growth require data and information, which is often difficult to obtain in Asian countries. This was discussed during the technical session on improving maize seed systems in Asia at the 12th Asian Maize Conference. David Spielman, senior research fellow at the International Food Policy Research Institute (IFPRI), highlighted that policy-makers often face difficult challenges in promoting seed industry growth – especially in Asian countries that have more smallholder and resource-poor farmers.
Spielman said, “Innovation policies require data on firm-level research and development spending; product pipeline and competition policies require data on market structure and firm behavior.”
Firms often do not share proprietary revenue data and governments may not monitor firm-level activity on a regular basis. One of the factors could be that policy-makers are not sufficiently informed about the opportunities and trade-offs associated with designing laws and regulations that enable the effective governance of seed industry development. Spielman emphasized that a better designed dataset with a finite set of indicators to measure competition and innovation in a country’s seed industry can better inform policy-makers.
The conference highlighted the need for the public and private sectors to work together to provide affordable new seed varieties and deliver new technologies to smallholder farmers. An eminent group of panelists – Arvind Kumar, Rasi Seeds; Shilpa Divekar Nirula, Monsanto; Fan Xingming, Yunnan Academy of Agricultural Sciences, China; John McMurdy, U.S. Agency for International Development; and Bijendra Pal, Bioseed, discussed the opportunities and challenges to ensure a vibrant Asian maize seed sector through public-private partnerships (PPPs).
The panel noted that decision-makers should not look at public vs. private; rather they should learn from models and best practices where the two sectors have worked together successfully.
As a best practice on PPPs, Ian Barker, head of agricultural partnerships at the Syngenta Foundation for Sustainable Agriculture (SFSA), talked about its Seeds2B program in Africa that builds linkages between breeders and seed companies to make more improved seed varieties available to farmers at the right time and price.
He also highlighted that SFSA is now aiming to kick- start the Seed2B concept in Asia – bringing together breeders, seed companies, farmer associations and other relevant players in the Asian maize value chain – to improve access to seed in marginal maize areas. Barker said, “Public-private breeding partnerships can efficiently deliver new affordable and accessible hybrids – correctly positioned and targeted at proven smallholder demand.”
Partners of the Maize Lethal Necrosis (MLN) project in Africa, funded by the Bill & Melinda Gates Foundation and Syngenta Foundation for Sustainable Agriculture (SFSA), came together to review and discuss the progress and next steps for the project at a workshop on 14-15 May.
“The primary goal of this project is to identify MLN-tolerant germplasm, including inbred lines and pre-commercial hybrids, for potential deployment of MLN-tolerant varieties (with other relevant adaptive traits) through partners in Sub-Saharan Africa. The project also seeks to gain information regarding the genetics of resistance to the disease, create awareness and build capacity for more effective MLN management.
Group photograph taken at the MLN Screening Facility, Naivasha, Kenya. Photo: Florence Sipalla/CIMMYT
“As the project matures, we will be screening additional promising germplasm coming out of the MLN screening facility at Naivasha and from national agricultural research institute (NARI) partners at satellite testing centers in Kenya, Tanzania and Uganda under high natural disease pressure. This will help to validate the performance of the germplasm under MLN in important agro-ecologies,” said CIMMYT Global Maize Program Director and MLN-Africa project leader, BM Prasanna.
Mike Robinson, chief scientific advisor at SFSA and representatives of several seed companies and NARIs in Kenya, Tanzania and Uganda participated in the MLN Field Day at Naivasha on 14 May. The companies included East African Seed, Kenya Seed Company, Meru Agro, Monsanto, DuPont Pioneer and Seed Co Limited. “We hope to submit several germplasm entries for trials at the MLN screening facility in the next planting season,” said Francis Ndambuki, a maize breeder with Kenya Seed Company.
Less than a year after its opening, the MLN screening facility is now fully functional, evaluating materials from several partner organizations, including the International Institute for Tropical Agriculture, NARIs and seed companies. The partners/ visitors had an opportunity to see several trials taking place at the facility, as well as promising MLN-tolerant germplasm. Scientists involved in this project are working intensively – in collaboration with national and international institutions to develop and validate virus inoculation and germplasm screening protocols. They are also testing a large array of germplasm for responses against MLN under artificial inoculation, developing diagnostic techniques and building the capacity of national partners in MLN diagnostics and management.
The CIMMYT seed systems team is working on scaling-up the identified MLN-tolerant germplasm and distributing it to interested public and private partners in Africa. The progress made on the project’s diverse fronts was presented in detail, and the next steps were discussed during the project meeting held in Nairobi on 15 May. “We are pleased that CIMMYT teamed with KARI to address this important issue for Africa,” said Joseph Mureithi, KARI deputy director. Mureithi continued, “The MLN screening facility at Naivasha is a model of partnership between CGIAR centers, NARIs and the private sector,” adding that the results of the trials at the facility would have far-reaching impact. “Stresses such as drought and poor soil fertility could potentially compound the expression of the MLN disease,” said KARI virologist Dr. Anne Wangai, a key partner in this project. “This is a great opportunity to demonstrate the confluence of technologies – breeding, pathology and entomology,” said Robinson, urging the project partners to further strengthen the scientific edge and demonstrate how the research being undertaken on the disease can quickly result in products that benefit African farmers affected by the disease.
“Policy advocacy is key,” said Wilfred Mwangi, CIMMYT’s regional representative for Africa. He emphasized the need to fast-track registration and commercialization of the MLN-tolerant hybrids, especially in the MLN-affected countries. The Kenya Plant Health Inspectorate Service (KEPHIS) was commended for its commitment and support in fast-tracking the release of promising MLN-tolerant hybrids. “There is fast progress on the MLN research and the first wave of improved materials is coming now,” said Robinson, adding that he is looking forward to seeing more materials in the future. He emphasized the importance of coordination, policy clarity, communication and international synergies on MLN research and development in Africa.
There is a growing need for drought-tolerant maize cultivars among smallholder farmers in developing countries, particularly in Asia where the cultivars’ development is a technical reality and outputs are achievable. Public-private partnership projects such as the Affordable, Accessible, Asian Drought-Tolerant Maize (AAA) project attempt to address smallholder farmers’ urgent and long-lasting need to access available and affordable new crop varieties that are robust, drought-tolerant and high-yielding.
CIMMYT pathologist George Mahuku and MLN technician Janet Kimunye examine tassels for pollen production on an infected plant. MLN causes a symptom called ‘tassel blast’ where the tassels of infected plants do not shed or produce pollen. Photos courtesy of George Mahuku
By George Mahuku and Florence Sipalla/CIMMYT
Germplasm screening at the maize lethal necrosis (MLN) screening facility at the Kenya Agricultural Research Institute (KARI) Naivasha is underway, and CIMMYT pathologist George Mahuku said some inoculated lines are showing levels of resistance.
He described the green islands among the maze of yellow in the fields as a demonstration of the success of the testing protocols being used at the site. “This is the lifeline for farmers,” he said. “Next we will be incorporating genes from these lines into adapted germplasm and using the Doubled Haploid facility in Kiboko to quickly develop inbred lines with resistance to MLN.”The deadly maize disease was first identified in Kenya in 2011 and has since been diagnosed in the Democratic Republic of Congo, Rwanda, Tanzania and Uganda. The MLN screening facility was established in 2013 with funding from the Bill & Melinda Gates Foundation and the Syngenta Foundation for Sustainable Agriculture to serve maize breeding institutions in Sub-Saharan Africa in response to 2014 the emergence of the disease.
CIMMYT pathologist George Mahuku inspecting plants that show tolerance to MLN in Naivasha, Kenya.
“To date, we have planted more than 19,000 different types of germplasm on 15 hectares,” Mahuku said. “This germplasm was submitted by both private and public sector partners, including CIMMYT and the International Institute of Tropical Agriculture (IITA).”
All germplasm has been inoculated, Mahuku said, and symptoms are fully visible. Operations at the facility include maintaining pure strains of the viruses that cause MLN, producing inoculum for artificial inoculation, evaluating maize hybrids and inbred lines for response to MLN and building the capacity of stakeholders including scientists, technicians, farmers and extension workers to handle the disease.
The facility also provides employment opportunities for the community, hiring more than 30 people for activities such as weeding, irrigation and disease scoring. Because the facility screens germplasm from different countries, it’s isolated from farmers’ maize plots and certified as a quarantine site. “We still do not fully understand the variability in virus strains, whether the virus strains in Rwanda, Tanzania or Uganda are the same as the ones in Kenya,” said Mahuku.
After disease evaluations, all plant debris will be disposed of by incineration. The facility has received many visitors from universities, international organizations and public and private institutions. “There is a lot of interest in learning and knowing the disease,” Mahuku said.
It recently hosted two scientists from Ethiopia who will share the knowledge gained with their colleagues and will conduct surveys to assess whether the disease is in their country. “To see all this going well is breathtaking,” said CIMMYT technician Janet Kimunye, who is in charge of virus maintenance, inoculum production and inoculations in the field and has been involved in MLN research from the beginning, initially as a consultant to CIMMYT.
“We have assembled a really good team here; watching them work way into the night and weekend is heartening,” said Mahuku. “Everybody wants a solution to this problem that is threatening their food security.”
One of the greenhouses where artificial inoculum is produced and multiplied for research purposes.
Facts about the Facility:
Area planted: 15 ha
Number of rows: 49,500
Total germplasm: 19,539
Inoculated area: 6.5 ha
Disease expression: 4.5 ha is under disease evaluation as symptoms are expressing well
The Insect Resistant Maize for Africa (IRMA) project received praise for significant progress on field and post-harvest insect pest research at its conclusion last month.
“Several new maize hybrids and open pollinated varieties with substantial insect resistance have been produced that will greatly benefit maize growers in eastern and southern Africa,” said Mike Robinson, program officer for the Syngenta Foundation for Sustainable Agriculture (SFSA) at the IRMA End-of-Project Conference in Nairobi, Kenya, from 24-26 February. Robinson congratulated CIMMYT and project partners and wished the participating organizations continued success. The purpose of the conference was to share experiences, achievements and lessons from IRMA III and discuss future prospects in the release, dissemination and use of insect-resistant maize in eastern and southern Africa.
It drew more than 80 participants from CIMMYT, national agricultural research systems, national universities, donors and the seed industry. The Developing Maize Resistant to Stem Borer and Storage Insect Pests for Eastern and Southern Africa project, known as IRMA III Conventional Project 2009-2013, was managed by CIMMYT and funded by SFSA. Building on progress and breakthroughs of IRMA I and II, IRMA III contributed to food security by developing and availing field and post-harvest insect-resistant maize varieties in Ethiopia, Kenya, Malawi, Mozambique, Tanzania, Uganda, Zambia and Zimbabwe.
(Photo: Wandera Ojanji)
Collectively, these countries produce about 26 million tons and consume 32 million tons of maize annually. Relatively low maize productivity in the countries – about 1.3 tons per hectare (t/ha) compared to 4.9 t/ha worldwide – can be attributed in part to stem borers, according to Stephen Mugo, CIMMYT principal scientist and IRMA project leader. Stem borers destroy as much as 15 percent of maize crops, while maize weevils and the larger grain borer destroy 20 to 30 percent of harvested maize. Hugo De Groote, CIMMYT economist, estimated the losses from stem borers at 13.5 percent, or 4 million tons, and those from storage pests at 11.2 percent, or 3.5 million tons, with the total value of these losses estimated at just over US$1 billion in the region. “Addressing the challenges that farmers face in producing and storing maize is vital to the future food security of the region,” Robinson said. “Minimizing such losses in an economically sustainable way will significantly contribute to nutrition and food security.”
IRMA III addressed these challenges through identification and commercial release of major insect-resistant maize cultivars; identification of new germplasm sources of resistance to stem borer and post-harvest insect pests among landraces, open pollinated varieties (OPVs) and CIMMYT lines (CMLs); and development of new insect-resistant germplasm. Kenya released 13 stem borer-resistant (SBR) conventional maize varieties (three OPVs and 10 hybrids) and four storage pest-resistant (SPR) hybrids. Kenya has also nominated nearly 10 stem borer- and four postharvest- resistant hybrids to national performance trials.
Three insect-resistant varieties —two hybrids (KH 414-1 SBR and KH 414-4 SBR) and one OPV (Pamuka) – were commercialized in Kenya by Monsanto, Wakala Seeds and the Kenya Agricultural Research Institute Seed Unit. De Groote estimated the annual value of project benefits at between US$ 19 million and US$ 388 million. He put the benefit-cost ratio at 94 in the optimistic scenario, meaning that for each dollar the project spent, farmers would gain 95, indicating very good returns.
“These results justify the important role that breeding for resistance could play in reducing maize losses, and the high potential returns to such programs in the future,” De Groote stated. Looking to the future, Mugo emphasized the need to ensure farmers have access to the insect-resistant varieties. “We must, from now on, engage in variety dissemination and commercialization of the new SBR and SPR varieties,” Mugo said. “We need a more targeted breeding program that incorporates drought, nitrogen use efficiency and maize lethal necrotic disease tolerance.”
CIMMYT is leading collaborative research efforts to control the deadly maize lethal necrosis (MLN) disease that is devastating crops in Kenya, Rwanda, Tanzania and Uganda. First identified in farmers’ fields in eastern Africa in 2011, MLN results from the combined infection of two plant viruses and can cause nearly 100 percent crop loss.
Surveillance, avoiding seed movement from disease-prone areas, instituting maize-free seasons and the development and use of resistant maize varieties can help prevent its spread. CIMMYT is spearheading efforts to identify sources of MLN resistance, developing a strategy to contain the disease and studying the disease and insects that contribute to its spread.
In addition, CIMMYT has produced fact sheets and videos to raise awareness on MLN, in addition to organizing meetings and workshops to train partners from national research programs in eastern Africa on how to identify the disease and curb its spread. “We have to come together to stop the disease, as it affects food security for those who depend on maize,” said CIMMYT pathologist George Mahuku during a Seed Trade Association of Kenya (STAK) congress from 6 to 8 November. “Developing MLN-resistant varieties is the most cost-effective way to deal with the disease.” He also said the recently-launched CIMMYT-Kenya Agricultural Research Institute MLN Screening Facility and Maize Doubled Haploid Facility would help speed development of MLN-resistant varieties.
The MLN facility was established with funding support from both the Bill & Melinda Gates Foundation and the Syngenta Foundation for Sustainable Agriculture while the DH Facility was established with funding from the Bill & Melinda Gates Foundation. During the three-day event, issues that affect the seed sector – including MLN – were addressed. Mahuku described the disease and research efforts to control it, while dispelling fears about its transmission through seed. “Seed movement is crucial for us to increase productivity and widen our genetic base, but the seed should be clean, produced in MLN-free areas and certified MLN-free to minimize accidental introduction of MLN viruses,” he said. Mahuku stressed the need for more research on the disease’s seed transmission rate and alternative hosts.
CIMMYT will partner with the International Centre of Insect Physiology and Ecology (ICIPE) to study transmission of MLN through aphids, beetles and thrips, as well as their interactions with the MLN viruses. Mahuku also urged seed companies to recognize MLN symptoms and notify scientists if they spot them on their farms. Scientists can then investigate and design and adopt appropriate strategies for combating it.
He encouraged stakeholders to practice maize-free seasons to reduce sources of infection. MLN has created an emergency for sectors caught unaware and without funding to immediately address the disease. “We encourage the [seed] industry to set aside some funds for these kinds of emergencies,” said Esther Kimani, general manager of phytosanitary services at the Kenya Plant Health Inspectorate Service (KEPHIS). Existing commercial hybrids in Kenya have already been tested for MLN, said STAK Executive Officer Evans Sikinyi during a field tour of Marula farm in Naivasha, where CIMMYT is testing a large selection of experimental and commercial varieties under MLN infection.
The participants also toured the Maize Lethal Necrosis Screening Facility in Naivasha and were invited to submit seed for screening at the facility. Mahuku also called for the standardization of screening protocols. “If we are speaking the same language, we will move far,” he said. “We need to develop and use standardized protocols to ensure that the same material can be planted in 10 different areas in different countries for screening purposes, and this information can be harmonized.”
Participants will carry the messages of progress, hope and caution to colleagues, partners and farmers back home. “We will use lessons learned on this trip to strengthen surveillance of the disease in South Sudan,” said Cirino Oketayot, head of the research unit in South Sudan’s Ministry of Agriculture, Forestry, Tourism, Animal Production, Fisheries, Cooperatives and Rural Development. Added Gloria Ngila, the general manager of Dryland Seeds: “This helps one realize how much CIMMYT is working with the government to alleviate the problem.” The STAK congress was attended by seed sector representatives from Burundi, India, Kenya, Malawi, Rwanda, South Africa, South Sudan and Zimbabwe.
Partners from 13 countries working with the Drought Tolerant Maize for Africa (DTMA) project said they benefitted from its help during the 2012-13 crop season. DTMA trained maize breeders and technicians, rehabilitated seed storage facilities, supported research institutes and seed companies to release varieties and produce breeder seed and began hybrid seed production in places where seed companies did not exist. Project partners from eastern, southern and West Africa met in Nairobi, Kenya, from 23 to 27 September for DTMA’s annual meeting. They discussed progress made in developing and deploying drought-tolerant maize varieties to benefit smallholder farmers in Africa. Maize varieties that respond to climate change challenges – such as drought and infrequent or unevenly distributed rainfall – are key in helping those who depend primarily on rain-fed agriculture. The DTMA project is funded by the Bill & Melinda Gates Foundation.
Officials who opened the meeting included Thomas Lumpkin, director general of CIMMYT; Ylva Hillbur, deputy director general of the International Institute for Tropical Agriculture (IITA); Ephraim Mukisira, director of the Kenya Agricultural Research Institute (KARI); and Joseph De Vries, director of the Alliance for a Green Revolution in Africa (AGRA) Program for Africa’s Seed Systems (PASS). DTMA has released 140 drought-tolerant maize varieties since 2007, including 81 hybrids and 59 open-pollinated maize varieties. These varieties perform well under drought stress as well as adequate rainfall. “Over the last seven years, DTMA has made significant progress in developing and delivering improved technologies,” Lumpkin said. “Farmers have also benefited from [drought-tolerant] varieties that possess other desirable traits such as resistance to major diseases such as maize streak virus and gray leaf spot,” he added. Mukisira highlighted the partnership between CIMMYT and KARI. KARI centers in Embu, Kakamega and Kiboko, Kenya are part of the drought screening network and the organization’s socioeconomics team is working with DTMA on household surveys across the country. Mukisira said the collaboration produces evidence-based research findings that help inform and engage policy makers. CIMMYT and Kari recently opened two major maize research facilities in Kenya.
Meeting participants participated in the openings, which were inaugurated by Felix Koskei, Kenya’s Cabinet Secretary for Agriculture, Livestock and Fisheries and Sicily Kariuki, principal secretary for the Kenyan Agriculture, Livestock and Fisheries Ministry. Lumpkin thanked DTMA donors, especially the Bill & Melinda Gates Foundation, for supporting DTMA as well as the new maize doubled haploid and maize lethal necrosis screening facilities, the latter of which is co-funded by the Syngenta Foundation for Sustainable Agriculture. The national agricultural research system and small- to medium-sized seed companies working with CIMMYT maize projects such as DTMA will be key beneficiaries of these facilities.
Mukisira recognized the role seed companies play in deploying drought-tolerant seed for market and making it accessible to smallholder farmers. “We encourage you to continue investing in the marketing and promotion of improved droughttolerant maize varieties,” he said. Hillbur said the strong partnerships DTMA has built with the national agricultural research system and seed companies as well as the “top quality science approach involving the breeders, economists, social scientists and seed systems specialists” are two of the project’s distinguishing factors. DTMA Project Leader Tsedeke Abate said the project is moving toward its goal of reaching more than 30 million farmers with drought-tolerant maize varieties by the end of 2016. The national agricultural research systems will be key players in breeding and disseminating improved varieties, he said. Moving forward, the project will continue to help mainstream drought-tolerant varieties, enhance seed systems partnerships with AGRA-PASS, build the capacity of the national agricultural research system to produce breeder seed, mainstream gender and build on socioeconomic research to provide evidence for policy advocacy.
Felix Koskei, Kenya’s Cabinet Secretary for Agriculture, unveils the plaque of the Doubled Haploid Facility in Kiboko, Makueni County. Looking on is Bodduppali Prasanna, director of CIMMYT’s Global Maize Program (Left), Thomas Lumpkin, director general of CIMMYT and Ruth Kyatha from the Makueni County Cabinet Secretary for Agriculture. Photo: Wandera Ojanji
CIMMYT, in partnership with the Kenya Agricultural Research Institute (KARI), established two major maize facilities in Kenya last week. The Maize Doubled-Haploid Facility for Africa at KARI-Kiboko aims to accelerate the development of stress-resilient and nutritionally-improved maize varieties while the Maize Lethal Necrosis Screening Facility at KARI-Naivasha will focus on tackling deadly maize lethal necrosis (MLN).
The doubled-haploid (DH) facility, established with funding support from the Bill & Melinda Gates Foundation, was inaugurated on 25 September by Felix K. Koskei, Kenya’s cabinet secretary for Agriculture, Livestock and Fisheries. Representatives from the ministry and country government, the CIMMYT board of trustees and management committee and the KARI director and board of management attended the inauguration. “Just as agriculture is the driver of economic growth, so is agricultural research the engine of agriculture,” Koskei said. “I take this opportunity to congratulate maize research scientists for their tireless efforts in conducting cutting-edge agricultural research directed at solving the constraints that hinder agricultural growth.”
The facility will help serve African agriculture for years to come, said CIMMYT Director General Thomas Lumpkin. “The Maize DH Facility will be key to fast-tracking the development and delivery of drought tolerant, disease and insectpest resistant and nutritionally enriched maize varieties for the benefit of Kenyan and African farmers at large,” he said. The MLN screening facility will address another challenge: while maize is Africa’s most important food crop, the 2011 drought in East Africa – combined with the emergence of MLN in eastern Africa in 2012 – resulted in significant crop losses and severe food shortages across the region. The accelerated development and delivery of MLNresistant maize varieties with other important adaptive traits is an urgent priority for CIMMYT and its partners in the region. The MLN Screening Facility at KARI-Naivasha is central to achieving this goal and was made possible with funding support from the Bill & Melinda Gates Foundation, and the Syngenta Foundation for Sustainable Agriculture.
Thomas Lumpkin waters a commemorative tree seedling he planted after the inauguration of the DH Facility in Kiboko. Photo: Wandera Ojanji
Inaugurating the MLN Screening Facility at KARI-Naivasha, Kenya’s Principal Secretary to the Ministry of Agriculture Sicily Kariuki commended CIMMYT and KARI for their rapid response to MLN and for establishing a screening facility that will benefit the entire region. The facility aims to provide MLN phenotyping services and effectively manage the risk of MLN on maize production through screening and identifying MLNresistant maize germplasm. It will make use of public and private sector research partners in Africa to contribute to food security and to the livelihoods of farming communities in Eastern Africa. Through the KARI-CIMMYT partnership, several promising maize inbred lines and hybrids with resistance to MLN have Photo: Florence Sipalla already been identified and are being further validated and used in breeding strategies to develop MLN-resistant maize hybrids. Prasanna Boddupalli, director of the CIMMYT Global Maize Program, emphasized the facility “will serve not only CIMMYT and KARI, but other interested public and private sector institutions that are engaged in developing and delivering improved maize varieties to farmers in Africa.”
A new facility at CIMMYT-Hyderabad, India, will allow researchers to assess and quantify key root traits and their dynamics under various growing conditions. CIMMYT’s new root phenotyping facility is based on the lysimetric system, by which scientists can directly assess and quantify root traits and their dynamics under various growing conditions. It also allows high-precision phenotyping of various root traits.
A high-profile delegation from Groupe Limagrain, led by its CEO, visits the newly-established root phenotyping facility at CIMMYT-Hyderabad, India. Photo: T. Durga/CIMMYT
The system revolutionizes the research, moving from a static assessment of roots through time-consuming extraction and scanning to a real-time measurement of water uptake, water use and an assessment of variation in roots under different growing conditions in the rhizosphere. Recent advances in high-precision weighing systems and information technology tools have greatly improved its efficiency and effectiveness as a root phenotyping system.
A representative from USAID observes maize root extracted from a minirhizotron. Photo: T. Durga/CIMMYT
CIMMYT’s root phenotyping facility is specially designed for – but not restricted to – maize. The facility features 2,400 minirhizotron observation tubes placed in eight concrete pits. A wheeled stand is used to lift the rhizotrons for weighing. The weight of the cylinder, along with the entire plant, is monitored periodically and allows researchers to estimate the amount of water used and transpired as well as the transpiration efficiency of different genotypes. Roots are critically important to plants because they are the part first exposed to any soil-mediated stresses, such as drought, waterlogging, salt stress or nutrient stress. Root traits govern the overall performance of plants; however, this important hidden half is often avoided due to the complexity involved in studying root structure and functions.
Mini-rhizotrons with maize plants sit at the root phenotyping facility. Photo: T. Durga/CIMMYT
In maize, the genotypic variation in root traits and variation under stresses can be carefully selected in targeted breeding for stress tolerance, which can contribute significantly to genetic gains. Root traits are often judged on the basis of related characteristics, which may not accurately explain the stress-responsive — or adaptive — structural and functional changes in roots under sub-optimal or stressed conditions. The facility is used in phenotyping root traits of mapping populations developed for various molecular breeding projects on drought and heat stress tolerance as well as other traits usually observed in field conditions, including morphological traits and grain yield. It is located under a renovated rain shelter, protecting the trials from rain at the targeted crop stage and allowing for yearround use.