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funder_partner: Mexico's Secretariat of Agriculture and Rural Development (SADER)

Breaking Ground: Scientist Deepmala Sehgal on the trail of novel wheat diversity

Breaking Ground is a regular series featuring staff at CIMMYT

Deepmala Sehgal, wheat geneticist and molecular breeder at CIMMYT. Photo: M. Listman/CIMMYT
Deepmala Sehgal, wheat geneticist and molecular breeder at CIMMYT. Photo: M. Listman/CIMMYT

EL BATAN, Mexico (CIMMYT) — Molecular analysis research by Deepmala Sehgal, a wheat geneticist and molecular breeder who joined the International Maize and Wheat Improvement Center (CIMMYT) as an associate scientist in 2013, has led to the discovery of novel genes for yield, disease resistance and climate resilience in previously little-used wheat genetic resources.

But getting to the point of applying cutting-edge DNA marker technology to support CIMMYT wheat breeding has involved a few dramatic moves for the New Delhi native, who studied botany throughout middle school and university. “I loved science and chose plant science, because I enjoyed the field trips and didn’t like dissecting animals,” Sehgal said, explaining her choice of profession.

It wasn’t until she was studying for her Ph.D. at Delhi University in 2008 that she first used molecular markers, which are DNA segments near genes for traits of interest, like drought tolerance, and which can help breeders to develop improved crop varieties that feature those traits.

“For my thesis, I used molecular markers in a very basic way to analyze the diversity of safflower species that the U.S. Department of Agriculture had in its gene bank but didn’t know how to classify. I found a place for some and, for several, had to establish completely new subspecies,” Sehgal said.

Later, as a post-doctoral fellow at the University of Aberystwyth in Britain, Sehgal used an approach known as fine mapping of quantitative trait loci (QTL), for drought tolerance in pearl millet. “The aim of fine mapping is to get shorter QTL markers that are nearer to the actual gene involved,” she explained, adding that this makes it easier to use the markers for breeding.

As it turned out, Sehgal’s growing proficiency in molecular marker research for crops made her suited to work as a wheat geneticist at CIMMYT.

“By 2013, CIMMYT had generated a huge volume of new data through genotyping-by-sequencing research, but those data needed to be analyzed using an approach called “association mapping,” to identify markers that breeders could use to select for specific traits. My experience handling such data and working with drought stress gave me an in with CIMMYT.”

Based at CIMMYT’s Mexico headquarters, Sehgal currently devotes 70 percent of her time to work for the CIMMYT global wheat program and the remainder for Seeds of Discovery, a CIMMYT-led project supported by Mexico’s Ministry of Agriculture, Livestock, Fisheries and Food (SAGARPA), which aims to unlock new wheat genetic diversity able to address climate change challenges.

Over the last two years, she has served as lead author for two published studies and co-author for four others. One used genotyping-by-sequencing loci and gene-based markers to examine the diversity of more than 1,400 spring bread wheat seed collections from key wheat environments. Another applied genome-wide association analysis on a selection of landrace collections from Turkey.

“In the first, we discovered not only thousands of new DNA marker variations in landraces adapted to drought and heat, but a new allele for the vernalization gene, which influences the timing of wheat flowering, and new alleles for genes controlling grain quality, all in landraces from near wheat’s center of origin in Asia and the Middle East.”

Sehgal acknowledges the as-yet limited impact of molecular markers in wheat breeding. “Individual markers generally have small effects on genetically complex traits like yield or drought tolerance; moreover, many studies fail to account for “epistasis,” the mutual influence genes have on one another, within a genome.”

To address this, she and colleagues have carried out the first study to identify genomic regions with stable expression for grain yield and yield stability, as well as accounting for their individual epistatic interactions, in a large sample of elite wheat lines under multiple environments via genome wide association mapping. A paper on this work has been accepted for publication in Nature Scientific Reports.

Sehgal has found her experience at CIMMYT enriching. “I feel free here to pursue the work I truly enjoy and that can make a difference, helping our center’s wheat breeders to create improved varieties with which farmers can feed a larger, more prosperous global population in the face of climate change and new, deadly crop diseases.”

Cutting-edge tools promote conservation, use of biodiversity

The CIMMYT maize germplasm bank holds 28,000 samples of unique maize genetic diversity that could hold the key to develop new varieties farmers need. Photo: Xochiquetzal Fonseca/CIMMYT.

EL BATAN, Mexico (CIMMYT) – Biodiversity is the building block of health for all species and ecosystems, and the foundation of our food system. A lack of genetic diversity within any given species can increase its susceptibility to stress factors such as diseases, pests, heat or drought for lack of the genetic variation to respond. This can lead to devastating consequences that include crop failures and extinction of species and plant varieties. Conserving and utilizing biodiversity is crucial to ensure the food security, health and livelihoods of future generations.

The 13th meeting of the Conference of the Parties (COP 13) to the Convention on Biological Diversity will be held in Cancún, Mexico, from December 5 to 17, 2016. Established in 1993 due to global concerns over threats to biodiversity and species extinctions, the Convention on Biological Diversity is an international, legally-binding treaty with three main objectives: the conservation of biological diversity; the sustainable use of the components of biological diversity; and the fair and equitable sharing of the benefits arising out of the utilization of genetic resources.

Mexico’s Secretariat of Agriculture (SAGARPA) has invited scientists from the International Maize and Wheat Improvement Center (CIMMYT) working with the MasAgro Biodiversidad (known in English as Seeds of Discovery, or SeeD) initiative to present at COP 13 on their work to facilitate the use of maize genetic diversity, particularly through a collection of tools and resources known as the “Maize Molecular Atlas.” The presentations will focus on how resources that have been developed can aid in the understanding of germplasm stored in genebanks and collections to enable better use.

As the region of origin and as a center of diversity for maize, Mexico and Mesoamerica are home to much of the crop’s genetic variation. Thousands of samples of maize from this and other important regions are preserved in the CIMMYT germplasm bank, in trust, for the benefit of humanity. The bank’s 28,000 maize seed samples hold diversity to develop new varieties for farmers to respond to challenges such as heat, disease and drought stress. However, information on the genetic makeup and physical traits of these varieties is often limited, making the identification of the most relevant samples difficult.

Native maize varieties, known as landraces, contain a broad amount of genetic diversity that could protect food security for future generations.
Native maize varieties, known as landraces, contain a broad amount of genetic diversity that could protect food security for future generations.

SeeD works to better characterize and utilize novel genetic diversity in germplasm banks to accelerate the development of new maize and wheat varieties for the benefit of farmers. The initiative has generated massive amounts of information on the genetic diversity of maize and wheat, as well as cutting-edge software tools to aid in its use and visualization. This information and tools are freely available as global public goods for breeders, researchers, germplasm bank managers, extension agents and others, but are even more powerful when they are integrated with different types and sets of data.

Developed by the SeeD initiative, the maize molecular atlas represents an unparalleled resource for those interested in maize genetic diversity.

“You can think of the maize molecular atlas like a satellite navigation system in your car,” said Sarah Hearne, a CIMMYT scientist who leads the project’s maize component. “Information that used to be housed separately, such as maps, traffic or the locations of police officers, gas stations, restaurants and hotels, are now brought together. It’s the same with the atlas. Having access to all of these data at once in an interlinked manner allows people to make better decisions, faster,” she said.

SeeD’s maize molecular atlas includes three main types of resources: data, such as maize landrace passport data (where it came from, when it was collected, etc.), geographic information system (GIS) -derived data (what the environment was like where maize was collected; rainfall, soils, etc.), genotypic data (genetic fingerprints of maize varieties) and available phenotypic data (information on how plants grow in different conditions); knowledge, (derived from data-marker trait associations; what bits of the genome do what); and tools, including data collection software (KDSmart), data storage and query tools (Germinate) and visualization tools (CurlyWhirly).

All of these resources are available through the SeeD website, where, when used together, they can increase the effective and efficient identification and utilization of maize genetic resources.

Interestingly, one of the first benefits of this initiative was for Mexican farmers. The efforts to better characterize the collection led to the identification of landraces that were resistant to Tar Spot, a disease that is devastating many farmers’ fields in Mexico and Central America. These landraces were immediately shared with farming communities while also being utilized in breeding programs. Smallholders in particular grow crops in diverse environmental conditions. They need diverse varieties. The understanding and use of biodiversity by researchers, breeders and farmers will be crucial to ensure the use of more and genetically diverse crops.

“With the atlas we now have the ability, with fewer resources, to interlink and query across different data types in one searchable resource,” Hearne said. This will allow breeders and researchers world-wide to hone in on the genetic and physical plant traits they are looking for, to more quickly identify and use novel genetic diversity to create improved varieties adapted to their specific needs. So far about 250 researchers and students from Mexico have participated in workshops and activities to begin using the new tools. With Mexico being a very important center of diversity for many species, agricultural and beyond, the same tools could be used for other species, here and abroad.

Hearne is looking forward to sharing information about MasAgro Biodiversidad and CIMMYT’s progress at COP 13, and is hopeful about the impacts the maize molecular atlas will have on biodiversity conservation.

“Conservation isn’t just preservation, it’s use. The molecular maize atlas enables us to better utilize the genetic resources we have, but also to better understand what diversity we may still need for our collection,” she said. “If you don’t know what you have, you don’t know what you need to preserve or look for. The work of the maize molecular atlas helps to address the underlying causes of biodiversity loss by raising awareness of the importance of these resources for sustainable food production while enabling researchers world-wide to use the information for assessing their own collections and generate more diverse varieties.”

SeeD is a multi-project initiative comprising: MasAgro Biodiversidad, a joint initiative of CIMMYT and the Mexican Ministry of Agriculture (SAGARPA) through the MasAgro (Sustainable Modernization of Traditional Agriculture) project; the CGIAR Research Programs on Maize (MAIZE CRP) and Wheat (WHEAT CRP); and a computation infrastructure and data analysis project supported by the UK’s Biotechnology and Biological Sciences Research Council (BBSRC). Learn more about the Seeds of Discovery project here

Sustainable agriculture poised to save Mayan rainforests from deforestation

Tour of field trials sown with MasAgro maize materials in Hopelchen, Campeche, Mexico. (Photo: CIMMYT)
Tour of field trials sown with MasAgro maize materials in Hopelchen, Campeche, Mexico. (Photo: CIMMYT)

EL BATAN, Mexico (CIMMYT) – Sustainable farming practices allow smallholder farmers to improve maize yields without increasing land, which has proven to reduce deforestation in Mexico’s Yucatan Peninsula according to an independent report commissioned by the Mexico REDD+ Alliance and The Nature Conservancy (TNC).

Conservation agriculture, a sustainable intensification technique that includes minimal soil movement, surface cover of crop residues and crop rotations, was successfully trialed in the south east of Mexico to protect biodiversity and counter rainforest loss caused by a creeping agricultural frontier, as part of a rural development project the Sustainable Modernization of Traditional Agriculture (MasAgro).

Over a year ago, the MasAgro project, led by the International Maize and Wheat Improvement Center (CIMMYT) and Mexico’s Secretariat of Agriculture (SAGARPA), partnered with local organization Pronatura Peninsula de Yucatan to test a sustainable intensification strategy in Hopelchen, a small community in the state of Campeche, where indigenous and Mennonite farmers grow maize following traditional farming practices.

Technician Vladimir May Tzun visits Santa Enna research platform to make fertility checks in Hopelchen, Campeche. (Photo: CIMMYT)
Technician Vladimir May Tzun visits Santa Enna research platform to make fertility checks in Hopelchen, Campeche. (Photo: CIMMYT)

Decades of plowing the fields without crop rotation and applying agrochemicals to control pests have degraded the soils in Hopelchen. As a result, farmers are prone to convert rainforest areas into growing fields to address diminishing crop yields. In an effort to curb this practice, MasAgro introduced conservation agriculture to improve soil fertility and water availability on the fields of five participant farmers.

A key moment during the project was when producers saw the benefits of conservation agriculture after two months of drought. Participant farmers achieved more developed maize cobs than those who did not, according to findings in the MasAgro case study featured in the report, “Experiences on sustainable rural development and biodiversity conservation in the Yucatan Peninsula.”

The positive results have sparked the interest of farmers from adjacent communities who want to get involved in the MasAgro project, said Pronatura’s field manager of sustainable agriculture, Carlos Cecilio Zi Dzib.

Maize growing in Santa Enna demonstration module in Hopelchen, Campeche, Mexico.
Maize growing in Santa Enna demonstration module in Hopelchen, Campeche, Mexico.

“MasAgro has been very successful in the Peninsula,” said Bram Govaerts, CIMMYT’s regional representative in Latin America. “In the course of its second year of implementation, MasAgro has established a research platform and offered training to 150 farmers, who have attended events organized in collaboration with TNC and Mexico’s Agriculture, Forestry and Livestock Research Institute.”

“This work is an effort to document the experiences of some of the sustainable rural initiatives and projects that contribute to reduce deforestation in the region, and thus make their contribution to the conservation and sustainable management of the Mayan Forest in the Yucatan Peninsula,” wrote report authors Carolina Cepeda and Ariel Amoroso.

SAGARPA and CIMMYT plan to present achievements of their MasAgro partnership, including the Hopelchen farmers’ success story, during the United Nations’ thirteenth meeting of the Conference of the Parties to the Convention on Biological Diversity (COP 13), which will take place from December 4 to 17 in Cancun, Mexico.

New online learning platform offers capacity development for all

Trainees work with KDSmart phenotyping technology, one of the subjects taught in the new SeeD distance learning modules. Photo: G. Salinas/CIMMYT
Trainees work with KDSmart phenotyping technology, one of the subjects taught in the new SeeD distance learning modules. Photo: G. Salinas/CIMMYT

EL BATAN, Mexico (CIMMYT) — An online learning platform created in partnership with the Seeds of Discovery (SeeD) initiative will revolutionize the project’s capacity development efforts, allowing SeeD to reach more users than ever before.

Distance learning modules consisting of practical and theory modules about how to enhance the use of genetic diversity in wheat and maize, will allow anyone in the world to benefit from SeeD’s collection of knowledge and tools regardless of location or income. These new distance learning modules are free and will be available online to the public in the future.

SeeD works to unlock and utilize novel genetic diversity held in genebanks to accelerate the development of improved maize and wheat varieties.  The initiative has generated massive amounts of invaluable information on the genetic diversity of maize and wheat, as well as cutting edge software tools to aid in its use and visualization.

“This information and tools have been made publicly available so that breeders and researchers around the world can develop improved crop varieties,” said Gilberto Salinas, head of capacity development at the SeeD initiative. “However, if people don’t know how to effectively utilize these datasets and software, the information is useless,” he said.

SeeD offers workshops on genetic diversity analysis, pre-breeding, and software tools will be offered free of charge several times a year, but space is limited, meaning that only a few researchers can be trained on SeeD’s data and technology each year.

“These modules will ensure that anyone can access and learn to effectively utilize our products, thus enabling the next generation of breeders and agricultural researchers in the tools that they will need to improve food security around the world,” Salinas said.

SeeD and CIMMYT’s first distance-learning module, which is hosted on the Moodle online learning platform, was developed by Laura Bouvet, a Ph.D. candidate in the department of plant science at Britain’s University of Cambridge, working with the National Institute of Agricultural Botany (NIAB). Bouvet, who participated in a three-month internship with SeeD said she is very excited about the number of people the modules will reach.

“So much information has been generated through the Seeds of Discovery project in terms of data and tools, and it’s very important that people can access and utilize this information for the greater good,” she said.  “These modules will complement SeeD workshops and will allow for higher impact of everything that has been generated through SeeD.”

KDSmart, one of the subjects taught in the new SeeD distance learning modules.
KDSmart, one of the subjects taught in the new SeeD distance learning modules.

The first module focuses on theory, introducing genotypic data, its importance for genetic diversity, how it is used, as well as the technologies that are used to generate and analyze the data.

The second module focuses on practice, guiding users through the process of using KDSmart, an Android based application to record phenotypic data, information on the physical traits of maize and wheat varieties. This module is being developed with the participation of several researchers from SeeD and the Genetic Resources Program led by Gilberto Salinas.

The modules also include two videos created by Bouvet in partnership with SeeD and CIMMYT, one to explain the Seeds of Discovery project, and another to introduce the platform to show how the modules can help prospective users solve problems they may face in their research.

The modules are directed at postgraduate students, crop breeders, university faculty members, and researchers. Currently, the modules and videos are available only in Spanish language, but English versions will be developed in the near future to reach even more people interested in genetic diversity.

“These distance learning modules are for everyone who wants to learn about genetic diversity, which is crucial to increase crop yields and is one of several important solutions to tackle climate change,” Bouvet said. “With distance learning modules, SeeD will be able to reach many more people, so that those without the time or financial means to physically come to CIMMYT can still benefit from their workshops and learn to utilize genetic diversity.”

SeeD is a multi-project initiative comprising: MasAgro Biodiversidad, a joint initiative of CIMMYT and the Mexican Ministry of Agriculture (SAGARPA) through the MasAgro (Sustainable Modernization of Traditional Agriculture) project; the CGIAR Research Programs on Maize (MAIZE CRP) and Wheat (WHEAT CRP); and a computation infrastructure and data analysis project supported by the UK’s Biotechnology and Biological Sciences Research Council (BBSRC). Learn more about the Seeds of Discovery project here

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Celebrating 50 years of collaboration: CIMMYT in the Mexican senate

eventosenadoMexico City, Mexico (CIMMYT) — In recognition of the contributions the International Maize and Wheat Improvement Center has made to agriculture and food security in Mexico and the world, a symposium was held 13th September at the Mexican Senate. Organized by the senate’s commission on agriculture and livestock, the symposium “50 years of CIMMYT in the Senate,” marked five decades of invaluable collaboration between the Center and the Mexican government.

CIMMYT was founded in Mexico in 1966 with the aim of improving food and nutritional security around the world. In the past 50 years, the agricultural research breakthroughs made by the Center and its scientists have produced nutritious and stress tolerant maize and wheat that has improved the lives and livelihoods of smallholder farmers and consumers around the world. None of these achievements would have been possible without the support of the Mexican Government, beginning with former president Adolfo Lopez Mateos’ support of the fledgling Center upon its founding and extending into the present day with cutting edge projects working to bring novel solutions to the challenges faced by Mexican agriculture.

“We are here today celebrating our 50th anniversary thanks to the leadership, vision and support of the Mexican people and their government that have allowed us to make this beautiful country our home,” said Martin Kropff, Director General of CIMMYT.

CIMMYT’s work has had incredible impact on the society and economies of Mexico and the world. “Around 50 percent of modern maize and wheat varieties planted around the world are descended from lines developed by CIMMYT,” Kropff announced. “Each year, these varieties generate between three and four billion dollars in profits for the farmers that grow them around the world.”

Kropff also recognized the important role that the Mexican government and other CIMMYT partners and allies, especially Mexico’s National Forestry, Agricultural and Livestock Research Institute (INIFAP), have played in this success. Fernando Flores Lui, Director General of INIFAP, referred to CIMMYT as a “pillar in the development of improved maize and wheat for the world,” and that their collaboration with the Center constitutes a “new model of collaboration based on equity and mutual support.”

simposio-senado-1In his welcome address to the symposium, Senator Manuel Cota Jiménez, president of the Mexican senate’s commission on agriculture and livestock, recognized the longstanding collaboration between CIMMYT and the Mexican government. “CIMMYT 50 marks a year for celebration, but also a year of challenges. CIMMYT has long worked to overcome the greatest challenges of agriculture in Mexico, it is our duty to continue working to ensure that our laws and public policies are in line with our goals for Mexican farmers and agriculture,” he said. “The countryside cannot remain isolated from science.”

One of the most fruitful examples of the success of this collaboration and partnership between CIMMYT and the Mexican government is the Sustainable Modernization of the Traditional Agriculture (MasAgro) project. A joint initiative of CIMMYT and Mexico’s Secretary of Agriculture, Livestock, Rural Development, Fisheries and Food (SAGARPA), MasAgro has developed 11 improved wheat varieties and over 40 improved maize varieties for the benefit of Mexican farmers.

In his keynote address, Bram Govaerts, director of MasAgro and CIMMYT’s regional representative in Latin America, presented on the project’s achievements over the past six years. A study from the University of Chapingo has shown that technicians who participate in the MasAgro project are nine times more efficient in their technical support to farmers than technicians who do not participate in the project. For the same amount of money invested, technicians using the MasAgro model reach nine times more farmers.

Govaerts also emphasized the importance of focusing on and supporting farmers in all components of agriculture to achieve the best results. “Planting improved seeds without agronomy is like trying to drive a racecar down a dirt road,” he said.

simposio-senado-2This support of the Mexican government will be equally crucial in the next 50 years if CIMMYT is to continue in its mission of improving food security and farmer livelihoods across the world. The relationship of collaboration and partnership between CIMMYT and the Mexican senate was strengthened and renewed through the open dialogue of the symposium, paving the way to implement the solutions necessary to ensure that improved maize and wheat varieties will be available not only to improve the lives and livelihoods of Mexican farmers, but to protect smallholder farmers and food security around the world.

“We still have so much left to accomplish, and that is why it is so important that we can count on the support of the legislators present today to maintain the budget for agricultural research and development,” Kropff said in his keynote address. “That is the objective of this symposium—to find solutions to the problems we face today and overcome them to achieve a food secure future for the Mexican people.”

The symposium was followed by a round table on maize and wheat improvement, agronomy and extension services held at the historic Casona de Xicoténcatl, the former headquarters of the Mexican senate. Over 30 researchers from CIMMYT and INIFAP participated in the event, as well as various representatives from different sectors of maize and wheat value chains in Mexico.

CIMMYT was honored by the attendance of Sanjaya Rajaram, 2014 World Food Prize laureate and keynote speaker at the symposium, and other distinguished guests including members of the Mexican senate’s commission on agriculture and livestock, senators María Hilaria Domínguez Arvizu, Silvia Garza Galván, Salvador López Brito and Adolfo Romero Lainas, as well as Patricia Ornelas Ruiz, director of the agrifood and fisheries information service (SIAP). The rectors of several Mexican agricultural universities also participated in the symposium, including Jesús Moncada de la Fuente of the Colegio de Posgraduados, Sergio Barrales Domínguez of the University of Chapingo and Jesús Valenzuela García of the Universidad Autónoma Agraria Antonio Narro. Representatives of various farmer groups and Mexican seed companies were also in attendance.

Syngenta-CIMMYT collaboration on helping smallholders stay safe

Javier Valdés is country head at Syngenta Mexico, a global seeds and crop protection company. Any opinions expressed are his own.

Improving productivity, fighting rural poverty and protecting the environment are among the significant challenges the Mexican agricultural sector faces. For Syngenta and the International Maize and Wheat Improvement Center (CIMMYT), responding to such demands is a priority and a key component of collaboration projects for promoting sustainable agricultural practices. That is why we have worked together since 2010.

More recently, under an agreement signed in 2013, we strengthened our joint commitment to meet the challenges that Mexican farmers are facing. This public-private collaboration is forged on CIMMYT’s aim to work with various sectors throughout society to establish strategic alliances and on the “Good Growth Plan” an initiative by which Syngenta has made six ambitious commitments with farmers and the environment to contribute to the global fight for food security.

One of these objectives has to do with Syngenta’s commitment to train 20 million smallholder farmers worldwide in the proper use and management of crop protection products, which play a key role in ensuring food security.

In Mexico, CIMMYT-trained technicians working on MasAgro (a research and capacity building project for sustainable intensification of maize and wheat systems funded by Mexico’s Agriculture Department, SAGARPA) are receiving specialized advice from Syngenta experts on the correct use and management of agrochemicals or BUMA, its acronym in Spanish.

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To date, Syngenta has offered the BUMA training to 130 technicians of the States of Mexico, Sonora and Guanajuato, who have, in turn, offered advice to groups of about 25 small farmers each. Moreover, CIMMYT’s knowledge-sharing methodology has a multiplier effect on the transfer of knowledge that increases the number of small farmers trained exponentially.

The BUMA training focuses on five key rules of pesticide application: understand products labeling; follow the labeling; regularly maintain equipment used for pesticide application; proper use of protective equipment and safe clean up practices. Furthermore, the training includes additional basic information about what to do in an emergency, and general information on first aid, among other topics.

Crop protection is vital for modern-day farming because it can substitute soil nutrients absent or depleted in poor soils and eradicate pests or control diseases that affect yields. While large scale farmers in developed countries often have access to crop protection products, smallholder farmers in developing countries face the challenges of applying optimal doses of fertilizer or pesticides to make products affordable but also to prevent environmental damage and increase yields.

The overall intention of the Syngenta-CIMMYT collaboration in Mexico is to improve the working conditions of smallholder producers who make up the majority of farmers, provide security for their families, highlight the importance of the role of crop protection and encourage them to continue using them sustainably.

Syngenta Mexico is a Gold Sponsor of CIMMYT’s 50th anniversary celebration in Mexico from 27-29 September 2016.

Ancient maize varieties provide modern solution to tar spot complex

Felix Corzo Jimenez , a farmer in Chiapas, Mexico, examines one of his maize plants infected with tar spot complex.
Felix Corzo Jimenez , a farmer in Chiapas, Mexico, examines one of his maize plants infected with tar spot complex. Photo: J. Johnson/CIMMYT.

CHIAPAS, Mexico — In southern Mexico and Central America a fungal maize disease known as tar spot complex (TSC) is decimating yields, threatening local food security and livelihoods. In El Portillo, Chiapas, Mexico, local farmer Felix Corzo Jimenez sadly surveys his maize field.

“It’s been a terrible year. We’ll be lucky if we harvest even 50 percent of our usual yields,” he said, examining a dried up maize leaf covered in tiny black dots, and pulling the husk off of an ear to show the shriveled kernels, poorly filled-in. “Tar spot is ruining our crops.”

Named for the black spots that cover infected plants, TSC causes leaves to die prematurely, weakening the plant and preventing the ears from developing fully, cutting yields by up to 50 percent or more in extreme cases. Caused by a combination of three fungal infections, the disease occurs most often in cool and humid areas across southern Mexico, Central America and South America. The disease is beginning to spread – possibly due to climate change, evolving pathogens and susceptible maize varieties – and was reported in important maize producing regions of central Mexico and the northern United States for the first time last fall. To develop TSC resistant maize varieties that farmers need, the Seeds of Discovery (SeeD) initiative is working to “mine” the International Maize and Wheat Improvement Center’s (CIMMYT) genebank for native maize varieties that may hold genes for resistance against the disease.

The first stage of fungal maize disease TSC, with tiny, black “tar spots” covering the leaf. The spots will soon turn into lesions that kill the leaf, preventing photosynthesis from occurring.
The first stage of fungal maize disease TSC, with tiny, black “tar spots” covering the leaf. The spots will soon turn into lesions that kill the leaf, preventing photosynthesis from occurring. Photo: J. Johnson/CIMMYT.

The majority of maize varieties planted in Mexico today are susceptible to TSC, meaning that farmers would have to spray expensive fungicides several times each year to protect their crops against the disease, a huge financial burden that few can afford. Creating varieties with natural resistance to tar spot is an economical and environmentally friendly option that will protect the livelihoods of the region’s smallholder maize farmers.

“This project targets the many farmers in the region with limited resources, and the small local seed companies that sell to farmers at affordable prices,” says Terry Molnar, SeeD maize breeder.

The key to developing maize varieties with resistance to TSC lies in the genetic diversity of the crop. For thousands of years, farmers have planted local maize varieties known as landraces, or descendants from ancient maize varieties that have adapted to their environment. Over centuries of selection by farmers these landraces accumulated specific forms of genes, or alleles, which helped them to resist local stresses such as drought, heat, pests or disease.

These novel genetic traits found in landrace maize can help breeders develop improved maize varieties with resistance to devastating diseases such as TSC. However, it is quite challenging for breeders to incorporate “exotic” landrace materials into breeding programs, as despite their resistance to stresses found in their native environment, they often carry unfavorable alleles for other important traits.

A maize ear with shriveled kernels that are poorly filled, a major side effect of TSC that reduces farmer’s tields.
A maize ear with shriveled kernels that are poorly filled, a major side effect of TSC that reduces farmer’s yields. Photo: J. Johnson/CIMMYT.

To help breeders incorporate this valuable genetic diversity into breeding programs, SeeD works to develop “bridging germplasm” maize varieties, which are created by transferring useful genetic variation from landraces held in the CIMMYT genebank into plant types or lines that breeders can readily use to develop the improved varieties farmers need. These varieties are created by crossing landrace materials with CIMMYT elite lines, and selecting the progeny with the genetic resistance found in a landrace without unfavorable traits breeders, farmers and consumers do not want.

“The CIMMYT maize genebank has over 28,000 maize samples from 88 countries, many of which are landraces that may have favorable alleles for disease resistance,” Molnar says. “We all know that there is good material in the bank, but it’s scarcely being used. We want to demonstrate that there are valuable alleles in the bank that can have great impact in farmers’ fields.”

A susceptible maize variety infected with TSC (left) compared to a healthy maize plant , a resistant variety immune to the disease (right).
A susceptible maize variety infected with TSC (left) compared to a healthy maize plant , a resistant variety immune to the disease (right). Photo: J. Johnson/CIMMYT.

SeeD scientists began by identifying landrace varieties with genetic resistance to TSC. Trials conducted in 2011, 2012 and 2014 evaluated a “core set” – a genetically diverse subset of the maize germplasm bank – in search of resistant varieties.  Of the 918 landrace varieties planted in 2011 and 2012, only two landraces—Oaxaca 280 and Guatemala 153—were outstanding for tolerance to the disease.  Genotypic data would later confirm the presence of unique resistant alleles not currently present in maize breeding programs that could be deployed into SeeD’s bridging germplasm. This bridging germplasm will be available to breeders for use in developing elite lines and varieties for farmers.

“As a breeder, I’m excited to work with SeeD’s bridging germplasm as soon as it is available,” said Felix San Vicente, CIMMYT maize breeder working with the CGIAR Research Program on Maize and the Sustainable Modernization of Traditional Agriculture (MasAgro) project.

Terry Molnar, maize breeder with SeeD, and Enrique Rodriguez, field research technician with SeeD, evaluate bridging germplasm for resistance to TSC.
Terry Molnar, maize breeder with SeeD, and Enrique Rodriguez, field research technician with SeeD, evaluate bridging germplasm for resistance to TSC. Photo: J. Johnson/CIMMYT.

Up to this point, most breeders have only used elite lines to develop hybrids, because landraces are extremely difficult to use. This practice, however, greatly limits the genetic diversity breeders employ. Using novel alleles from maize landraces allows breeders to develop improved hybrids while broadening the genetic variation of their elite germplasm. This novel genetic diversity is very important to protect crops from evolving pathogens, as it means the varieties will have several resistant alleles, including alleles that have never been used in commercial germplasm before.

“The more alleles the better,” said San Vicente, “as it protects the line longer. It provides a form of insurance to smallholder farmers as these varieties will have more genes for resistance, which reduces their risk of losing their crop.”

To ensure that farmers can access this improved seed, CIMMYT works with small local seed companies. “The price of seed will be very affordable,” according to San Vicente. “As CIMMYT is a non-profit, we provide our improved materials to seed companies at no cost.”

The TSC resistant bridging germplasm developed by SeeD has been tested in on-farm trials in TSC-prone sites in Chiapas and Guatemala, with promising results, and will be publicly available to breeders in 2017. In the meantime, local farmers look forward to seeing the results of this research in their own fields. “A variety with the disease resistance of a landrace and the yield and performance of a hybrid is exactly what we need,” says Corzo Jimenez.

Corzo Jimenez in his maize field infected with TSC. Varieties made from SeeD bridging germplasm would allow him to protect his crop without applying expensive fungicides.
Corzo Jimenez in his maize field infected with TSC. Varieties made from SeeD bridging germplasm would allow him to protect his crop without applying expensive fungicides. CIMMYT/Jennifer Johnson.

SeeD is a multi-project initiative comprising: MasAgro Biodiversidad, a joint initiative of CIMMYT and the Mexican Ministry of Agriculture (SAGARPA) through the MasAgro (Sustainable Modernization of Traditional Agriculture) project; the CGIAR Research Programs on Maize (MAIZE CRP) and Wheat (WHEAT CRP); and a computation infrastructure and data analysis project supported by the UK’s Biotechnology and Biological Sciences Research Council (BBSRC). To learn more about the Seeds of Discovery project, please go to http://seedsofdiscovery.org/.

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CIMMYT, Mexico honor legacy of Norman Borlaug

Norman Borlaug (fourth right) in the field showing a plot of Sonora-64, one of the semi-dwarf, high-yield, disease-resistant varieties that was key to the Green Revolution, to a group of young international trainees, at what is now CIMMYT's CENEB station (Campo Experimental Norman E. Borlaug, or The Norman E. Borlaug Experiment Station), near Ciudad Obregón, Sonora, northern Mexico. Photo: CIMMYT.
Norman Borlaug (fourth right) in the field showing a plot of Sonora-64, one of the semi-dwarf, high-yield, disease-resistant varieties that was key to the Green Revolution, to a group of young international trainees near Ciudad Obregón, Sonora, northern Mexico. Photo: CIMMYT.

To mark the 50th anniversary of the International Maize and Wheat Improvement Center (CIMMYT), Mexico’s Secretariat of Agriculture (SAGARPA) is displaying an exhibition honoring the life and legacy of Nobel Prize Laureate and CIMMYT scientist Norman Borlaug.

The exhibit, which opens from 25-27 May, includes photographs, personal items and awards that belonged to Borlaug and other CIMMYT scientists who made great strides in the center’s fight against hunger.

In his speech at the inauguration of the exhibit, CIMMYT Director General Martin Kropff emphasized the strong ties between Borlaug, CIMMYT and Mexico. “The work that Borlaug did in wheat with the support of Mexican farmers and scientists saved a billion lives around the world,” he said, and thanked SAGARPA for honoring Borlaug’s legacy with the event.  “Today, thanks to Borlaug, CIMMYT continues its work in Mexico to fight hunger around the world.”

A key part of this work is the MasAgro (Sustainable Modernization of Traditional Agriculture) project, a joint initiative between CIMMYT and SAGARPA that works to strengthen food security in Mexico. During his address, Kropff announced that the project has just developed 11 new varieties of wheat for Mexico, with genetic characteristics for high yield, pest resistance, and tolerance to climate change related stresses.

These wheat varieties are the result of 8 years of research and are the latest generation in a long line of cultivars generated from CIMMYT’s wheat breeding programs, dating from Borlaug himself to the present day. A recent wheat impact study found that 50 percent of the land used to grow wheat around the world is planted with CIMMYT or CIMMYT-derived varieties, feeding billions across the globe.

CIMMYT Director General Martin Kropff and Mexico’s Secretary of Agriculture, José Eduardo Calzada Rovirosa display the signed agreement. Photo: CIMMYT.
CIMMYT Director General Martin Kropff and Mexico’s Secretary of Agriculture, José Eduardo Calzada Rovirosa display the signed agreement. Photo: CIMMYT.

In his welcome address, Mexico’s Secretary of Agriculture, José Eduardo Calzada Rovirosa praised Borlaug and CIMMYT’s work, and emphasized the importance of protecting food security both in Mexico and around the world. “The topic of food security is becoming more and more important every day. According to the FAO, food production must increase by 70% by 2050 in order to keep up with demand,” he said.

Calzada Rovirosa and Kropff signed an agreement between CIMMYT and SAGARPA to continue supporting MasAgro’s work and its contribution to Mexico’s food security.

“We are very proud here at CIMMYT to have the support of SAGARPA and Mexico’s Agriculture Secretary for our work,” Kropff said. “We are the only international organization based in Mexico, and truly have such a strong relationship with our host country.”

Julie Borlaug (center) presents Calzada Rovirosa (right) and Kropff (left) her grandfather's Order of the Aztec Eagle award.
Julie Borlaug (center) presents her grandfather’s Order of the Aztec Eagle award to Calzada Rovirosa (right) and Kropff (left).

At the close of the inauguration, Julie Borlaug, granddaughter of Norman Borlaug and associate director for external relations at the Norman Borlaug Institute for International Agriculture at Texas A&M University, presented the Secretary of Agriculture with her grandfather’s “Order of the Aztec Eagle” medal. The Aztec Eagle is the highest honor the government of Mexico awards to foreign citizens, and previous winners include Queen Elizabeth II and Nelson Mandela.  Norman Borlaug received the medal in 1970 upon winning the first Nobel Peace Prize awarded for agriculture, putting CIMMYT and Mexico’s work to protect global food security in the international spotlight. The medal will be displayed at SAGARPA as part of the Borlaug exhibition. “The order of the Aztec Eagle was one of my grandfather’s greatest honors, and our family is happy to see it displayed here in Mexico for the first time,” she said. “We know that CIMMYT in Mexico will produce the next Norman—or Norma—Borlaug that will help feed the world. Thank you SAGARPA for your continued support.”

The event was also attended by the sub secretary of Agriculture, Jorge Narváez Narváez; sub secretary of rural development, Mely Romero Celis; attorney general of SAGARPA, Mireille Rocatti Velázquez; international affairs coordinator, Raúl Urteaga Trani; director general of Mexico’s Development Fund for Agriculture, Livestock, Forests and Fisheries, Juan Carlos Cortés García; as well as ambassadors and representatives of Australia, Georgia, Pakistan and Malaysia.

MasAgro named a project transforming Mexico by leading university

MasAgro named a project transforming Mexico. Photographer: MasAgro/CIMMYT
MasAgro named a project transforming Mexico. Photographer: MasAgro/CIMMYT


MONTERREY, Mexico – The Sustainable Modernization of Traditional Agriculture (MasAgro) project of Mexico’s Secretariat of Agriculture, Livestock, Rural Development, Fisheries and Food, in close collaboration with the International Maize and Wheat Improvement Center (CIMMYT), was recognized by Universidad Tecnológico y Estudios Superiores de Monterrey (ITESM) as being one of “10 projects that are transforming Mexico.”

“This project is transforming Mexico and, four years after its launch, it has benefited more than 200,000 farmers,” said Silverio García Lara, professor at ITESM’s School of Engineering and Science, Monterrey campus. “The project focuses on the base of the productive and economic pyramid,” explained the researcher, who favors “developing cutting-edge technologies to regenerate and renew Mexican farming.”

ITESM is involved in a project focusing on biotechnology for food security that applies cutting-edge technologies to analyze MasAgro’s new maize varieties and ensure they meet the nutrition and processing quality parameters of the Mexican market, explained Natalia Palacios, the person responsible for CIMMYT’s Maize Quality Laboratory, in an information bulletin that was broadcast when the winning project was presented at the 46th Research and Development Congress held on 20-22 January at ITESM’s Student Center, Monterrey campus.

“We are very proud of MasAgro because its results in the lab and especially in farmers’ fields have been widely recognized both nationally and internationally; today ITESM, a research partner that has collaborated with us since the beginning, also recognizes the project,” said Bram Govaerts, Leader of CIMMYT’s Sustainable Intensification Strategy for Latin America.

Among MasAgro’s main achievements, Govaerts highlighted the adoption of sustainable intensification of basic grain production on half a million hectares. He also emphasized the development of 20 high yielding maize hybrids which, combined with MasAgro’s sustainable agronomic practices, have increased rainfed maize farmers’ income by 9-31%. According to Govaerts, 16 precision machines for use in different production situations, from subsistence to intensive or commercial farming, and different postharvest storage solutions are among the technologies that MasAgro offers.

“Our farmers out in the fields are very interested in innovating and obtaining new technologies coming from the labs and from international research institutions such as CIMMYT,” stated García Lara when presenting ITESM’s award for the work done by MasAgro.

Researchers race to rescue wheat sample in war-torn Syria

After wheat seeds are planted in the greenhouse, the samples are then harvested and prepared to be sent to the laboratory for DNA extraction and genotyping. Photo: Carolina Sansaloni/CIMMYT

EL BATAN, Mexico (CIMMYT) – With Syria torn apart by civil war, a team of scientists in Mexico and Morocco are rushing to save a vital sample of wheat’s ancient and massive genetic diversity, sealed in seed collections of an international research center formerly based in Aleppo but forced to leave during 2012-13.

The researchers are restoring and genetically characterizing more than 30,000 unique seed collections of wheat from the Syrian genebank of the International Center for Agricultural Research in the Dry Areas (ICARDA), which has relocated its headquarters to Beirut, Lebanon, and backed up its 150,000 collections of barley, fava bean, lentil and wheat seed with partners and in the Global Seed Vault at Svalbard, Norway.

In March 2015, scientists at ICARDA were awarded The Gregor Mendel Foundation Innovation Prize for their courage in securing and preserving their seed collections at Svalbard, by continuing work and keeping the genebank operational in Syria even amidst war.

“With war raging in Syria, this project is incredibly important,” said Carolina Sansaloni, genotyping and DNA sequencing specialist at the Mexico-based International Maize and Wheat Improvement Center (CIMMYT), which is leading work to analyze the samples and locate genes for breeding high-yield, climate resilient wheats. “It would be amazing if we could be just a small part of reintroducing varieties that have been lost in war-torn regions.”

Treasure from wheat’s cradle to feed the future

Much of wheat seed comes from the Fertile Crescent, a region whose early nations developed and depended on wheat as the vital grain of their civilizations. The collections could hold the key for future breeding to feed an expanding world population, according to Sansaloni.

“An ancient variety bred out over time could contain a gene for resistance to a deadly wheat disease or for tolerance to climate change effects like heat and drought, which are expected to become more severe in developing countries where smallholder farmers and their families depend on wheat,” she explained.

Cross-region partners, global benefits

Sansaloni’s team has been sequencing DNA from as many as 2,000 seed samples a week, as well as deriving molecular markers for breeder- and farmer-valued traits, such as disease resistance, drought or heat tolerance and qualities that contribute to higher yields and grain quality.

They are using a high-end DNA sequencing system located at the Genetic Analysis Service for Agriculture (SAGA), a partnership between CIMMYT and Mexico’s Secretariat of Agriculture, Livestock, Rural Development, Fisheries and Food (SAGARPA), and with the support of a private company from Australia, Diversity Arrays Technology.

The sequencer at SAGA can read 1600 samples of seed at once and develops more data than ever before. The HiSeq 2500 boils down data and shows the information at a “sequence level”, for example, height variations among wheat varieties.

Worldwide, there are few other machines that produce this kind of data and most are owned by private companies, explained Sansaloni. This was the first non-Latin American based project used by the HiSeq 2500.

“The success of this project shows what a fantastic opportunity for international collaboration we now have,” Sansaloni said. “I can’t even put a value on the importance of the data we have collected from this project. It’s priceless.”

After data has been collected, seed samples will be “regenerated” by ICARDA and CIMMYT. That is, the process of restoring old seed samples with healthy new seeds.

ICARDA and CIMMYT will share seed and data from the project and make these results available worldwide.

“With these new seeds, we hope to reconstruct ICARDA’s active and base collection of seeds over the next five years in new genebank facilities in Lebanon and Morocco,” said Fawzy Nawar, senior genebank documentation specialist, ICARDA.

Funded through the CGIAR Research Program on Wheat, the effort benefits both of the international centers, as well as wheat breeding programs worldwide, said Tom Payne, head of CIMMYT’s Wheat Germplasm Bank. “ICARDA is in a difficult situation, with a lack of easy access to their seeds and no facilities to perform genotyping,” he explained. “This was the perfect opportunity to collaborate.”

New book details benefits of holistic approach to agricultural development in Mexico

foto-nota-4The creation, development and strengthening of farmers’ and technicians’ capacities are a pillar of the MasAgro strategy. For this reason, besides investing in research and technological development, SAGARPA and CIMMYT are also investing in building the capacities of agents of change to foster testing and implementation of new technologies with farmers. To this end, as part of the MasAgro Productor (Take It to the Farmer) extension strategy, in 2013, they set up a network of trainers responsible for detecting areas of opportunity for the technicians they supervise and designing a training plan to develop their technical and social skills, which will enable them to innovate together with farmers.

Thanks to this information and their efforts, a book titled Options Available for Implementing MasAgro Technologies has been published which analyzes the options available for implementing sustainable agricultural practices and technologies in 23 Mexican entities where MasAgro instructors have supported, mentored, followed up and evaluated the agents of change who have provided technical backup to farmers involved in this strategy.

This systems focus of agricultural innovation fosters interaction among different actors with the purpose of developing new technologies, better practices, new processes and new ways of organizing and managing. This in turn promotes learning and sets aside the old innovation paradigm––which considers innovation to be the result solely of scientific research and technological development––and regards innovation as a collective event for which everyone is responsible.

This option includes the hubs’[i] physical infrastructure and technological tools, agronomic management solutions and the human capital that has developed within the framework of MasAgro’s extension activities. The book also talks about various constraints and the potential for implementing the technologies promoted by MasAgro in different states of Mexico. This information is organized into five regions based on the distribution of MasAgro trainers: North (Chihuahua, Durango, San Luis Potosí and Zacatecas), Center (Estado de México, Guerrero, Hidalgo and Morelos) and West (Aguascalientes, Colima, Guanajuato, Jalisco, Michoacán and Querétaro).

This document will undoubtedly be useful to farmers, technicians, researchers, decision makers, input suppliers, development organizations, traders and other actors involved in MasAgro’s innovation network.

Consult the book at: http://conservacion.cimmyt.org/index.php/es/component/docman/doc_view/1618-libro-de-oferta-tecnologica-masagro

 

[i] A hub is an agricultural innovation node and a model of agricultural extension where farmers interact with technicians and technicians interact with researchers. These innovation nodes usually have similar agroecological conditions for establishing production systems where different technologies promoted by the MasAgro program are developed, disseminated, adapted and improved (for more information, go to http://conservacion.cimmyt.org).

With information from MasAgro

Learning partnerships turn research into results for Mexican agriculture

“CIMMYT is famous for helping farmers all over the world, but what fewer people know is that they also help Mexican researchers and students who will become the next generation of researchers through the courses and workshops they offer,” said Alejandro Ledesma, maize researcher at Mexico’s National Forestry, Agricultural and Livestock Research Institute (INIFAP). Above, Ledesma (L), receives certificate from CIMMYT Director General Martin Kropff, Juan Burgueño Ferreira, Head of CIMMYT’s Biometrics and Statistics Unit, and Kevin Pixley, Head of the Genetic Resources Program at a course on statistical analysis of genetic and phenotypic data for breeders held at CIMMYT. Photo: CIMMYT

The Seeds of Discovery (SeeD) project seeks to empower the next generation of Mexican scientists to use maize and wheat biodiversity to effectively meet the needs of Mexican agriculture in the future. By providing professional agricultural research and development opportunities for current and future maize and wheat scientists, SeeD works to ensure that the materials they develop will reach those who need it most. For this reason, SeeD is developing a platform of publicly available data and software tools that enable the efficient use of maize and wheat genetic resources. These genetic resources, or biodiversity, include more than 28,000 maize and 140,000 wheat samples, known as accessions, that are conserved in CIMMYT’s seed bank and available to researchers worldwide.

Genetic resources are the raw materials or building blocks used to develop new maize and wheat varieties needed to meet the demands of a growing population in a changing climate. Many of these maize and wheat accessions contain positive traits such as drought tolerance or disease resistance, which if bred into new varieties  have the potential to improve food security and livelihoods in countries such as Mexico in the global south.

However, the specific potential impact of SeeD on Mexican agriculture and society will only be realized if breeders and scientists effectively use the products resulting from the project. By inviting researchers, professors and students to participate in workshops, training courses and diverse research projects, a growing cadre of scientists is learning how to use the databases and software tools developed by SeeD and validating their utility.

Cynthia Ortiz places DNA samples into a thermal cycler in the CIMMYT Biosciences laboratory. Photo: Jennifer Johnson

“Sharing the knowledge generated by SeeD and making it available to the scientific community will help accelerate the development of new varieties that will benefit long-term food security in Mexico and the world,” said Cynthia Ortiz, a graduate student in biotechnology at the Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV) in Mexico City.

Ortiz is conducting research for her Master of Science thesis mentored by SeeD scientist Sukhwinder Singh, who is helping her map the quantitative trait loci (QTL) for phenological and grain yield-related traits in wheat varieties created by crossing synthetic wheat varieties with elite lines. She has participated in two SeeD workshops focusing on wheat phenotyping for heat, drought and yield as well as on the use of the maize and wheat molecular atlas, where she learned to use SeeD software such as Flapjack and CurlyWhirly to visualize the results of genetic diversity analyses.

“The materials SeeD has developed have opened the door for identifying genetic resources with positive traits such as heat and drought tolerance, or resistance to pests and diseases that affect crops all over the world,” Ortiz said. “And the best part is that at the same time, they have sought to protect the genetic diversity of these crops, using the native biodiversity we have in Mexico and the world to confront the challenge of ensuring food security.”

David Gonzalez, a recent graduate of the Chapingo Autonomous University in Texcoco, a city about 30 km (20 miles) from Mexico City, agrees. He worked with SeeD scientists Sarah Hearne and Terence Molnar on his Master of Science thesis, identifying genetic resources with resistance to the maize leaf disease “tar spot complex” (TSC) by using genome-wide association study (GWAS) and genomic selection.

David Gonzalez (L) scores maize plants for signs of tar spot disease alongside SeeD scientist Terence Molnar (R) in the state of Chiapas, Mexico. Photo: Jennifer Johnson

“The software and databases SeeD develops for analyzing genotypic and phenotypic data are novel tools that can be used for research as well as academic purposes,” Gonzalez said. “They are a valuable resource that can be utilized by academic institutions to train students in genetic analysis.”

Gonzalez attended the CIMMYT training course “Technologies for Tropical Maize Improvement,” where he learned about new tools for field trial design, data analysis, doubled haploid technology, molecular markers, GWAS and genomic selection.

“This training, as well as the valuable help and support from CIMMYT scientists, really helped me develop myself professionally,” he said. “It was exciting to work with such an ambitious project, doing things that have never been done before to discover and utilize maize and wheat genetic diversity for the benefit of farmers. I look forward to using what I’ve learned in my future career to develop varieties that meet the needs of farmers in Latin America.”

SeeD is a joint initiative of CIMMYT and the Mexican Ministry of Agriculture (SAGARPA) through the MasAgro project. SeeD receives additional funding from the CGIAR Research Programs on Maize (MAIZE CRP) and Wheat (WHEAT CRP), and from the UK’s Biotechnology and Biological Sciences Research Council (BBSRC).

Learning partnerships turn research into results for Mexican agriculture

“CIMMYT is famous for helping farmers all over the world, but what fewer people know is that they also help Mexican researchers and students who will become the next generation of researchers through the courses and workshops they offer,” said Alejandro Ledesma, maize researcher at Mexico’s National Forestry, Agricultural and Livestock Research Institute (INIFAP). Above, Ledesma (L), receives certificate from CIMMYT Director General Martin Kropff, Juan Burgueño Ferreira, Head of CIMMYT’s Biometrics and Statistics Unit, and Kevin Pixley, Head of the Genetic Resources Program, at a course on statistical analysis of genetic and phenotypic data for breeders held at CIMMYT. Photo: CIMMYT
“CIMMYT is famous for helping farmers all over the world, but what fewer people know is that they also help Mexican researchers and students who will become the next generation of researchers through the courses and workshops they offer,” said Alejandro Ledesma, maize researcher at Mexico’s National Forestry, Agricultural and Livestock Research Institute (INIFAP). Above, Ledesma (L), receives certificate from CIMMYT Director General Martin Kropff, Juan Burgueño Ferreira, Head of CIMMYT’s Biometrics and Statistics Unit, and Kevin Pixley, Head of the Genetic Resources Program, at a course on statistical analysis of genetic and phenotypic data for breeders held at CIMMYT. Photo: CIMMYT

The Seeds of Discovery (SeeD) project seeks to empower the next generation of Mexican scientists to use maize and wheat biodiversity to effectively meet the needs of Mexican agriculture in the future. By providing professional agricultural research and development opportunities for current and future maize and wheat scientists, SeeD works to ensure that the materials they develop will reach those who need them most. For this reason, SeeD is developing a platform of publicly available data and software tools that enable the efficient use of maize and wheat genetic resources. These genetic resources, or biodiversity, include more than 28,000 maize and 140,000 wheat samples, known as accessions, that are conserved in CIMMYT’s seed bank and available to researchers worldwide.

Genetic resources are the raw materials or building blocks used to develop new maize and wheat varieties needed to meet the demands of a growing population in a changing climate. Many of these maize and wheat accessions contain positive traits such as drought tolerance or disease resistance, which, if bred into new varieties, have the potential to improve food security and livelihoods in countries such as Mexico in the global south.

However, the specific potential impact of SeeD on Mexican agriculture and society will only be realized if breeders and scientists effectively use the products resulting from the project. By inviting researchers, professors and students to participate in workshops, training courses and diverse research projects, a growing cadre of scientists is learning how to use the databases and software tools developed by SeeD and validating their utility.

Cynthia Ortiz places DNA samples into a thermal cycler in the CIMMYT Biosciences laboratory. Photo: CIMMYT/J. Johnson

“Sharing the knowledge generated by SeeD and making it available to the scientific community will help accelerate the development of new varieties that will benefit long-term food security in Mexico and the world,” said Cynthia Ortiz, a graduate student in biotechnology at the Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV) in Mexico City.

Ortiz is conducting research for her Master of Science thesis mentored by SeeD scientist Sukhwinder Singh, who is helping her map the quantitative trait loci (QTL) for phenological and grain yield-related traits in wheat varieties created by crossing synthetic wheat varieties with elite lines. She has participated in two SeeD workshops focusing on wheat phenotyping for heat, drought and yield as well as on the use of the maize and wheat molecular atlas, where she learned to use SeeD software such as Flapjack and CurlyWhirly to visualize the results of genetic diversity analyses.

“The materials SeeD has developed have opened the door for identifying genetic resources with positive traits such as heat and drought tolerance, or resistance to pests and diseases that affect crops all over the world,” Ortiz said. “And the best part is that at the same time, they have sought to protect the genetic diversity of these crops, using the native biodiversity we have in Mexico and the world to confront the challenge of ensuring food security.”

David Gonzalez, a recent graduate of the Chapingo Autonomous University in Texcoco, a city about 30 km (20 miles) from Mexico City, agrees. He worked with SeeD scientists Sarah Hearne and Terence Molnar on his Master of Science thesis research, identifying genetic resources with resistance to the maize leaf disease “tar spot complex” (TSC) by using genome-wide association study (GWAS) and genomic selection.

David Gonzalez (L) scores maize plants for signs of tar spot disease alongside SeeD scientist Terence Molnar (R) in the state of Chiapas, Mexico. Photo: CIMMYT/J. Johnson

“The software and databases SeeD develops for analyzing genotypic and phenotypic data are novel tools that can be used for research as well as academic purposes,” Gonzalez said. “They are a valuable resource that can be utilized by academic institutions to train students in genetic analysis.”

Gonzalez attended the CIMMYT training course “Technologies for Tropical Maize Improvement,” where he learned about new tools for field trial design, data analysis, doubled haploid technology, molecular markers, GWAS and genomic selection.

“This training, as well as the valuable help and support from CIMMYT scientists, really helped me develop myself professionally,” he said. “It was exciting to work with such an ambitious project, doing things that have never been done before to discover and utilize maize and wheat genetic diversity for the benefit of farmers. I look forward to using what I’ve learned in my future career to develop varieties that meet the needs of farmers in Latin America.”

SeeD is a joint initiative of CIMMYT and the Mexican Ministry of Agriculture (SAGARPA) through the MasAgro project. SeeD receives additional funding from the CGIAR Research Programs on Maize (MAIZE CRP) and Wheat (WHEAT CRP), and from the UK’s Biotechnology and Biological Sciences Research Council (BBSRC).

Mobilizing gene bank biodiversity in the fight against climate change

Ancestors of modern wheat (R) in comparison with an ear of modern cultivated wheat (L). Photo: Thomas Lumpkin/CIMMYT.

In a world where the population is expected to reach 9 billion by the year 2050, grain production must increase to meet rising demand. This is especially true for bread wheat, which provides one-fifth of the total calories consumed by the world’s population. However, climate change threatens to derail global food security, as instances of extreme weather events and high temperatures reduce agricultural productivity and are increasing faster than agriculture can naturally adapt, leaving our future ability to feed the global population uncertain. How can we ensure crop production and food security for generations to come?

In order to continue feeding the planet, it is imperative that we identify crop varieties that display adaptive and quality traits such as drought and heat stress tolerance that will allow them to survive and flourish despite environmental stresses. For this reason, a recent study by Sehgal et al., “Exploring and mobilizing the gene bank biodiversity for wheat improvement,” was conducted to characterize wheat seed samples in the CIMMYT germplasm bank to identify useful variations for use in wheat breeding.

The study analyzed the genetic diversity of 1,423 bread wheat seed samples that represent major wheat production environments around the world, particularly regions that experience significant heat and drought. The tested samples included synthetic wheat varieties, which are novel bread wheat varieties created by making crosses between the progenitors of modern bread wheat, durum wheat and wild grassy ancestors; landraces, which are local varieties developed through centuries of farmer selection; and elite lines that have been selectively bred and adapted. The samples were analyzed through genotyping-by-sequencing, a rapid and cost-effective approach that allows for an in-depth, reliable estimate of genetic diversity.

The results of the study suggested that many of the tested landraces and synthetics have untapped, useful genetic variation that could be used to improve modern wheat varieties. When combined with elite wheat germplasm, this genetic variation will increase stress adaptation and quality traits as well as heat and drought tolerance, thus leading to new wheat varieties that can better survive under climate change. The study also found new genetic variation for vernalization, in which flowering is induced by exposure to cold, and for glutenin, a major wheat protein responsible for dough strength and elasticity. Based on the information generated by the study, over 200 of the diverse seed samples tested have been selected for use in breeding, since they contain new specific forms of genes conferring drought and heat stress tolerance. This new genetic diversity will help bread wheat breeding programs around the world create new varieties to feed the world’s growing population in a changing environment.

This research is part of CIMMYT’s ongoing Seeds of Discovery (SeeD) project, which is funded by the Mexican Ministry of Agriculture, Livestock, Rural Development, Fisheries and Food (SAGARPA) through the Sustainable Modernization of Traditional Agriculture (MasAgro) project, as well as the CGIAR Research Program on Wheat (WHEAT). SeeD works to unlock the genetic potential of maize and wheat genetic resources by providing breeders with a toolkit that enables their more targeted use in the development of better varieties that address future challenges, including those from climate change and a growing population.

To read the full study, please click here:

Citation:

Sehgal D, Vikram P, Sansaloni CP, Ortiz C, Pierre CS, Payne T, et al. (2015) Exploring and Mobilizing the Gene Bank Biodiversity for Wheat Improvement. PLoS ONE 10(7): e0132112. doi:10.1371/journal.pone.0132112

Related Publications:

Exploiting genetic diversity from landraces in wheat breeding for adaptation to climate change (2015) Lopes, M.S., El-Basyoni, I., Baenziger, P.S., Sukhwinder-Singh, Royo, C., Ozbek, K., Aktas, H., Ozer, E., Ozdemir, F., Manickavelu, A., Ban, T., Vikram, P.

Coping with climate change: the roles of genetic resources for food and agriculture, Food and Agriculture Organization of the United Nations (FAO)

Wheat scientists eye USDA $3.4 million in new funds to boost yields

CIMMYT wheat physiologist Matthew Reynolds describes the technology used for conducting research into heat and drought resilient wheat varieties in Ciudad Obregon in Mexico's northern state of Sonora in March 2015. CIMMYT/Julie Mollins
CIMMYT wheat physiologist Matthew Reynolds describes the technology used for conducting research into heat and drought resilient wheat varieties in Ciudad Obregon in Mexico’s northern state of Sonora in March 2015. CIMMYT/Julie Mollins

EL BATAN, Mexico (CIMMYT) – Scientists involved in a major global initiative aimed at increasing wheat yields as much as 60 percent by 2050 got a recent boost when the U.S. government announced $3.4 million in new research funds.

Researchers affiliated with the International Wheat Yield Partnership (IWYP), focused on developing new high-yielding varieties of wheat to meet demand that will be generated by a projected 33 percent increase in population growth from 7.3 billion people today to 9.5 billion by 2050, will be eligible to apply for the grant money.

“This opens up new opportunities for scientists in the United States to provide invaluable input to the overall project of increasing yields, improving our potential to tackle this vital work to achieve global food security,” said Matthew Reynolds, wheat physiologist at the Mexico-based International Maize and Wheat Improvement Center (CIMMYT), one of the founding members of IWYP.

Awards for the new funds announced last Monday by Tom Vilsack, the U.S. agriculture secretary, will be made available through the U.S. Department of Agriculture National Institute of Food and Agriculture (NIFA) Agriculture and Food Research Initiative (AFRI).

“Wheat is one of the world’s most important staple crops, providing a significant amount of daily calories and protein throughout the world,” Vilsack said.

“By 2050, the demand for wheat as part of a reliable, affordable, and nutritious diet will grow alongside the world population, and continued wheat research will play an important role in ensuring its continued availability.”

IWYP, which targets partner investments of up to $100 million, supports the G20 Wheat Initiative in its efforts to enhance the genetic component of wheat yield and develop new wheat varieties adaptable to different geographical regions and environments.

CLIMATE RISKS

Wheat yields face threats from global warming. Findings in a report from the Intergovernmental Panel on Climate Change (IPCC) state that it is very likely that heat waves will occur more often and last longer throughout the 21st century and rainfall will be more unpredictable.

Mean surface temperatures could potentially rise by between 2 to 5 degrees Celsius or more, the report said.

“Wheat currently provides 20 percent of calories and protein consumed worldwide and current models show that a 2 degree increase in temperature would lead to 20 percent reduction in wheat yield and that a 6 degree increase would lead to a 60 percent reduction,” Reynolds said.

“If we have a 40 percent yield reduction due to climate change, the risks to food security will be increased because wheat production has to increase by 60 percent just to keep up with population projections.”

In addition to CIMMYT, IWYP members include Britain’s Biotechnology and Biological Sciences Research Council (BBSRC), Mexico’s Secretariat of Agriculture, Livestock, Rural Development, Fisheries and Food (SAGARPA), the U.S. Agency for International Development (USAID), the Grains Research and Development Corporation of Australia (GRDC), the Department of Biotechnology of India (DBT), Agriculture and Agri-Food Canada (AAFC), the Institut National de la Recherche Agronomique (INRA) in France and the Syngenta Foundation for Sustainable Agriculture (SFSA) in Switzerland.

Applications are due May 3, 2016 and more information is available via the NIFA-IWYP request for applications.