In the dynamic landscape of wheat breeding, early access to germplasm emerges as a strategic catalyst for accelerating variety turnover and meeting the evolving challenges faced by farmers in South Asia. Since its inception, the Accelerating Genetic Gains in Maize and Wheat (AGG) project has pioneered new tools to optimize the wheat breeding process. One such tool, the efficient and low-cost 3-year breeding cycle, has been fine-tuned in Mexico, using the Toluca screenhouse and field advancement in Obregón, laying the groundwork for faster variety turnover.
The inaugural set of lines generated through this enhanced breeding cycle is already undergoing Stage 1 trials in the Obregón 2023-24 season. However, the innovation doesn’t stop there; to expedite the variety release process and garner robust data from the Target Population of Environments (TPE), Stage 2 lines are being rigorously tested at over 20 sites in South Asia through collaboration with National Agricultural Research and Extension Services (NARES) partners. In the seasons spanning 2021-2024, a total of 918 Stage 2 lines underwent rigorous trials, aiming to provide early access to improved wheat lines for testing and release by NARES and establish a genetic correlation matrix between Obregón selection environments and diverse sites across South Asia.
These extensive trials serve a dual purpose. Firstly, they facilitate early access to improved wheat lines for testing and release by NARES, bolstering the agricultural landscape with resilient and high-yielding varieties. Secondly, they contribute to the establishment of a genetic correlation matrix between the selection environments in Obregón and the diverse sites across South Asia. This matrix becomes a guiding compass, aiding in selecting the most promising lines for broader TPEs in South Asia and beyond.
Transformative impact on wheat varieties in South Asia
Through the support of our partners and funders from the Bill & Melinda Gates Foundation, the Foundation for Food and Agriculture Research (FFAR), the UK Foreign, Commonwealth & Development Office (FCDO), and the US Agency for International Development (USAID), great achievements have been recorded throughout the region. India, a prominent player in wheat cultivation, stands as a testament to the transformative impact of early access to advanced lines. The top three varieties, namely DBW187, DBW303, and DBW 222, covering over 6 million hectares, trace their roots to CIMMYT varieties. Adopting a fast-track approach through early-stage testing of these advanced lines at BISA sites in India, supported by the Delivering Genetic Gain in Wheat (DGGW) project, facilitated the release of these varieties two years ahead of the regular testing process. This expedited varietal release was complemented by the innovative early seed multiplication and dissemination approach introduced by the Indian Council of Agricultural Research (ICAR). Recent additions to this accelerated channel include varieties such as DBW 327, DBW 332, DBW 370, and 371, promising further advancements in wheat cultivation.
Pakistan
In Pakistan, the early access to advanced lines has been a catalyst for releasing high-yielding, climate-resilient, and nutritious wheat varieties. In 2023 alone, 12 new varieties were released, with the renowned ‘Akbar-19,’ introduced in 2019, covering a substantial 42% of cultivated land in Punjab. Data released by the Ayub Agricultural Research Institute (AARI), shows that this variety, known for its high yield potential, disease resistance, and enriched zinc content, has significantly contributed to increased wheat production in the region.
Nepal
Guided by policy interventions in the national varietal testing process, Nepal has experienced the fast-track commercialization of high-yielding and climate-resilient wheat varieties. Allowing multilocation testing of CIMMYT nurseries and advanced elite lines, Nepal released six biofortified zinc wheat varieties in 2020. The expeditious seed multiplication of these released and pre-release varieties has facilitated the rapid spread of new and improved wheat varieties.
The strategic utilization of early access to wheat germplasm in South Asia holds promise in accelerating variety turnover, offering farmers resilient and high-performing wheat varieties. Collaborative efforts between research institutions, government bodies, and international organizations exemplify the power of innovation in transforming agriculture. With an ongoing dedication to refining breeding cycles, expanding testing initiatives, and fostering collaboration, the AGG project contributes to building a sustainable and resilient agricultural future in South Asia. Early access to wheat germplasm emerges as a practical approach in this scientific endeavor, laying the foundation for a climate-resilient and food-secure region. The successes witnessed in India, Pakistan, and Nepal underscore the transformative potential of this approach, offering tangible benefits for agricultural communities in South Asia and beyond. In navigating the complexities of a changing climate and growing food demand, early access to wheat germplasm remains a pragmatic ally, propelling agricultural innovation and resilience to new heights.
CIMMYT Global Maize Program Director and CGIAR Plant Health Initiative Lead, BM Prasanna cutting a ribbon at the entrance of a new shed housing, marking the commissioning of five new seed drying machines courtesy of the of the Accelerating Genetic Gains (AGG) Project. (Photo: Susan Otieno/CIMMYT)
Kenya Agricultural and Livestock Research Organization (KALRO)’s research station at Kiboko, Kenya, where several partner institutions including the International Maize and Wheat Improvement Center (CIMMYT), conduct significant research activities on crop breeding and seed systems, is now equipped with five new seed drying machines along with a dedicated shed to house these units, a cold room for storing breeding materials, and an additional irrigation dam/reservoir. These infrastructural upgrades are worth approximately US $0.5 million.
During the commissioning of the new facilities on February 7, 2023, CIMMYT Global Maize Program Director, BM Prasanna thanked the donors, Crops to End Hunger (CtEH) Initiative and Accelerated Genetic Gains (AGG) project, that supported the upgrade of the research station, and recognized the strong partnership with KALRO.
“Today is a major milestone for CIMMYT, together with KALRO, hosting this center of excellence for crop breeding. This facility is one of the largest public sector crop breeding facilities in the world, with hundreds of hectares dedicated to crop breeding. These new facilities will enable CIMMYT and KALRO crop breeders to optimize their breeding and seed systems’ work and provide better varieties to the farming communities,” said Prasanna.
Kenya suffered one of its worst droughts ever in 2022, and the newly commissioned facilities will support expedited development of climate-resilient and nutritious crop varieties, including resistance to major diseases and pests.
Visitors at the KALRO research station in Kiboko, Kenya, looking at the newly commissioned cold room storage. (Photo: Susan Otieno/CIMMYT)
Improvements and enhancements
The efficiency of the seed driers capabilities to quickly reduce moisture content in seed from above 30% to 12% in two to three days, reducing the time taken for seed drying and allowing for more than two crop seasons per year in a crop like maize.
The additional water reservoir with a capacity of 16,500 cubic meters will eliminate irrigation emergencies and will also enhance the field research capacity at Kiboko. Reliable irrigation is essential for accelerating breeding cycles.
At the same time, the new cold room can preserve the seeds up to two years, preventing the loss of valuable genetic materials and saving costs associated with frequent regeneration of seeds.
KALRO Director General Eliud Kireger officiating the opening of the cold room storage facility at KALRO research station at Kiboko, Kenya. Looking on is CIMMYT Global Maize Program Director, BM Prasanna. (Photo: Susan Otieno/CIMMYT)
World-class research center
“The Kiboko Research Center is indeed growing into an elite research facility that can serve communities in entire sub-Saharan Africa through a pipeline of improved varieties, not only for maize but in other important crops. This will not only improve climate resilience and nutrition, but will contribute to enhanced food and income security for several million smallholder farmers,” said Prasanna.
KALRO Director General Eliud Kireger appreciated the establishment of the new facilities and thanked CIMMYT and its partners for their support.
“Today is a very important day for us because we are launching new and improved facilities for research to support breeding work and quality seed production. This research station is in Makueni County, a very dry area yet important place for research because there is adequate space, especially for breeding,” said Kireger. “We are significantly improving the infrastructure at Kiboko to produce and deliver better seed to our farmers.”
For more than three decades, CIMMYT has conducted research trials at the Kiboko Research Station, focusing on drought tolerance, nitrogen use efficiency, and resistance to pests and diseases, such as fall armyworm and stem borer. The maize Double Haploid (DH) facility established in 2013 at Kiboko, with the support of the Bill & Melinda Gates Foundation, offers DH line production service for organizations throughout Africa, and is key to increasing genetic gains in maize breeding.
Members of Umoja, Tuaminiane, Upendo and Ukombozi groundnut farming groups in Naliendele, Tanzania showing their groundnut harvests in May 2022. (Photo: Susan Otieno/CIMMYT)
The Accelerated Varietal Improvement and Seed Delivery of Legumes and Cereals in Africa (AVISA) project has developed draft national groundnut target product profiles in Malawi, Mozambique, Sudan, Tanzania, Uganda and Zambia.
Groundnut is grown in eastern and southern Africa, where it remains an important food and oil crop from small holder farmers.
The new findings from the project are a result of work from groundnut crop breeding and improvement teams from the National Agricultural Research and Extension Systems (NARES) representatives from the six largest groundnut producing countries in the eastern and southern Africa region.
Their important research was carried out with the support of representatives from the Centre for Coordination of Agricultural Research and Development for Southern Africa (CCARDESA) and CGIAR.
Developing target product profiles for groundnut
For the first time, through the International Maize and Wheat Improvement Center (CIMMYT)-led AVISA program, funded by the Bill & Melinda Gates Foundation, groundnut breeding teams discussed and documented country level priorities at a meeting in Dar es Salaam, Tanzania.
Their findings were developed using a standard target product profile template recently developed by CGIAR Excellence in Breeding (EiB) in conjunction with CGIAR’s Market Intelligence Initiative. The template serves as a tool to capture market segments and develop targeted product profiles.
The groundnut breeding teams also shared information on current groundnut production metrics and trends in the six national programs. This also helped to establish a common understanding of countries’ level research priorities.
Futhi Magagula from CCARDESA and Elailani Abdalla, Mohamed Ahmed and Abdelrazeg Badadi from ARC-Sudan deliberate on groundnut market segments for Sudan. (Photo: Biswanath Das/CIMMYT)
Agnes Gitonga, market strategist at CGIAR Genetic Innovations Action Area, who led the team in understanding and applying the template, explained that the quality of a target product profile (TPP) is dependent on how well market segments are defined. “To ensure target product profiles are an accurate reflection of customer needs, who include farmers, consumers, and processors,” she said.
“National groundnut teams nominated Country Product Design Teams that will meet nationally before the end of 2022 to review and update country TPPs. These multi-stakeholder teams will ensure that the needs of diverse groups are captured and that breeding efforts are accurately focused.”.
Harish Gandhi, Breeding Lead, Dryland Legumes and Cereals (DLC) at CIMMYT, further explained that a bottom-up approach for defining country and regional priorities was used, where each country defined market segments and target product profile based on the use of the produce and growing conditions of farmers. This strategy involved each country defining its market segments and TPP, which was based on the use of the produce and growing conditions of farmers.
Building on the draft national target product profiles that were defined at the meeting, participants went on to prioritize traits such as diseases, nutrition and stress tolerance. These factors can be critical at regional level and important in identifying potential locations for conducting phenotyping. The phenotyping locations are distributed based on capacity of stations in different countries to screen for traits, such as late leaf spot disease screening in Msekera in Zambia, which is a known hotspot for the disease.
“We had a good opportunity to consider grower needs as well as consumer needs in each country for purposes of defining the relevant groundnuts market segments. I believe this will have a positive impact on future work in groundnuts in the East and Southern Africa region,” reflected Gitonga.
The collaboration of the teams involved was a key factor for the project’s success so far and will be crucial in working towards its goals in the future.
“Involving different stakeholders in designing target product profile was an effective way of enabling transformation of individual preferences (area of interest) to collective preferences (targeted product) with consumer needs and markets in mind,” said Happy Daudi, Groundnut Breeding lead at the Tanzania Agricultural Research Institute (TARI).
Tanzania Agricultural Research Institute (TARI) Naliendele Station Groundnut Research Team ((L-R) Bakari Kidunda, Gerald Lukurugu, Anthony Bujiku and Dr. Happy Daudi) deliberate on national groundnut breeding priorities. (Photo: Biswanath Das/CIMMYT)
Strengthening groundnut breeding programs in east and southern Africa
The project’s first meeting will provide an important foundation for future research, which will use the new findings as a blueprint.
Biswanath Das, Plant Breeder, Groundnut for East and Southern Africa region and NARES Coordinator and Programming lead for EiB said, “Defining national TPPs, identifying regionally important traits and mapping a testing network are fundamental building blocks of a modern breeding program.”
At the meeting, a schedule was laid out for peer-to-peer assessments of breeding programs within the regional network to take stock of current efforts and gaps. This step helps to develop customized capacity development plans for each network partner.
“Through targeted and demand led capacity development, the East and Southern Africa groundnut crop improvement network aspires to strengthen the role of each network member in collaborative, regional breeding efforts,” Das said.
The meeting laid the ground for coordinated regional groundnut breeding and took steps towards formalizing a regional NARES-CGIAR-SME groundnut crop improvement network. By building on excellent connections that already exist among national groundnut breeding teams. Das underscored that the move will strengthen alignment of NARES, CGIAR and regional research efforts around a common vision of success.
In addition, David Okello who leads groundnut research at National Agriculture Research Organization (NARO) Uganda, noted that the meeting provided a good opportunity for consolidating the existing network. He also looked forward to welcoming more groundnut improvement programs in the region on board.
Institutionalizing Monitoring of Crop Variety Adoption using Genotyping (IMAGE) is a five-year program with the aim of establishing, institutionalizing, and scaling routine monitoring of improved variety adoption and turnover using genotyping.
It is led by country teams in Ethiopia, Nigeria and Tanzania, supported by Context Global Development and the Bill & Melinda Gates Foundation.
Reliable monitoring: IMAGE will assess the varieties that farmers are growing of four staple crops within the three target countries and marking the rate of improved variety adoption through recurring surveys and comparative analysis.
Vision for change: IMAGE supports inclusive agricultural transformation by providing insights and evidence for seed sector actors to enhance government agency capacity, improve stakeholder coordination, and lead to better resource allocation for varietal development and commercialization.
Project objectives:
Enable a national leadership mandate to monitor crop varieties and adoption
Build a network of technical experts and service providers to provide personalized advisory support
Establish best practices that enable routine monitoring and produce credible results
Form a sustainable funding mechanism based on use cases with government and stakeholder buy-in
Advocate for institutional capacity for reliable monitoring programs
IMAGE provides the opportunity to leverage past monitoring pilots and for cross-country learnings while advancing genetic reference libraries, establishing protocol adoption, and building towards institutionalization over five years. This is done through six objectives:
Comparable estimates of varietal adoption and turnover will be generated and made available to stakeholders
Standardization of best-practices and supporting technologies
Establishment of sustainable business cases
Pilot study results on varietal identity preservation in seed value chains for each country-crop combination
Institutionalized system of varietal monitoring for long-term, sustainable national partner implementation
Generated data used by seed sector stakeholders to make key decisions
Kate Dreher, Data Manager at CIMMYT, presents to scientists, technicians, data management and support teams during the training on the Enterprise Breeding System (EBS) in Nairobi, Kenya. (Photo: Susan Umazi Otieno/CIMMYT)
Scientists overseeing breeding, principal technicians and data management and support staff from the International Maize and Wheat Improvement Center (CIMMYT) learned about the Enterprise Breeding System (EBS) at a training in Nairobi, Kenya, on May 4–6, 2022. This was the first in-person training on this advanced tool held in Eastern Africa.
Kate Dreher, Data Manager at CIMMYT, was the primary trainer. Dreher sought to ensure that scientists and their teams are well equipped to confidently use the EBS for their programs, including the creation and management of trials and nurseries. During the training, participants had the opportunity to test, review and give feedback on the system.
“The EBS is an online comprehensive system that brings together different types of data, including field observations and genotypic data, to harmonize processes across all teams and enable optimized decision-making in the short term and continuous learning for the long term,” Dreher said.
She explained that the EBS is more efficient than the former approach of using the Excel-based Maize Fieldbook software, even though it managed several useful processes.
The EBS is currently available to registered breeding and support team members and data managers from CIMMYT, IITA, IRRI and AfricaRice, across all geographies where related programs are implemented. Currently, the EBS is used by programs in maize, rice and wheat crops.
A more streamlined approach
“Although teams sent germplasm and phenotypic data for centralized storage in two databases (IMIS-GMS and MaizeFinder) managed by the data management team in Mexico in the past, this required curation after the data had already been generated,” Dreher said. “The EBS will enable teams to manage their germplasm and trial nursery data directly within one system.”
The EBS stores information on germplasm and linked seed inventory items. It is also designed to house and perform analyses using phenotypic and genotypic data. Users can also capture metadata about their trials and nurseries, such as basic agronomic management information and the GPS coordinates of sites where experiments are conducted.
Yoseph Beyene, Regional Maize Breeding Coordinator for Africa and Maize Breeder for Eastern Africa at CIMMYT, observed that the training gave him firsthand information on the current capabilities and use of the live version to search germplasm and seed, and the capabilities to create nurseries and trials.
“In the AGG project, we have one primary objective which focuses on implementing improved data management, experimental designs and breeding methods to accelerate genetic gain and improved breeding efficiency. Therefore, implementing EBS is one of the top priorities for AGG project,” said Yoseph, who leads the Accelerating Genetic Gains in Maize and Wheat for Improved Livelihoods Project (AGG).
Lourine Bii, an Assistant Research Associate who recently joined CIMMYT and the only female research technician on the Global Maize program based in Kenya, also found the training useful. “The EBS is a fantastic system that enables an individual to create experiments. The system links a team, for instance a product development team, to get live updates on the various stages of creating an experiment, reducing back and forth by email.”
The system’s software development is ongoing. The development team continues to add and enhance features based on feedback from users.
Sorghum field in Kiboko, Kenya. (Photo: E Manyasa/ICRISAT)
As part of the One CGIAR reform, the Global Science Group on Genetic Innovation will implement a crop breeding and seed systems project for key crops including groundnut, sorghum and millet, across western and eastern African countries.
The International Maize and Wheat Improvement Center (CIMMYT), a leader in innovative partnerships, breeding and agronomic science for sustainable agri-food systems, will lead the project.
The Accelerated Varietal Improvement and Seed Delivery of Legumes and Cereals in Africa (AVISA) project aims to improve the health and livelihoods of millions by increasing the productivity, profitability, resilience and marketability of nutritious grain, legumes and cereal crops. The project focuses on strengthening networks to modernize crop breeding by CGIAR and national program partners, and public-private partnerships to strengthen seed systems. The project currently works in Burkina Faso, Ethiopia, Ghana, Mali, Nigeria, Uganda and Tanzania.
“Sorghum, groundnut and millets are essential staples of nutritious diets for millions of farmers and consumers and are crucial for climate-change-resilient farming systems,” explained CIMMYT Deputy Director General and Head of Genetic Resources, Kevin Pixley. “The oversight of this project by CGIAR’s Genetic Innovation Science Group will ensure continued support for the improvement of these crops in partnership with the national agricultural research and extension systems (NARES) that work with and for farmers,” he said.
“CIMMYT is delighted to lead this project on behalf of the Genetic Innovations Science Group and CGIAR,” confirms CIMMYT Director General, Bram Govaerts.
“We look forward to contributing to co-design and co-implement with partners and stakeholders the next generation of programs that leverage and build the strengths of NARES, CGIAR and others along with the research to farmers and consumers continuum to improve nutrition, livelihoods, and resilience to climate change through these crops and their cropping systems.”
Emerging in the last 120 years, science-based plant breeding begins by creating novel diversity from which useful new varieties can be identified or formed. The most common approach is making targeted crosses between parents with complementary, desirable traits. This is followed by selection among the resulting plants to obtain improved types that combine desired traits and performance. A less common approach is to expose plant tissues to chemicals or radiation that stimulate random mutations of the type that occur in nature, creating diversity and driving natural selection and evolution.
Determined by farmers and consumer markets, the target traits for plant breeding can include improved grain and fruit yield, resistance to major diseases and pests, better nutritional quality, ease of processing, and tolerance to environmental stresses such as drought, heat, acid soils, flooded fields and infertile soils. Most traits are genetically complex — that is, they are controlled by many genes and gene interactions — so breeders must intercross and select among hundreds of thousands of plants over generations to develop and choose the best.
Plant breeding over the last 100 years has fostered food and nutritional security for expanding populations, adapted crops to changing climates, and helped to alleviate poverty. Together with better farming practices, improved crop varieties can help to reduce environmental degradation and to mitigate climate change from agriculture.
Is plant breeding a modern technique?
Plant breeding began around 10,000 years ago, when humans undertook the domestication of ancestral food crop species. Over the ensuing millennia, farmers selected and re-sowed seed from the best grains, fruits or plants they harvested, genetically modifying the species for human use.
Modern, science-based plant breeding is a focused, systematic and swifter version of that process. It has been applied to all crops, among them maize, wheat, rice, potatoes, beans, cassava and horticulture crops, as well as to fruit trees, sugarcane, oil palm, cotton, farm animals and other species.
With modern breeding, specialists began collecting and preserving crop diversity, including farmer-selected heirloom varieties, improved varieties and the crops’ undomesticated relatives. Today hundreds of thousands of unique samples of diverse crop types, in the form of seeds and cuttings, are meticulously preserved as living catalogs in dozens of publicly-administered “banks.”
The International Maize and Wheat Improvement Center (CIMMYT) manages a germplasm bank containing more than 180,000 unique maize- and wheat-related seed samples, and the Svalbard Global Seed Vault on the Norwegian island of Spitsbergen preserves back-up copies of nearly a million collections from CIMMYT and other banks.
Through genetic analyses or growing seed samples, scientists comb such collections to find useful traits. Data and seed samples from publicly-funded initiatives of this type are shared among breeders and other researchers worldwide. The complete DNA sequences of several food crops, including rice, maize, and wheat, are now available and greatly assist scientists to identify novel, useful diversity.
Much crop breeding is international. From its own breeding programs, CIMMYT sends half a million seed packages each year to some 800 partners, including public research institutions and private companies in 100 countries, for breeding, genetic analyses and other research.
A field worker removes the male flower of a wheat spike, as part of controlled pollination in breeding. (Photo: Alfonso Cortés/CIMMYT)
A century of breeding innovations
Early in the 20th century, plant breeders began to apply the discoveries of Gregor Mendel, a 19th-century mathematician and biologist, regarding genetic variation and heredity. They also began to take advantage of heterosis, commonly known as hybrid vigor, whereby progeny of crosses between genetically different lines will turn out stronger or more productive than their parents.
Modern statistical methods to analyze experimental data have helped breeders to understand differences in the performance of breeding offspring; particularly, how to distinguish genetic variation, which is heritable, from environmental influences on how parental traits are expressed in successive generations of plants.
Since the 1990s, geneticists and breeders have used molecular (DNA-based) markers. These are specific regions of the plant’s genome that are linked to a gene influencing a desired trait. Markers can also be used to obtain a DNA “fingerprint” of a variety, to develop detailed genetic maps and to sequence crop plant genomes. Many applications of molecular markers are used in plant breeding to select progenies of breeding crosses featuring the greatest number of desired traits from their parents.
Plant breeders normally prefer to work with “elite” populations that have already undergone breeding and thus feature high concentrations of useful genes and fewer undesirable ones, but scientists also introduce non-elite diversity into breeding populations to boost their resilience and address threats such as new fungi or viruses that attack crops.
Transgenics are products of one genetic engineering technology, in which a gene from one species is inserted in another. A great advantage of the technology for crop breeding is that it introduces the desired gene alone, in contrast to conventional breeding crosses, where many undesired genes accompany the target gene and can reduce yield or other valuable traits. Transgenics have been used since the 1990s to implant traits such as pest resistance, herbicide tolerance, or improved nutritional value. Transgenic crop varieties are grown on more than 190 million hectares worldwide and have increased harvests, raised farmers’ income and reduced the use of pesticides. Complex regulatory requirements to manage their potential health or environmental risks, as well as consumer concerns about such risks and the fair sharing of benefits, make transgenic crop varieties difficult and expensive to deploy.
Genome editing or gene editing techniques allow precise modification of specific DNA sequences, making it possible to enhance, diminish or turn off the expression of genes and to convert them to more favorable versions. Gene editing is used primarily to produce non-transgenic plants like those that arise through natural mutations. The approach can be used to improve plant traits that are controlled by single or small numbers of genes, such as resistance to diseases and better grain quality or nutrition. Whether and how to regulate gene edited crops is still being defined in many countries.
The mobile seed shop of Victoria Seeds Company provides access to improved maize varieties for farmers in remote villages of Uganda. (Photo: Kipenz Films for CIMMYT)
Selected impacts of maize and wheat breeding
In the early 1990s, a CIMMYT methodology led to improved maize varieties that tolerate moderate drought conditions around flowering time in tropical, rainfed environments, besides featuring other valuable agronomic and resilience traits. By 2015, almost half the maize-producing area in 18 countries of sub-Saharan Africa — a region where the crop provides almost a third of human calories but where 65% of maize lands face at least occasional drought — was sown to varieties from this breeding research, in partnership with the International Institute of Tropical Agriculture (IITA). The estimated yearly benefits are as high as $1 billion.
Intensive breeding for resistance to Maize Lethal Necrosis (MLN), a viral disease that appeared in eastern Africa in 2011 and quickly spread to attack maize crops across the continent, allowed the release by 2017 of 18 MLN-resistant maize hybrids.
Improved wheat varieties developed using breeding lines from CIMMYT or the International Centre for Agricultural Research in the Dry Areas (ICARDA) cover more than 100 million hectares, nearly two-thirds of the area sown to improved wheat worldwide, with benefits in added grain that range from $2.8 to 3.8 billion each year.
Breeding for resistance to devastating crop diseases and pests has saved billions of dollars in crop losses and reduced the use of costly and potentially harmful pesticides. A 2004 study showed that investments since the early 1970s in breeding for resistance in wheat to the fungal disease leaf rust had provided benefits in added grain worth 5.36 billion 1990 US dollars. Global research to control wheat stem rust disease saves wheat farmers the equivalent of at least $1.12 billion each year.
Crosses of wheat with related crops (rye) or even wild grasses — the latter known as wide crosses — have greatly improved the hardiness and productivity of wheat. For example, an estimated one-fifth of the elite wheat breeding lines in CIMMYT international yield trials features genes from Aegilops tauschii, commonly known as “goat grass,” that boost their resilience and provide other valuable traits to protect yield.
Biofortification — breeding to develop nutritionally enriched crops — has resulted in more than 60 maize and wheat varieties whose grain offers improved protein quality or enhanced levels of micro-nutrients such as zinc and provitamin A. Biofortified maize and wheat varieties have benefited smallholder farm families and consumers in more than 20 countries across sub-Saharan Africa, Asia, and Latin America. Consumption of provitamin-A-enhanced maize or sweet potato has been shown to reduce chronic vitamin A deficiencies in children in eastern and southern Africa. In India, farmers have grown a high-yielding sorghum variety with enhanced grain levels of iron and zinc since 2018 and use of iron-biofortified pearl millet has improved nutrition among vulnerable communities.
Innovations in measuring plant responses include remote sensing systems, such as multispectral and thermal cameras flown over breeding fields. In this image of the CIMMYT experimental station in Obregón, Mexico, water-stressed plots are shown in green and red. (Photo: CIMMYT and the Instituto de Agricultura Sostenible)
Thefuture
Crop breeders have been laying the groundwork to pursue genomic selection. This approach takes advantage of low-cost, genome-wide molecular markers to analyze large populations and allow scientists to predict the value of particular breeding lines and crosses to speed gains, especially for improving genetically complex traits.
Speed breeding uses artificially-extended daylength, controlled temperatures, genomic selection, data science, artificial intelligence tools and advanced technology for recording plant information — also called phenotyping — to make breeding faster and more efficient. A CIMMYT speed breeding facility for wheat features a screenhouse with specialized lighting, controlled temperatures and other special fixings that will allow four crop cycles — or generations — to be grown per year, in place of only two cycles with normal field trials. Speed breeding facilities will accelerate the development of productive and robust varieties by crop research programs worldwide.
Data analysis and management. Growing and evaluating hundreds of thousands of plants in diverse trials across multiple sites each season generates enormous volumes of data that breeders must examine, integrate, and co-analyze to inform decisions, especially about which lines to cross and which populations to discard or move forward. New informatics tools such as the Enterprise Breeding System will help scientists to manage, analyze and apply big data from genomics, field and lab studies.
Following the leaders. Driven by competition and the quest for profits, private companies that market seed and other farm products are generally on the cutting edge of breeding innovations. The CGIAR’s Excellence in Breeding (EiB) initiative is helping crop breeding programs that serve farmers in low- and middle-income countries to adopt appropriate best practices from private companies, including molecular marker-based approaches, strategic mechanization, digitization and use of big data to drive decision making. Modern plant breeding begins by ensuring that the new varieties produced are in line with what farmers and consumers want and need.
Cover photo: CIMMYT experimental station in Toluca, Mexico. Located in a valley at 2,630 meters above sea level with a cool and humid climate, it is the ideal location for selecting wheat materials resistant to foliar diseases, such as wheat rust. Conventional plant breeding involves selection among hundreds of thousands of plants from crosses over many generations, and requires extensive and costly field, screenhouse and lab facilities. (Photo: Alfonso Cortés/CIMMYT)
Dryland Crops, formerly known as the Accelerated Varietal Improvement and Seed Systems in Africa (AVISA) project, aims to improve the livelihoods of small-scale producers and consumers of sorghum, millet, groundnut, cowpea and bean. Project partners focus on improving the breeding and seed systems of these crops in their key geographies in Burkina Faso, Ethiopia, Ghana, Mali, Nigeria, Tanzania and Uganda. Other crops receiving growing attention in the project include finger millet, pigeon pea and chickpea.
Although significant adoption of improved seed of dryland cereals and legume crops in Africa has been reported, its overall use remains low. There is a growing interest in these crops, particularly because of their resilience to climate-change; however, the seed sector is constrained by lack of product information, dearth of knowledge of the size and scale of the business opportunity, and inadequate access to early generation seed.
Dryland Crops will address these constraints by contributing to the establishment of robust systems that:
Enable networks to work synergistically across countries with common challenges and opportunities.
Support national agricultural research systems to access research, professional development and infrastructure-building opportunities.
Increase the quantity and quality of data substantiating varietal superiority and the demand for seed and grain of improved varieties.
Boost the availability of early generation seed and strengthen links between the research system and private- and public-sector actors.
The aspiration is to codevelop, validate by co-implementation, and continuously improve with partners research-to-farm-to-consumer models that achieve positive impacts on farmers’ livelihoods and consumers’ wellbeing.
The Alliance of Bioversity and CIAT and IITA will lead initiatives for common bean and cowpea, respectively. For sorghum, pearl millet and groundnut breeding, CIMMYT will design programs that support crop improvement networks, including CGIAR and national agricultural research systems, and incorporate best approaches, principles, and tools, particularly those availed through the Excellence in Breeding (EiB) platform.
The project is committed to gender equity as a guiding principle, considering the critical role women play in choosing legume and cereal varieties and seed sources. Women seed entrepreneurs and women-led seed companies will garner special attention for capacity development. Partnerships with actors through the value chain, platforms and demonstrations will ensure women have equal access to improved technologies.
The previous phase of the AVISA project was led by the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT).
Last month, the CGIAR Excellence in Breeding (EiB) platform handed over digitization equipment to the Kenya Agricultural and Livestock Research Organization (KALRO) as part of ongoing efforts to modernize the public agency’s crop breeding programs. The handover of the equipment, valued at roughly $85,000, took place at KALRO headquarters in Nairobi on March 8, 2021, with representatives from the International Maize and Wheat Improvement Center (CIMMYT), EiB and KALRO in attendance.
KALRO received 23 units of equipment including seed counters, label printers, handheld data collectors, tablets and package printers. These will help the organization speed up and enhance the accuracy of various breeding processes, including seed preparation, data collection and data analysis. They will also support inventory management within KALRO’s maize, wheat, rice, sorghum, bean, soybean and potato breeding programs at six of its research centers in Kenya.
(L-R) CIMMYT Regional Representative for Africa and Kenya Country Representatives Moses Siambi, CGIAR EiB NARS Coordinator Biswanath Das, KALRO Director General Eliud Kireger and KALRO Deputy Director General for Crops Felister Makini at the digitization equipment handover event in Nairobi, Kenya. (Photo: Joshua Masinde/CIMMYT)
Dispensing with laborious systems
A lack of digitization equipment hampers the research efforts of many national agricultural research systems (NARS) across Africa. This adverse situation is compounded by unreliable institutional memory, which constrains NARS efforts to breed an assortment of crop varieties efficiently.
“Currently, KALRO uses very laborious systems including manual layouts and collection, followed by manual data entry into computers. This old age process is prone to data entry errors and delays in analysis, publication and reporting,” says KALRO Director General Eliud Kireger.
“With the equipment we are receiving, information and data can be recalled by a click of a button. The equipment will also significantly reduce research costs related to labor, thus freeing our scientists to focus on core research activities.”
The equipment will also support KALRO’s ongoing efforts to digitize its historical data, especially for the maize and wheat programs using the Breeding Management System (BMS). So far, 20 years of maize historical data has been uploaded onto the BMS platform for ease of access.
Prepped for emerging challenges
The CGIAR EiB platform was established in 2017 to help modernize public breeding programs in the CGIAR and NARS to increase their rates of genetic gain. In recent years, there has been an upsurge in challenges including climate change, population growth, rapid urbanization, changing dietary inclinations, transboundary movement of pests and diseases. These have exerted an enormous strain on food production systems and elicited the urgency to prioritize the adoption of new plant breeding techniques and technologies to address current and emerging threats. This calls for a holistic approach to tackle the issues including better agronomy and policy, according to EiB NARS Coordinator Biswanath Das.
“Modernizing our plant breeding programs to develop new, climate smart, market driven varieties will be at the heart of the solution,” says Das. “We must ensure that public plant breeding programs are not left behind because for many crops in Africa, there is limited private sector interest. Public breeding programs must shoulder the responsibility for ensuring the development and adoption of the next generation of crop varieties.”
CGIAR EiB NARS Coordinator Biswanath Das shares remarks at the digitization handover event in Nairobi, Kenya. (Photo: Joshua Masinde/CIMMYT)
Already, KALRO breeding programs, in collaboration with international CGIAR centers, have played a leading role in supporting farmers in sub–Saharan Africa to address many emerging plant threats such as wheat rust (UG99), maize lethal necrosis (MLN) and fall armyworm.
As part of its commitment to supporting NARS partners, EiB provided over 10 million Kenyan shillings ($92,000) worth of material and in-kind support to various KALRO breeding operations in 2020. This included genotyping support for maize and wheat, support to adopt the BMS digital data management system, technical support and training of KALRO breeders. Much of the digitization work is driven by EiB’s Operations and Phenoytyping module, led by Gustavo Teixeira. “We’ll continue to consider a whole range of devices and solutions,” says Teixeira. “It’s a part of our culture of continuous improvement, so breeding programs can focus on what really adds value to their clients.”
EiB will continue to support NARS across Africa and beyond to digitize their operations, and is working with partners to secure more equipment, training and resources. With this digitization project, EiB has targeted 24 breeding programs in 14 African countries. These include programs run by AfricaRice, CIMMYT, the International Institute of Tropical Agriculture (IITA) and the International Rice Research Institute (IRRI).
“We want to do more to support centers to improve their operations so they can achieve the most effective and cost efficient phenotypic processes — agronomic practices, seed processing and other areas,” explains Teixeira. “We aim to expand to more programs and partners.”
Matthew Reynolds, Distinguished Scientist and Head of Wheat Physiology at CIMMYT, talked to The Guardian producer Patrick Greenfield about the process to create climate- and heat-resistant crops.
East African Seed Company has a rich history of nearly 50 years, serving farmers with improved climate-resilient seed varieties. Established in 1972, the company produces and sells improved seed, through a wide distribution network in at least 15 countries in sub-Saharan Africa. It also markets agrochemicals and other farm inputs, and has ambitions of expanding to the rest of Africa, trading as Agriscope Africa Limited.
Smallholder farmers in sub-Saharan Africa continue to face multiple biotic and abiotic stresses as they try to improve their farms’ productivity and their livelihoods. Maize seed that guarantees high yield is a key trait, coupled with other key attributes such as drought tolerance, disease and pest resistance, early seedling vigor as well as suitability for food and animal feed.
With the varieties serving both small- and large-scale commercial farmers, challenges such as the fall armyworm, diminishing soil fertility and erratic rains have persisted in recent years and remain as key farming obstacles. “Such challenges diminish crop production and the grain quality thereby, lessening farmers’ profitability,” says Rogers Mugambi, Chief Operating Officer of East African Seed Company.
Scientists at the International Maize and Wheat Improvement Center (CIMMYT), in collaboration with partners in the national agriculture research systems and the commercial seed sector, continue to develop seed varieties that can guarantee decent yield even in times of climatic, disease and pest stress.
General view of the East African Seed warehouse. (Photo: Jerome Bossuet/CIMMYT)
Top-notch research trickles down to farmers
Over the years, East African Seed has inked partnerships with CIMMYT, national research institutes and other agencies in the countries where it operates. Such partnerships have been the driving force to its success and the impacts within the farming communities in sub-Saharan Africa.
“Our collaboration with CIMMYT began in 2008 with germplasm acquisition. The cooperation has expanded to include testing networks for new hybrids, early-generation seed production and marketing. The overall beneficiary is the smallholder farmer who can access quality seeds and produce more with climate-smart products,” Mugambi says.
Apart from the multi-stress-tolerant varieties developed and released over time by the national agricultural research programs, CIMMYT recently announced a breakthrough: fall armyworm-tolerant elite maize hybrids for eastern and southern Africa. This success followed three years of rigorous research and experiments conducted in Kenya and signified a key milestone in the fight against fall armyworm.
As part of the partnership in the Drought Tolerant Maize for Africa (DTMA) and Stress Tolerant Maize for Africa (STMA) projects, East African Seed Company (Agriscope Africa Limited) established demonstration farms and conducted field days in Kenya, reaching thousands of farmers as a result. It was also able to produce early generation seed, which supported production of 2,000 metric tons of certified seed. This partnership now continues in the Accelerating Genetic Gains in Maize and Wheat (AGG) project.
The company has contracted large- and small-scale growers across the country to meet its seed production targets.
“Most of our small-scale growers are clustered in groups of up to 30 farmers with less than five acres of farmland. The large growers have advanced irrigation facilities such as the pivot system and seed processing plants. The seed from the fields is pre-cleaned and dried in the out-grower facilities before delivery to our factory for further cleaning and processing,” Mugambi explains.
A handful of improved maize seed from the drought-tolerant variety TAN 250, developed and registered for sale in Tanzania through CIMMYT’s Drought Tolerant Maize for Africa (DTMA) project. (Photo: Anne Wangalachi/CIMMYT)
Out with the drought
Currently, of the 1,300 metric tons of drought-tolerant hybrid seeds it produces yearly, 500 metric tons constitute those derived from the partnership in the STMA project. Two notable hybrids, HODARI (MH501) and TOSHEKA (MH401), were derived during the DTMA and STMA projects. Released in 2014 and accepted for regional certification through the Common Market for Eastern and Southern Africa (COMESA)’s regional catalogue, the MH501 is a mid-altitude adapted and medium maturing three-way cross hybrid. The yield advantage of 15% over the local commercial checks triggered widespread adoption by the farmers, according to Mugambi. In Kenya, it was used as a commercial check during national performance trials, from 2017 to 2019.
The MH401, an early maturing hybrid with moderate drought tolerance, has been adopted in lowland and mid-altitude dry ecologies of Kenya and Tanzania. It has a 20% yield advantage over the local commercial checks.
As part of its varietal replacement, East African Seed Company looks to steadily retire older varieties such as KH600-15A and WE1101 and promote new ones including TAJIRI (EASH1220), TAJI (MH502) and FARAJA (MH503).
To promote new varieties and successfully reach smallholders, the company conducts field days, farm-level varietal demonstrations, road shows and radio programs. It also disseminates information on the benefits of new varieties while also dispensing promotional materials such as branded t-shirts and caps.
“Additionally, we organize annual field days at our research farm in Thika, where key and influential farmers and other stakeholders are invited from across Kenya and neighboring countries to learn about our new agricultural technologies,” Mugambi says.
The food security and livelihoods of smallholder farming families in sub-Saharan Africa depend on maize production. The region accounts for up to two-thirds of global maize production, but is facing challenges related to extreme weather events, climate-induced stresses, pests and diseases, and deteriorating soil quality. These require swift interventions and innovations to safeguard maize yields and quality.
In this Q&A, we reflect on the results and impact of the long-term collaborative work on drought-tolerant maize innovations spearheaded by two CGIAR Research Centers: the International Maize and Wheat Improvement Center (CIMMYT) and International Institute of Tropical Agriculture (IITA). This innovative work has changed guises over the years, from the early work of the Drought Tolerant Maize for Africa (DTMA) and Drought Tolerant Maize for Africa Seed Scaling (DTMASS) projects through later iterations such as Stress Tolerant Maize for Africa (STMA) and the newest project, Accelerating Genetic Gains in Maize and Wheat (AGG).
In this Q&A, three leaders of this collaborative research reflect on the challenges their work has faced, the innovations and impact it has generated for smallholder farmers, and possible directions for future research. They are: B.M Prasanna, director of CIMMYT’s Global Maize Program and of the CGIAR Research Program on Maize (MAIZE); Abebe Menkir, a maize breeder and maize improvement lead at IITA; and Cosmos Magorokosho, project lead for AGG-Maize at CIMMYT.
Briefly describe the challenges confronting small-scale farmers prior to the introduction of drought-tolerant maize and how CIMMYT and IITA responded to these challenges?
B.M.P.: Maize is grown on over 38 million hectares in sub-Saharan Africa, accounting for 40% of cereal production in the region and providing at least 30% of the population’s total calorie intake. The crop is predominantly grown under rainfed conditions by resource-constrained smallholder farmers who often face erratic rainfall, poor soil fertility, increasing incidence of climatic extremes — especially drought and heat — and the threat of devastating diseases and insect pests.
Around 40% of maize-growing areas in sub-Saharan Africa face occasional drought stress with a yield loss of 10–25%. An additional 25% of the maize crop suffers frequent drought, with yield losses of up to 50%. Climate change is further exacerbating the situation, with devastating effects on the food security and livelihoods of the millions of smallholder farmers and their families who depend on maize in sub-Saharan Africa. Therefore, the improved maize varieties with drought tolerance, disease resistance and other farmer-preferred traits developed and deployed by CIMMYT and IITA over the last ten years in partnership with an array of national partners and seed companies across sub-Saharan Africa are critical in effectively tackling this major challenge.
A.M.: Consumption of maize as food varies considerably across sub-Saharan Africa, exceeding 100 kg per capita per year in many countries in southern Africa. In years when rainfall is adequate, virtually all maize consumed for food is grown in sub-Saharan Africa, with a minimal dependence on imported grain. Maize production, however, is highly variable from year to year due to the occurrence of drought and the dependence of national maize yields on seasonal rainfall. One consequence has been widespread famine occurring every five to ten years in sub-Saharan Africa, accompanied by large volumes of imported maize grain as food aid or direct imports.
This places a significant strain on resources of the World Food Programme and on national foreign exchange. It also disincentivizes local food production and may not prevent or address cyclical famine. It also leaves countries ill-equipped to address famine conditions in the period between the onset of the crisis and the arrival of food aid. Investment in local production, which would strengthen the resilience and self-sufficiency in food production of smallholder farming families, is a far better option to mitigate food shortages than relying on food aid and grain imports.
C.M.: Smallholder farmers in sub-Saharan Africa face innumerable natural and socioeconomic constraints. CIMMYT, in partnership with IITA and national agricultural research system partners, responded by developing and catalyzing the commercialization of new maize varieties that produce reasonable maize yields under unpredictable rainfall-dependent growing season.
Over the life of the partnership, more than 300 new climate-adaptive maize varieties were developed and released in more than 20 countries across sub-Saharan Africa where maize is a major staple food crop. Certified seed of over 100 stress-tolerant improved maize varieties have been produced by seed company partners, reaching more than 110,000 tons in 2019. The seeds of these drought-tolerant maize varieties have benefited more than 8 million households and were estimated to be grown on more than 5 million hectares in eastern, southern and west Africa in 2020.
A farmer in Mozambique stands for a photograph next to her drought-tolerant maize harvest. (Photo: CIMMYT)
In what ways did the drought-tolerant maize innovation transform small-scale farmers’ ability to respond to climate-induced risks? Are there any additional impacts on small scale farmers in addition to climate adaptation?
B.M.P.: The elite drought-tolerant maize varieties can not only provide increased yield in drought-stressed crop seasons, they also offer much needed yield stability. This means better performance than non-drought-tolerant varieties in both good years and bad years to a smallholder farmer.
Drought-tolerant maize varieties developed by CIMMYT and IITA demonstrate at least 25-30% grain yield advantage over non-drought-tolerant maize varieties in sub-Saharan Africa under drought stress at flowering. This translates into at least a 1 ton per hectare enhanced grain yield on average, as well as reduced downside risk in terms of lost income, food insecurity and other risks associated with crop yield variability. In addition to climate adaptation, smallholder farmers benefit from these varieties due to improved resistance to major diseases like maize lethal necrosis and parasitic weeds like Striga. We have also developed drought-tolerant maize varieties with enhanced protein quality — such as Quality Protein Maize or QPM — and provitamin A, which improve nutritional outcomes.
We must also note that drought risk in sub-Saharan Africa has multiple and far-reaching consequences. It reduces incentives for smallholder farmers to intensify maize-based systems and for commercial seed companies to invest and evolve due to a limited seed market.
Drought-tolerant maize is, therefore, a game changer as it reduces the downside risk for both farmers and seed companies and increases demand for improved maize seed, thus strengthening the commercial seed market in sub-Saharan Africa. Extensive public-private partnerships around drought-tolerant maize varieties supported the nascent seed sector in sub-Saharan Africa and has enabled maize-based seed companies to significantly grow over the last decade. Seed companies in turn are investing in marketing drought-tolerant maize varieties and taking the products to scale.
A.M.: The DTMA and STMA projects were jointly implemented by CIMMYT and IITA in partnership with diverse national and private sector partners in major maize producing countries in eastern, southern and western Africa to develop and deploy multiple stress-tolerant and productive maize varieties to help farmers adapt to recurrent droughts and other stresses including climate change.
These projects catalyzed the release and commercialization of numerous stress-resilient new maize varieties in target countries across Africa. Increasing the resilience of farming systems means that smallholder farmers need guaranteed access to good quality stress resilient maize seeds. To this end, the two projects worked with public and private sector partners to produce large quantities of certified seeds with a continual supply of breeder seeds from CIMMYT and IITA. The availability of considerable amount of certified seeds of resilient maize varieties has enabled partners to reach farmers producing maize under stressful conditions, thus contributing to the mitigation of food shortages that affect poor people the most in both rural and urban areas.
C.M.: The drought-tolerant maize innovation stabilized maize production under drought stress conditions in sub-Saharan Africa countries. Recent study results showed that households that grew drought-tolerant maize varieties had at least half a ton more maize harvest than the households that did not grow the drought-tolerant maize varieties, thus curbing food insecurity while simultaneously increasing farmers’ economic benefits. Besides the benefit from drought-tolerant innovation, the new maize varieties developed through the partnership also stabilized farmers’ yields under major diseases, Striga infestation, and poor soil fertility prevalent in sub-Saharan Africa.
How is the project addressing emerging challenges in breeding for drought-tolerant maize and what opportunities are available to address these challenges in the future?
Margaret holds an improved ear of drought-tolerant maize. Margaret’s grandmother participated in an on-farm trial in Murewa district, 75 kilometers northeast of Zimbabwe’s capital Harare. (Photo: Jill Cairns/CIMMYT)
B.M.P.: A strong pipeline of elite, multiple-stress-tolerant maize varieties — combining other relevant adaptive and farmer-preferred traits — has been built in sub-Saharan Africa through a strong germplasm base, partnerships with national research partners and small- and medium-sized seed companies, an extensive phenotyping and multi-location testing network, and engagement with farming communities through regional on-farm trials for the identification of relevant farmer-preferred products.
CGIAR maize breeding in sub-Saharan Africa continues to evolve in order to more effectively and efficiently create value for the farmers we serve. We are now intensively working on several areas: (a) increasing genetic gains (both on-station and on-farm) through maize breeding in the stress-prone environments of sub-Saharan Africa by optimizing our breeding pipelines and effectively integrating novel tools, technologies and strategies (e.g., doubled haploids, genomics-assisted breeding, high-throughput and precise phenotyping, improved breeding data management system, etc.); (b) targeted replacement of old or obsolete maize varieties in sub-Saharan Africa with climate-adaptive and new varieties; (c) developing next-generation climate-adaptive maize varieties with traits such as native genetic resistance to fall armyworm, and introgressed nutritional quality traits (e.g., provitamin A, high Zinc) to make a positive impact on the nutritional well-being of consumers; and (d) further strengthening the breeding capacity of national partners and small and medium-sized seed companies in sub-Saharan Africa for a sustainable way forward.
A.M.: The DTMA and STMA projects established effective product pipelines integrating cutting-edge phenotyping and molecular tools to develop stress-resilient maize varieties that are also resistant or tolerant to MLN disease and fall armyworm. These new varieties are awaiting release and commercialization. Increased investment in strengthening public and private sector partnerships is needed to speed up the uptake and commercialization of new multiple stress-resilient maize varieties that can replace the old ones in farmers’ fields and help achieve higher yield gains.
Farmers’ access to new multiple-stress-tolerant maize varieties will have a significant impact on productivity at the farm level. This will largely be due to new varieties’ improved response to fertilizer and favorable growing environments as well as their resilience to stressful production conditions. Studies show that the adoption of drought-tolerant maize varieties increased maize productivity, reduced exposure to farming risk among adopters and led to a decline in poverty among adopters. The availability of enough grain from highly productive and stress-resilient maize varieties can be the cheapest source of food and release land to expand the cultivation of other crops to facilitate increased access to diversified and healthy diets.
C.M.: The project is tackling emerging challenges posed by new diseases and pests by building upon the successful genetic base of drought-tolerant maize. This is being done by breeding new varieties that add tolerance to the emerging disease and pest challenges onto the existing drought-tolerant maize backgrounds. Successes have already been registered in breeding new varieties that have high levels of resistance to MLN disease and the fall armyworm pest.
Opportunities are also available to address new challenges including: pre-emptively breeding for threats to maize production challenges that exist in other regions of the world before these threats reach sub-Saharan Africa; enhancing the capacity of national partners to build strong breeding programs that can address new threats once they emerge in sub-Saharan Africa; and sharing knowledge and novel high-value breeding materials across different geographies to immediately address new threats once they emerge.
Cover photo: Alice Nasiyimu stands in front of a drought-tolerant maize plot at her family farm in Bungoma County, in western Kenya. (Photo: Joshua Masinde/CIMMYT)
A CIMMYT technician cuts a leaf sample for DNA extraction. (Photo: CIMMYT)
Wheat breeders from across the globe took a big step towards modernizing their molecular breeding skills at a recent workshop sponsored by the Wheat Initiative, with the CGIAR Excellence in Breeding Platform (EiB) and the International Maize and Wheat Improvement Center (CIMMYT).
The workshop focused on three open-source tools used in molecular breeding: GOBii-GDM for genomic data management, Flapjack for data visualization and breeding analysis, and Galaxy for Genomic Selection. These tools help breeders make selections more quickly and precisely, and ultimately lead to more cost effective and efficient improvement of varieties.
The Wheat Initiative — a global scientific collaboration whose goals are to create improved wheat varieties and disseminate better agronomic practices worldwide — and its Breeding Methods and Strategies expert working group had planned to host these trainings during the 2020 Borlaug Global Rust Initiative Technical Workshop in the United Kingdom. After it became obvious that in-person trainings were not possible, the course organizers — including CIMMYT molecular wheat breeder Susanne Dreisigacker and EiB Adoption Lead and former GOBii project director Elizabeth Jones — decided to come together to host online workshops.
Many of the tools will be incorporated into EiB’s Enterprise Breeding System (EBS), a new integrated data management system being developed for CGIAR breeders. Jones plans to also design training modules for these molecular breeding tools that will be accessible to anyone through the EiB Toolbox.
The first session of the workshop “Transforming Wheat Breeding Through Integrated Data Management with GOBii and Analysis in Flapjack” benefited breeders from Australia, Canada, Ethiopia, France, India, Ireland, Italy, Morocco, Pakistan, Switzerland, Tunisia, the United Kingdom and the United States.
Susanne Dreisigacker presents during one of the sessions of the workshop.
Powering data analysis around the world
The workshop series, “Transforming Wheat Breeding Through Integrated Data Management with GOBii and Analysis in Flapjack,” aimed to benefit breeders from wheat producing countries all over the world, with sessions over two different time zones spread out over three days to reduce “Zoom fatigue.” Participants joined the first session from Australia, Canada, Ethiopia, France, India, Ireland, Italy, Morocco, Pakistan, Switzerland, Tunisia, the United Kingdom and the United States.
“It was wonderful to see the diversity of participants that we were able to train through an online workshop, many of whom otherwise might not have been able to travel to the UK for the original meeting,” said Jones. “Participants were very engaged, making the workshop so rewarding.”
The workshop was guided by Teresa Saavedra, Wheat Initiative coordinator. Apart from Dreisigacker and Jones, other trainers explained specific tools and approaches. Iain Milne from the James Hutton Institute in Scotland gave more details about the Flapjack genotyping visualization tool, which includes analysis for pedigree verification, marker assisted backcrossing and forward breeding. Andrew Kowalczyk, developer at Diversity Arrays Technology, spoke about the genotyping data QC tool DArTView.
A CIMMYT technician performs one of the steps to extract DNA samples from plants. (Photo: CIMMYT)
Clay Sneller, wheat breeder at Ohio State University, contributed training materials for important molecular breeding tools. Carlos Ignacio, previously based at the International Rice Research Center (IRRI) and now working on a PhD in Genomic Selection at Ohio State University, contributed his experience as a GOBii team member and a major contributor towards the design of Flapjack tools. Star Gao, application specialist with GOBii and now a requirements analyst for the Enterprise Breeding System, also facilitated the sessions.
Gilles Charmet, research director at the France’s National Research Institute for Agriculture, Food and Environment (INRAE), introduced the sessions in the Americas/Europe time zone with welcome remarks and overview of the goals of the Wheat Initiative. Alison Bentley, director of the CIMMYT Global Wheat Program, briefed on the achievements and goals of the CIMMYT Wheat program and the Accelerating Genetic Gains in Maize and Wheat for Improved Livelihoods (AGG) project.
“This training will contribute towards us reaching our AGG goals of accelerating gains in wheat, by sharing technical knowledge, and allowing our beneficiary partners to have state-of-the-art know-how in the use of genetic and genomic data,” Bentley said.
Participant Stéphane Boury from Caussade Semences, France commented, “This was a very effective way to learn about new tools in wheat breeding.”
The sessions continue in Australasia next week, and will be introduced by Peter Langridge, chair of the Scientific Board for the Wheat Initiative, and EiB director Michael Quinn. Sanjay Kumar Singh, incoming chair of the Breeding expert working group for the Wheat Initiative, will close the event.
The challenges facing our food system are growing, both in size and in complexity. In order to tackle these issues and meet the needs of our changing world, the International Maize and Wheat Improvement Center (CIMMYT) understands the importance of assembling a workforce that is diverse, creative and representative. In addition to encouraging STEM careers and hiring more women in scientific positions, we must also foster a more encouraging scientific community for women whose careers are just sparking.
Whether it is through a school field trip, a first internship or a PhD thesis project, CIMMYT is committed to encouraging young women to step into the lab and the fields, and up to the challenge, as we strive to create a more equitable community. On the International Day of Women and Girls in Science, we are inspired by the words of some of the many brilliant women whose scientific careers are just beginning, lighting the pathway to a more equitable future.
The International Day of Women and Girls in Science is particularly meaningful to CIMMYT’s new Global Wheat Program (GWP) Director, Alison Bentley. Listen and watch as she tells her story, from her first lightbulb moment on a high school field trip, to a leadership position in the wheat research world.
In celebration of the International Day of Women and Girls in Science, CIMMYT is participating in a unique marathon event, carrying a global conversation with CGIAR women scientists that are leading change and creating solutions to some of the world’s biggest challenges.
Powered by Women in Research and Science (WIRES), a new employee-led resource group at CGIAR, the event will showcase the many ways women scientists are transforming the way we look at our food, land and water systems around the world. In addition to learning about cutting-edge science, you’ll be able to engage with inspiring speakers in 13 different countries.
Join CIMMYT’s discussion on February 11, 2021, at 1:00 p.m. CST, and learn about the journeys of the 2020 Bänziger Award recipients, an engaging Q&A with four CIMMYT scientists, and our vision for a more equitable workforce. Register for the event.
By adopting best practices and established modern tools, national agricultural research systems (NARS) are making data-driven decisions to boost genetic improvement. And they are measuring this progress through tracking and setting goals around “genetic gain.”
Genetic gain means improving seed varieties so that they have a better combination of genes that contribute to desired traits such as higher yields, drought resistance or improved nutrition. Or, more technically, genetic gain measures, “the expected or realized change in average breeding value of a population over at least one cycle of selection for a particular trait of index of traits,” according to the CGIAR Excellence in Breeding (EiB)’s breeding process assessment manual.
CGIAR breeders and their national partners are committed to increasing this rate of improvement to at least 1.5% per year. So, it has become a vital and universal high-level key performance indicator (KPI) for breeding programs.
“We are moving towards a more data-driven culture where decisions are not taken any more based on gut feeling,” EiB’s Eduardo Covarrubias told nearly 200 NARS breeders in a recent webinar on Enhancing and Measuring Genetic Gain. “Decisions that can affect the sustainability and the development of organization need to be based on facts and data.”
Improved metrics. Better decisions. More and better food. But how are NARS positioned to better measure and boost the metric?
EiB researchers have been working with both CGIAR breeding programs and NARS to broaden the understanding of genetic gain and to supply partners with methods and tools to measure it.
The recent webinar, co-sponsored by EiB and the CIMMYT-led Accelerating Genetic Gains in Maize and Wheat (AGG) project, highlighted tools and services that NARS are accessing, such as genotyping, data analysis and mechanization.
Through program assessments, customized expert advice, training and provision of services and resources, EiB researchers are helping national partners arrive at the best processes for driving and measuring genetic gains in their programs.
For example, the EiB team, through Crops to End Hunger (CtEH), is providing guidelines to breeders to help them maximize the accuracy and precision, while reducing the cost of calculating genetic gains. The guidelines make recommendations such as better design of trials and implementing an appropriate check strategy that permits regular and accurate calculation of genetic gain.
A comprehensive example at the project level is EiB’s High-Impact Rice Breeding in East and West Africa (Hi-Rice), which is supporting the modernization of national rice programs in eight key rice-producing countries in Africa. Hi-Rice delivers training and support to modernize programs through tools such as the use of formalized, validated product profiles to better define market needs, genotyping tools for quality control, and digitizing experiment data to better track and improve breeding results. This is helping partners replace old varieties of rice with new ones that have higher yields and protect against elements that attack rice production, such as drought and disease. Over the coming years, EiB researchers expect to see significant improvements in genetic gain from the eight NARS program partners.
And in the domain of wheat and maize, AGG is working in 13 target countries to help breeders adopt best practices and technologies to boost genetic gain. Here, the EiB team is contributing its expertise in helping programs develop their improvement plans — to map out where, when and how programs will invest in making changes.
NARS and CGIAR breeding programs also have access to tools and expertise on adopting a continuous improvement process — one that leads to cultural change and buy-in from leadership so that programs can identify problems and solve them as they come up. Nearly 150 national breeding partners attended another EiB/AGG webinar highlighting continuous improvement key concepts and case studies.
National programs are starting to see the results of these partnerships. The Kenya Agricultural & Livestock Research Organization (KALRO)’s highland maize breeding program has undertaken significant changes to its pipelines. KALRO carried out its first-ever full program costing, and based on this are modifying their pipeline to expand early stage testing. They are also switching to a double haploid breeding scheme with support from the CGIAR Research Program on Maize (MAIZE), in addition to ring fencing their elite germplasm for future crosses.
KALRO has also adopted EiB-supported data management tools, and are working with the team to calculate past rates of genetic gains for their previous 20 years of breeding. These actions — and the resulting data — will help them decide on which tools and methods to adopt in order to improve the rate of genetic gain for highland maize.
“By analyzing historical genetic gain over the last 20 years, it would be interesting to determine if we are still making gains or have reached a plateau,” said KALRO’s Dickson LIgeyo, who presented a Story of Excellence at EiB’s Virtual Meeting 2020. “The assessment will help us select the right breeding methods and tools to improve the program.”
Other NARS programs are on a similar path to effectively measure and increase genetic gain. In Ghana, the rice breeding program at Council for Scientific and Industrial Research (CSIR) have developed product profiles, identified their target market segments, costed out their program, digitized their operations, and have even deployed molecular markers for selection.
With this increased expertise and access to tools and services, national breeding programs are set to make great strides on achieving genetic gain goals.
“NARS in Africa and beyond have been aggressively adopting new ideas and tools,” says EiB’s NARS engagement lead Bish Das. “It will pay a lot of dividends, first through the development of state-of-the-art, and ultimately through improving genetic gains in farmers’ fields. And that’s what it’s all about.”
